Regeneration and characterization of somatic hybrid plants of Citrus sinensis (sweet orange) and Citrus micrantha, a progenitor species of lime

Euphytica ◽  
2004 ◽  
Vol 137 (2) ◽  
pp. 271-278 ◽  
Author(s):  
Iqrar A. Khan ◽  
Jude W. Grosser
Keyword(s):  
Somatic Hybrid ◽  
Citrus Sinensis ◽  
Sweet Orange ◽  
Hybrid Plants

Isolation and Characterization of Copia-like Retrotransposons from 12 Sweet Orange (Citrus sinensis Osbeck) Cultivars

2005 ◽  
Vol 47 (12) ◽  
pp. 1507-1515 ◽  
Author(s):  
Neng-Guo TAO ◽  
Juan XU ◽  
Yun-Jiang CHENG ◽  
Liu HONG ◽  
Wen-Wu GUO ◽  
...  
Keyword(s):  
Citrus Sinensis ◽  
Sweet Orange ◽  
Isolation And Characterization

scholarly journals Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry

2000 ◽  
Vol 23 (3) ◽  
pp. 661-665 ◽  
Author(s):  
Fernanda Januzzi Mendes-da-Glória ◽  
Francisco de Assis Alves Mourão Filho ◽  
Luis Eduardo Aranha Camargo ◽  
Beatriz Madalena Januzzi Mendes
Keyword(s):  
Somatic Hybrid ◽  
Leaf Morphology ◽  
Somatic Hybrids ◽  
Sweet Orange ◽  
Southeastern Brazil ◽  
Citrus Industry ◽  
Rangpur Lime ◽  
Rapd Profile ◽  
Hybrid Plants ◽  
Regenerated Plants

Protoplast culture following polyethylene glycol-induced fusion resulted in the regeneration of somatic hybrid plants between Caipira sweet orange (Citrus sinensis L. Osbeck) and Rangpur lime (C. limonia L. Osbeck). The plants were confirmed as somatic hybrids by leaf morphology, chromosome number and RAPD profile. All regenerated plants were tetraploid (2n = 4x = 36), with intermediate leaf morphology and complementary RAPD banding profile of both parents. This combination may be useful as a rootstock for the citrus industry in Southeastern Brazil since this somatic hybrid could combine the drought tolerance and vigor of Rangpur lime with the blight tolerance of Caipira sweet orange.

scholarly journals Genome Wide Characterization of Short Tandem Repeat Markers in Sweet Orange (Citrus sinensis)

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e104182 ◽  
Author(s):  
Manosh Kumar Biswas ◽  
Qiang Xu ◽  
Christoph Mayer ◽  
Xiuxin Deng
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Citrus Sinensis ◽  
Sweet Orange ◽  
Genome Wide ◽  
Short Tandem Repeat Markers ◽  
Short Tandem

Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata

1985 ◽  
Vol 71 (1) ◽  
pp. 1-4 ◽  
Author(s):  
T. Ohgawara ◽  
S. Kobayashi ◽  
E. Ohgawara ◽  
H. Uchimiya ◽  
S. Ishii
Keyword(s):  
Protoplast Fusion ◽  
Somatic Hybrid ◽  
Citrus Sinensis ◽  
Poncirus Trifoliata ◽  
Hybrid Plants

scholarly journals Production of Tetraploid Somatic Hybrid Breeding Parents for Use in Lemon Cultivar Improvement

HortScience ◽  
1992 ◽  
Vol 27 (5) ◽  
pp. 445-447 ◽  
Author(s):  
N. Tusa ◽  
J.W. Grosser ◽  
F.G. Gmitter ◽  
E.S. Louzada
Keyword(s):  
Somatic Hybrid ◽  
Somatic Hybrids ◽  
Sweet Orange ◽  
Callus Cultures ◽  
Hybrid Breeding ◽  
Root Tip ◽  
Phosphogluconate Dehydrogenase ◽  
Hybrid Plants ◽  
Seedling Leaf ◽  
Cell Chromosome

Allotetraploid somatic hybrid plants of `Hamlin' sweet orange (Citrus sinensis L. Osbeck) + `Femminello' lemon (C. limon L. Burm. f.), and Milam lemon (purported hybrid of C. jambhiri Lush) + `Femminello' lemon were regenerated via somatic embryogenesis following protoplast fusion. `Hamlin' and Milam protoplasts were isolated from undeveloped ovule-derived embryogenic callus cultures and fused using a polyethylene glycol method with seedling leaf-derived protoplasts of `Femminello' lemon. Somatic hybrids were identified on the basis of leaf morphology, root-tip cell chromosome number, and electrophoretic analyses of phosphoglucose isomerase, phosphoglucose mutase, and 6-phosphogluconate dehydrogenase leaf isozymes. The somatic hybrids will be used in interploid crosses with lemon in an effort to generate seedless triploid lemon types with improved tolerance to mal secco disease.

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