Identification of a single-copy gene encoding a Type I chlorophyll a/b-binding polypeptide of photosystem I in Arabidopsis thaliana

1992 ◽  
Vol 84 (4) ◽  
pp. 561-567 ◽  
Author(s):  
Poul E. Jensen ◽  
Michael Kristensen ◽  
Tine Hoff ◽  
Jan Lehmbeck ◽  
Bjarne M. Stummann ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Chlorophyll A ◽  
Photosystem I ◽  
Single Copy ◽  
Type I ◽  
Single Copy Gene ◽  
Gene Encoding ◽  
Copy Gene

Identification of a single-copy gene encoding a Type I chlorophyll a/b-binding polypeptide of photosystem I in Arabidopsis thaliana

1992 ◽  
Vol 84 (4) ◽  
pp. 561-567 ◽  
Author(s):  
Poul E. Jensen ◽  
Michael Kristensen ◽  
Tine Hoff ◽  
Jan Lehmbeck ◽  
Bjarne M. Stummann ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Chlorophyll A ◽  
Photosystem I ◽  
Single Copy ◽  
Type I ◽  
Single Copy Gene ◽  
Gene Encoding ◽  
Copy Gene

scholarly journals A Single-Copy Gene Encodes Kex1, a Serine Endoprotease of Pneumocystis jiroveci

2003 ◽  
Vol 71 (1) ◽  
pp. 571-574 ◽  
Author(s):  
Geetha Kutty ◽  
Joseph A. Kovacs
Keyword(s):  
Golgi Apparatus ◽  
Pneumocystis Carinii ◽  
Single Copy ◽  
Single Copy Gene ◽  
Gene Encoding ◽  
Pneumocystis Jiroveci ◽  
Copy Gene ◽  
Biological Differences

ABSTRACT We have cloned and characterized the kex1 gene of Pneumocystis jiroveci. Unlike the case for Pneumocystis carinii, in which the homologous PRT-1 genes are multicopy, kex1 is a single-copy gene encoding a protein homologous to fungal serine endoproteases, which localize to the Golgi apparatus. Thus, substantial biological differences can be seen among Pneumocystis species.

Dictyostelium discoideum contains a single-copy gene encoding a unique subtype of histone H1

Gene ◽  
1995 ◽  
Vol 154 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Loren J. Hauser ◽  
Madhu S. Dhar ◽  
Donald E. Olins
Keyword(s):  
Dictyostelium Discoideum ◽  
Single Copy ◽  
Histone H1 ◽  
Single Copy Gene ◽  
Gene Encoding ◽  
Copy Gene

scholarly journals Characterization of a single copy gene encoding ferredoxin I from pea.

1989 ◽  
Vol 1 (7) ◽  
pp. 681-690 ◽  
Author(s):  
R C Elliott ◽  
T J Pedersen ◽  
B Fristensky ◽  
M J White ◽  
L F Dickey ◽  
...  
Keyword(s):  
Single Copy ◽  
Single Copy Gene ◽  
Gene Encoding ◽  
Copy Gene ◽  
Ferredoxin I

scholarly journals An Allelic Series of Blue Fluorescent trp1 Mutants of Arabidopsis thaliana

Genetics ◽  
1997 ◽  
Vol 145 (1) ◽  
pp. 197-205 ◽  
Author(s):  
Alan B Rose ◽  
Jiayang Li ◽  
Robert L Last
Keyword(s):  
Arabidopsis Thaliana ◽  
Sequence Divergence ◽  
Single Copy ◽  
Flowering Plant ◽  
Wild Type ◽  
Allelic Series ◽  
Gene Encoding ◽  
Copy Gene ◽  
High Degree ◽  
Plant Arabidopsis Thaliana

Nine blue fluorescent mutants of the flowering plant Arabidopsis thaliana were isolated by genetic selections and fluorescence screens. Each was shown to contain a recessive allele of trp1, a previously described locus that encodes the tryptophan biosynthetic enzyme phosphoribosylanthranilate transferase (PAT, called trpD in bacteria). The trp1 mutants consist of two groups, tryptophan auxotrophs and prototrophs, that differ significantly in growth rate, morphology, and fertility. The trp1 alleles cause plants to accumulate varying amounts of blue fluorescent anthranilate compounds, and only the two least severely affected of the prototrophs have any detectable PAT enzyme activity. All four of the trp1 mutations that were sequenced are G to A or C to T transitions that cause an amino acid change, but in only three of these is the affected residue phylogenetically conserved. There is an unusually high degree of sequence divergence in the single-copy gene encoding PAT from the wild-type Columbia and Landsberg erecta ecotypes of Arabidopsis.

