Regeneration and characterization of somatic hybrid plants of Citrus sinensis (sweet orange) and Citrus micrantha, a progenitor species of lime

Euphytica ◽  
2004 ◽  
Vol 137 (2) ◽  
pp. 271-278 ◽  
Author(s):  
Iqrar A. Khan ◽  
Jude W. Grosser
2005 ◽  
Vol 47 (12) ◽  
pp. 1507-1515 ◽  
Author(s):  
Neng-Guo TAO ◽  
Juan XU ◽  
Yun-Jiang CHENG ◽  
Liu HONG ◽  
Wen-Wu GUO ◽  
...  

2000 ◽  
Vol 23 (3) ◽  
pp. 661-665 ◽  
Author(s):  
Fernanda Januzzi Mendes-da-Glória ◽  
Francisco de Assis Alves Mourão Filho ◽  
Luis Eduardo Aranha Camargo ◽  
Beatriz Madalena Januzzi Mendes

Protoplast culture following polyethylene glycol-induced fusion resulted in the regeneration of somatic hybrid plants between Caipira sweet orange (Citrus sinensis L. Osbeck) and Rangpur lime (C. limonia L. Osbeck). The plants were confirmed as somatic hybrids by leaf morphology, chromosome number and RAPD profile. All regenerated plants were tetraploid (2n = 4x = 36), with intermediate leaf morphology and complementary RAPD banding profile of both parents. This combination may be useful as a rootstock for the citrus industry in Southeastern Brazil since this somatic hybrid could combine the drought tolerance and vigor of Rangpur lime with the blight tolerance of Caipira sweet orange.


PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e104182 ◽  
Author(s):  
Manosh Kumar Biswas ◽  
Qiang Xu ◽  
Christoph Mayer ◽  
Xiuxin Deng

1985 ◽  
Vol 71 (1) ◽  
pp. 1-4 ◽  
Author(s):  
T. Ohgawara ◽  
S. Kobayashi ◽  
E. Ohgawara ◽  
H. Uchimiya ◽  
S. Ishii

HortScience ◽  
1992 ◽  
Vol 27 (5) ◽  
pp. 445-447 ◽  
Author(s):  
N. Tusa ◽  
J.W. Grosser ◽  
F.G. Gmitter ◽  
E.S. Louzada

Allotetraploid somatic hybrid plants of `Hamlin' sweet orange (Citrus sinensis L. Osbeck) + `Femminello' lemon (C. limon L. Burm. f.), and Milam lemon (purported hybrid of C. jambhiri Lush) + `Femminello' lemon were regenerated via somatic embryogenesis following protoplast fusion. `Hamlin' and Milam protoplasts were isolated from undeveloped ovule-derived embryogenic callus cultures and fused using a polyethylene glycol method with seedling leaf-derived protoplasts of `Femminello' lemon. Somatic hybrids were identified on the basis of leaf morphology, root-tip cell chromosome number, and electrophoretic analyses of phosphoglucose isomerase, phosphoglucose mutase, and 6-phosphogluconate dehydrogenase leaf isozymes. The somatic hybrids will be used in interploid crosses with lemon in an effort to generate seedless triploid lemon types with improved tolerance to mal secco disease.


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