Immunological evaluation of Escherichia coli -derived hepatitis C virus second envelope protein (E2) variants

2001 ◽  
Vol 58 (3) ◽  
pp. 221-228 ◽  
Author(s):  
S. Dueñas-Carrera ◽  
A. Viña ◽  
H.E. Garay ◽  
O. Reyes ◽  
L. Alvarez-Lajonchere ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Envelope Protein ◽  
Immunological Evaluation

Expression and immunological evaluation of the Escherichia coli-derived hepatitis C virus envelope E1 protein

2000 ◽  
Vol 32 (2) ◽  
pp. 137 ◽  
Author(s):  
Lázaro J. Lorenzo ◽  
Odalys García ◽  
Nelson Acosta-Rivero ◽  
Santiago Dueñas-Carrera ◽  
Gillian Martínez ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Envelope ◽  
E1 Protein ◽  
Immunological Evaluation

Antigenicity and B-epitope mapping of hepatitis C virus envelope protein E2

2009 ◽  
Vol 3 (2) ◽  
pp. 177-182 ◽  
Author(s):  
T. I. Kuzmina ◽  
L. V. Olenina ◽  
M. A. Sanzhakov ◽  
T. E. Farafonova ◽  
T. V. Abramihina ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Envelope Protein ◽  
Epitope Mapping ◽  
Virus Envelope ◽  
Virus Envelope Protein

Canola oilseed‐ and Escherichia coli‐ derived hepatitis C virus (HCV) core proteins adjuvanted with oil bodies, induced robust Th1‐oriented immune responses in immunized mice

Apmis ◽  
2020 ◽  
Vol 128 (11) ◽  
pp. 593-602
Author(s):  
Sara Mohammadzadeh ◽  
Farzin Roohvand ◽  
Parastoo Ehsani ◽  
Ali Hatef Salmanian ◽  
Soheila Ajdary
Keyword(s):  
Escherichia Coli ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Immune Responses ◽  
Oil Bodies ◽  
Core Proteins

scholarly journals Monoclonal Antibody AP33 Defines a Broadly Neutralizing Epitope on the Hepatitis C Virus E2 Envelope Glycoprotein

2005 ◽  
Vol 79 (17) ◽  
pp. 11095-11104 ◽  
Author(s):  
Ania Owsianka ◽  
Alexander W. Tarr ◽  
Vicky S. Juttla ◽  
Dimitri Lavillette ◽  
Birke Bartosch ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Envelope Glycoprotein ◽  
Envelope Protein ◽  
Hypervariable Region ◽  
Neutralizing Antibody ◽  
Broadly Neutralizing Antibody ◽  
New Treatments ◽  
Potent Neutralization

ABSTRACT Hepatitis C virus (HCV) remains a significant threat to the general health of the world's population, and there is a pressing need for the development of new treatments and preventative vaccines. Here, we describe the generation of retrovirus-based pseudoparticles (HCVpp) incorporating a panel of full-length E1E2 clones representative of the major genotypes 1 through 6, and their application to assess the reactivity and neutralizing capability of antisera and monoclonal antibodies raised against portions of the HCV E2 envelope protein. Rabbit antisera raised against either the first hypervariable region or ectodomain of E2 showed limited and strain specific neutralization. By contrast, the monoclonal antibody (MAb) AP33 demonstrated potent neutralization of infectivity against HCVpp carrying E1E2 representative of all genotypes tested. The concentration of AP33 required to achieve 50% inhibition of infection by HCVpp of diverse genotypes ranged from 0.6 to 32 μg/ml. The epitope recognized by MAb AP33 is linear and highly conserved across different genotypes of HCV. Thus, identification of a broadly neutralizing antibody that recognizes a linear epitope is likely to be of significant benefit to future vaccine and therapeutic antibody development.

Purification and characterization of hepatitis C virus non-structural protein 5A expressed in Escherichia coli

2004 ◽  
Vol 37 (1) ◽  
pp. 144-153 ◽  
Author(s):  
Luyun Huang ◽  
Elena V Sineva ◽  
Michele R.S Hargittai ◽  
Suresh D Sharma ◽  
Mehul Suthar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Structural Protein ◽  
Purification And Characterization
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