Evaluation of Clean Fractionation Pretreatment for the Production of Renewable Fuels and Chemicals from Corn Stover

2014 ◽  
Vol 2 (6) ◽  
pp. 1364-1376 ◽  
Author(s):  
Rui Katahira ◽  
Ashutosh Mittal ◽  
Kellene McKinney ◽  
Peter N. Ciesielski ◽  
Bryon S. Donohoe ◽  
...  
Keyword(s):  
Corn Stover ◽  
Renewable Fuels ◽  
Fuels And Chemicals

Evaluation of microbial triglyceride oil purification requirements for the CelTherm process: an efficient biochemical pathway to renewable fuels and chemicals

2014 ◽  
Vol 37 (10) ◽  
pp. 2121-2129 ◽  
Author(s):  
Michael Linnen ◽  
Wayne Seames ◽  
Alena Kubatova ◽  
Suresh Menon ◽  
Kashinatham Alisala ◽  
...  
Keyword(s):  
Renewable Fuels ◽  
Biochemical Pathway ◽  
Fuels And Chemicals ◽  
Oil Purification

2-Keto acids based biosynthesis pathways for renewable fuels and chemicals

2014 ◽  
Vol 42 (3) ◽  
pp. 361-373 ◽  
Author(s):  
Yohei Tashiro ◽  
Gabriel M. Rodriguez ◽  
Shota Atsumi
Keyword(s):  
Renewable Fuels ◽  
Keto Acids ◽  
Fuels And Chemicals

scholarly journals Hydrodynamic Considerations in Bioreactor Selection and Design

2010 ◽  
Author(s):  
Enes Kadic ◽  
Theodore J. Heindel
Keyword(s):  
Mass Transfer ◽  
Biological Material ◽  
Bubble Column ◽  
Biological Species ◽  
Fluid Mechanic ◽  
Stirred Tank ◽  
Renewable Fuels ◽  
Biological Production ◽  
Fuels And Chemicals ◽  
Liquid Mass Transfer

The biological production of renewable fuels and chemicals, medicines, and proteins is not possible without a properly functioning bioreactor. Bioreactors are expected to meet several basic requirements and create conditions favorable to the biological material such that the desired production is maximized. The basic requirements, which are strongly influenced by fluid mechanic principles, may include minimum damage to the biological material, maximum reactor volume utilization, optimized gas-liquid mass transfer, and/or enhanced mass transfer from the liquid to the biological species. Each of these goals may be achieved within any of the major bioreactor designs, which generally fall under the categories of stirred tank, bubble column, or airlift bioreactor. Yet, each of the bioreactor designs has strengths and weaknesses. This paper provides an overview of bioreactor hydrodynamic developments and the fluid mechanic issues that should to be considered when selecting a bioreactor for experimental and production purposes.

scholarly journals Chimeric cellobiohydrolase I expression, activity, and biochemical properties in three oleaginous yeast

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Markus Alahuhta ◽  
Qi Xu ◽  
Eric P. Knoshaug ◽  
Wei Wang ◽  
Hui Wei ◽  
...  
Keyword(s):  
Corn Stover ◽  
Oleaginous Yeast ◽  
Plant Biomass ◽  
Consolidated Bioprocessing ◽  
Biochemical Properties ◽  
Dilute Acid ◽  
Recombinant Enzymes ◽  
Cellobiohydrolase I ◽  
Pretreated Corn Stover ◽  
Fuels And Chemicals

AbstractConsolidated bioprocessing using oleaginous yeast is a promising modality for the economic conversion of plant biomass to fuels and chemicals. However, yeast are not known to produce effective biomass degrading enzymes naturally and this trait is essential for efficient consolidated bioprocessing. We expressed a chimeric cellobiohydrolase I gene in three different oleaginous, industrially relevant yeast: Yarrowia lipolytica, Lipomyces starkeyi, and Saccharomyces cerevisiae to study the biochemical and catalytic properties and biomass deconstruction potential of these recombinant enzymes. Our results showed differences in glycosylation, surface charge, thermal and proteolytic stability, and efficacy of biomass digestion. L. starkeyi was shown to be an inferior active cellulase producer compared to both the Y. lipolytica and S. cerevisiae enzymes, whereas the cellulase expressed in S. cerevisiae displayed the lowest activity against dilute-acid-pretreated corn stover. Comparatively, the chimeric cellobiohydrolase I enzyme expressed in Y. lipolytica was found to have a lower extent of glycosylation, better protease stability, and higher activity against dilute-acid-pretreated corn stover.

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