Characterization of the promoter from the single-copy gene encoding ferredoxin-NADP+-oxidoreductase from spinach

1993 ◽  
Vol 237-237 (1-2) ◽  
pp. 261-272 ◽  
Author(s):  
R. Oelmüller ◽  
C. Bolle ◽  
A. K. Tyagi ◽  
N. Niekrawietz ◽  
S. Breit ◽  
...  
Keyword(s):  
Single Copy ◽  
Single Copy Gene ◽  
Gene Encoding ◽  
Copy Gene

Characterization of a Single Copy Gene Encoding Ferredoxin I from Pea

1989 ◽  
Vol 1 (7) ◽  
pp. 681 ◽  
Author(s):  
Robert C. Elliott ◽  
Thomas J. Pedersen ◽  
Brian Fristensky ◽  
Michael J. White ◽  
Lynn F. Dickey ◽  
...  
Keyword(s):  
Single Copy ◽  
Single Copy Gene ◽  
Gene Encoding ◽  
Copy Gene ◽  
Ferredoxin I

A Mouse Single-Copy Gene,Gtf2i,the Homolog of HumanGTF2I,That Is Duplicated in the Williams–Beuren Syndrome Deletion Region

Genomics ◽  
1998 ◽  
Vol 48 (2) ◽  
pp. 163-170 ◽  
Author(s):  
Yu-Ker Wang ◽  
Luis A. Pérez-Jurado ◽  
Uta Francke
Keyword(s):  
Single Copy ◽  
Single Copy Gene ◽  
Deletion Region ◽  
Copy Gene

scholarly journals Real-Time PCR for Molecular Detection of Zoonotic and Non-Zoonotic Giardia spp. in Wild Rodents

2021 ◽  
Vol 9 (8) ◽  
pp. 1610
Author(s):  
Christian Klotz ◽  
Elke Radam ◽  
Sebastian Rausch ◽  
Petra Gosten-Heinrich ◽  
Toni Aebischer
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Gastrointestinal Disease ◽  
Single Copy ◽  
Marker Genes ◽  
Small Rodent ◽  
Analytical Sensitivity ◽  
Single Copy Gene ◽  
Wild Rodents ◽  
Copy Gene

Giardiasis in humans is a gastrointestinal disease transmitted by the potentially zoonotic Giardia duodenalis genotypes (assemblages) A and B. Small wild rodents such as mice and voles are discussed as potential reservoirs for G. duodenalis but are predominantly populated by the two rodent species Giardia microti and Giardia muris. Currently, the detection of zoonotic and non-zoonotic Giardia species and genotypes in these animals relies on cumbersome PCR and sequencing approaches of genetic marker genes. This hampers the risk assessment of potential zoonotic Giardia transmissions by these animals. Here, we provide a workflow based on newly developed real-time PCR schemes targeting the small ribosomal RNA multi-copy gene locus to distinguish G. muris, G. microti and G. duodenalis infections. For the identification of potentially zoonotic G. duodenalis assemblage types A and B, an established protocol targeting the single-copy gene 4E1-HP was used. The assays were specific for the distinct Giardia species or genotypes and revealed an analytical sensitivity of approximately one or below genome equivalent for the multi-copy gene and of about 10 genome equivalents for the single-copy gene. Retesting a biobank of small rodent samples confirmed the specificity. It further identified the underlying Giardia species in four out of 11 samples that could not be typed before by PCR and sequencing. The newly developed workflow has the potential to facilitate the detection of potentially zoonotic and non-zoonotic Giardia species in wild rodents.

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