Comparison of ELISA for tissue transglutaminase autoantibodies with antiendomysium antibodies in pediatric and adult patients with celiac disease

The increasing prevalence of celiac disease (CD), especially in adults, its atypical clinical presentation, and the strict, lifelong adherence to gluten-free diet (GFD) as the only option for healthy state create an imperative need for noninvasive methods that can effectively diagnose CD and monitor GFD.Aim. Evaluation of anti-endomysium (EmA) and anti-tissue transglutaminase IgA (tTG-A) antibodies in CD diagnosis, GFD monitoring, and first degree relatives screening in CD adult patients.Methods. 70 newly diagnosed Greek adult patients, 70 controls, and 47 first degree relatives were tested for the presence of EmA and tTG-A. The CD patients were monitored during a 3-year period.Results. EmA predictive ability for CD diagnosis was slightly better compared to tTG-A (P=0.043). EmA could assess compliance with GFD already from the beginning of the diet, while both EmA and tTG-A had an equal ability to discriminate between strictly and partially compliant patients after the first semester and so on. Screening of first degree relatives resulted in the identification of 2 undiagnosed CD cases.Conclusions. Both EmA and tTG-A are suitable markers in the CD diagnosis, in the screening of CD among first degree relatives, having also an equal performance in the long term monitoring.

Download Full-text

Prevalence of celiac disease in adult patients with iron deficiency anemia of obscure origin

QJM ◽

10.1093/qjmed/hcab100.053 ◽

2021 ◽

Vol 114 (Supplement_1) ◽

Author(s):

Mohamed N Farris ◽

Rasha Y Shaheen ◽

Mai A Youssef El-Deeb ◽

Eman A Ebraheem

Keyword(s):

Celiac Disease ◽

Iron Deficiency ◽

Iron Deficiency Anemia ◽

Tissue Transglutaminase ◽

Transferrin Saturation ◽

Duodenal Biopsy ◽

Adult Patients ◽

Deficiency Anemia ◽

Positive Serology ◽

Obscure Origin

Abstract Background Celiac disease is an autoimmune disease which is underestimated over the world. Iron deficiency anemia can be the only presentable symptom for patients with celiac disease. Screening of patients with iron deficiency anemia of obscure origin by anti-tissue transglutaminase serum igA to diagnose celiac disease followed by upper GIT endoscopy is an important step. Objective to evaluate the prevalence of celiac disease in adult patients with iron-deficiency anemia of obscure origin. Methods The present study was a cross-sectional study which included 100 patients with a diagnosis of iron deficiency anemia recruited from Ain Shams University hospitals. All the patients were subjected to: Full history of gastrointestinal symptoms of celiac disease(CD), Age of onset of iron deficiency anemia, Complete blood picture, Serum iron, serum ferritin, transferrin saturation, Anti-tissue transglutaminase antibody immunoglobuline A, Upper gastrointestinal endoscopy and duodenal biopsy to patients who had positive serology. Results The present study showed that 8% of cases with iron deficiency anemia of obscure origin were ultimately diagnosed as cases of celiac diseases while 47% were diagnosed according to duodenal biopsy as potential celiac disease (where there was positive serology and intact villous architecture according to marsh classification) and 45%of cases were non-celiac disease. Conclusion Screening for celiac disease should be considered in patients with iron deficiency anemia of obscure origin

Download Full-text