Root growth and statocyte polarity in lentil seedling roots grown in microgravity or on a slowly rotating clinostat

1990 ◽  
Vol 78 (4) ◽  
pp. 532-537 ◽  
Author(s):  
Giovanna Lorenzi ◽  
Gerald Perbal
1987 ◽  
Vol 14 (6) ◽  
pp. 633 ◽  
Author(s):  
A Bacic ◽  
SF Moody ◽  
JA Mccomb ◽  
JM Hinch ◽  
AE Clarke

Callus initiated from root and embryo of Zea mays proliferated largely through aberrant root growth. In shaken liquid cultures (liquid cultures), the callus remained as clumps of roots but some cells were released into the medium. The extracellular polysaccharides (ECPS) from these culture supernatants are similar with respect to monosaccharide and glycosyl linkage composition to the slime secreted by roots of young Zea mays seedlings but are distinguished from ECPS produced by suspension cultured cell lines of other species by their high fucose content. Callus initiated from root and embryo therefore provides a model for studies of fucosyl-containing polysaccharides secreted by seedling roots.


1989 ◽  
Vol 75 (4) ◽  
pp. 518-524 ◽  
Author(s):  
Gerald Perbal ◽  
Dominique Driss-Ecole

2001 ◽  
Vol 13 (1) ◽  
pp. 13-23
Author(s):  
CRISTIANE ELIZABETH COSTA DE MACÊDO ◽  
JEAN MARIE KINET ◽  
STANLEY LUTTS

Citric and malic acid excretion in the medium and malic acid accumulation in seedling roots and embryo-derived calli as possible mechanisms of aluminum (Al) resistance and the effects of a 17-h Al stress period on root growth in Oryza sativa have been studied. Four-day-old seedlings and embryo-derived calli of Al-resistant (IRAT 112 and IR6023) and Al-sensitive (Aiwu and IKP) cultivars were treated with 250 and 500 µM {Al2(S04)3.18H20 }of total aluminum or without Al for 36 hours. After 3 to 36 hours of stress, seedlings and calli were removed from the flasks and concentration of citric and malic acids was estimated in the Al and control solutions. Malic acid was also assayed in roots tips and in callus tissues. After 17-h of Al stress, inhibition of root growth was a typical effect of Al in rice and the extent of the inhibition depended on both cultivar and Al concentration. At 500 µM of Al, strong reduction of root elongation occurred in all cultivars while at 250 µM of Al, only IRAT was unaffected, when compared to their control. In the absence of Al, all varieties excreted comparable amounts of citric and malic acid. Al treatments, were without effect upon citrate excretion in both Al-resistant and Al-sensitive cultivars. Al treatment, for periods from 3 to 24h, slightly stimulated the excretion of malic acid from seedlings, in all cultivars. Malic acid concentrations in root apices, in the presence or absence of aluminum, were not correlated with aluminum resistance. No differences in malic excretion and internal concentrations were detected between Al-treated and untreated rice calli of the same four cultivars. It is therefore concluded that, in our experimental conditions, differences in Al resistance in our rice cultivars cannot be attributed to citric and malic acids. Further research needs to be carried out to examine other possible mechanisms of Al-resistance in rice and to determine whether organic acids such as succinic and oxalic acid are implicated.


HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 500A-500
Author(s):  
Mustafa Ozden ◽  
Paul H. Jennings

The effects of calcium salts and concentrations from 25 to 200 mM on the induction of chilling tolerance in cucumber roots were studied using total root growth, electrolyte leakage, lipid peroxidation, and activities of antioxidant enzymes as indicies of chilling injury. Cucumber seeds `Poinsett 76' germinated at 25 °C for 36 h were treated with calcium sulfate, calcium nitrate, and calcium chloride for 2 h at 25 °C. After incubation, treated seedlings were rinsed with distilled H2O and chilled at 2 °C for 72 or 96 h with or without re-warming at 25 °C. Roots of CaSO4-treated cucumber seedlings exhibited less chilling injury at all concentrations, when exposed to 72- or 96-h chilling periods with a 72-h re-warming period as shown by greater root growth compared to the chilled control. Concentrations of CaCl2 and Ca(NO3)2 above 100 mM resulted in significant root growth inhibition. Electrolyte leakage (EL) was significantly reduced by CaSO4 up to 150 mM under chilling conditions and all calcium salt treatments reduced malondialdehyde (MDA) levels in seedling roots up to 150 mM. However, at 150 mM CaSO4 both EL and MDA values of 72 h chilled and re-warmed roots were at their lowest levels compared to the control and other treatments. Both superoxide dismutase and catalase activities of seedling roots decreased under chilling conditions compared to the nonchilled control, although the reduction was less in the presence of CaSO4. Peroxidase and glutathione reductase activities increased under chilling conditions and were generally reduced in the presence of calcium salts compared to the chilled control.


1978 ◽  
Vol 26 (5) ◽  
pp. 643 ◽  
Author(s):  
DM Halsall

Zoospores of Phytophthora cinnamomi Rands were attracted, in almost equal numbers, to seedling roots of Eucalyptus sieberi (a susceptible species) and Eucalyptus maculata (a field resistant species). Germinating zoospores penetrated and mycelium developed within seedling roots of both species. The root and shoot growth of both eucalypt species were reduced by P. cinnamomi infection. In E. sieberi the growth reduction was greater than in E. maculata seedlings and other symptoms of infection were evident. Cell-free extracts of P. cinnamomi were able to produce some of the symptoms of infection in the absence of parasitism. Root growth of both eucalypt species was reduced at high extract dilutions and seedling deaths occurred at low extract dilutions.


Plant Biology ◽  
2011 ◽  
Vol 14 (3) ◽  
pp. 467-474 ◽  
Author(s):  
A. V. Vollsnes ◽  
T. B. Melø ◽  
C. M. Futsaether

Author(s):  
Jae Hwan Kim ◽  
Byoung-Youn Kang ◽  
Jungho Ryu ◽  
In-Hyun Nam

The purpose of this study was to determine the effects that different container conditions have on Pinus densiflora seedling growth. Under greenhouse cultivation, there were no statistical differences observed in plant height and the number of branches; however, significant differences in root collar diameter and root status were observed. In control container growth conditions, the roots grew in an abnormal spiral shape, while in negative phototropism container growth conditions the roots grew in a vertical shape. In outdoor cultivation, seedlings in various container growth conditions showed significant differences. The seedlings that were grown in negative phototropism container growth conditions showed the greatest increases in height, number of branches, root collar diameter, and root growth. This study determined that seedling roots in negative phototropism container growth conditions grew vertically, thus displaying successful rooting when they were transplanted outdoors. This resulted in favorable measurements in height, number of branches, root collar diameter, and root growth.


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