Suspension Culture of Mammalian Cells and Macromolecular Growth-Promoting Fractions of Calf Serum

Nature ◽  
1964 ◽  
Vol 201 (4917) ◽  
pp. 375-378 ◽  
Author(s):  
B. T. TOZER ◽  
S. J. PIRT
Keyword(s):  
Suspension Culture ◽  
Mammalian Cells ◽  
Calf Serum ◽  
Growth Promoting

Effects of follicular fluids on the growth of porcine preantral follicle and oocyte

Zygote ◽  
2008 ◽  
Vol 16 (3) ◽  
pp. 239-247 ◽  
Author(s):  
T. Metoki ◽  
H. Iwata ◽  
M. Itoh ◽  
M. Kasai ◽  
A. Takajyo ◽  
...  
Keyword(s):  
Calf Serum ◽  
Follicle Development ◽  
Preantral Follicle ◽  
Promoting Effect ◽  
Oocyte Growth ◽  
Preantral Follicles ◽  
Molecular Weights ◽  
Heat Treated ◽  
Growth Promoting ◽  
Follicular Fluids

SummaryWe examined the effect of supplementing the culture medium with follicular fluid (FF) on the growth of porcine preantral follicles and oocytes. Firstly, preantral follicles were retrieved from ovaries and then FF was collected from all antral follicles that were 2–7 mm in diameter (AFF), which included large follicles of 4–7 mm in diameter (LFF) and small follicles of 2–3 mm in diameter (SFF). When preantral follicles with a diameter of 250 μm were cultured in medium containing AFF, the growth of follicles and oocytes was greater than when follicles were cultured in medium containing fetal calf serum (FCS). When this growth-promoting effect in AFF was compared for LFF and SFF, the LFF were shown to be significantly more effective than SFF. This LFF effect was lost, however, when the concentration of LFF in the medium was decreased from 5% to 0.5% or when LFF were heat treated (60 °C for 30 min) or trypsin was added. In contrast, a decrease in SFF concentration from 5% to 0.5% and heat treatment of the SFF enhanced preantral follicle growth. Furthermore, proteins obtained from LFF that had molecular weights greater than 10 kDa (LFF > 10 kDa) had similar, but relatively reduced, growth-promoting properties. The remaining three LFF protein fractions (<10 kDa or <100 kDa or >100 kDa), however, did not have these growth-promoting properties. In conclusion, the supplementation of medium with LFF, rather than serum, enhanced preantral follicle and oocyte growth. Factors that enhanced follicle development in LFF and factors that suppressed follicle development in SFF were proteins and these LFF factors ranged in size from 10 kDa to over 100 kDa.

An Inhibitor Present in Calf Serum Which Prevents Growth of BHK21 Cells in Suspension Culture

1972 ◽  
Vol 10 (1) ◽  
pp. 137-152
Author(s):  
H. OTSUKA
Keyword(s):  
Suspension Culture ◽  
Calf Serum ◽  
Soft Agar ◽  
Transformed Cells ◽  
Ammonium Sulphate Precipitation ◽  
Serum Inhibitor ◽  
Swine Serum ◽  
Rna And Dna Synthesis ◽  
Rna And Dna ◽  
Do So

BHK21 cells do not form colonies in soft agar suspension culture in the presence of calf serum; but if swine serum is used instead they do so. In methylcellulose suspension culture supplemented with calf serum the cells synthesize very little RNA or DNA, but if swine serum is used there is a marked increase of syntheses. Calf serum was found to contain an inhibitor which prevents the induction, but not the continuation, of RNA and DNA synthesis in BHK21 cells. In contrast the growth of polyoma-virus-transformed cells is not affected by this serum inhibitor which has been partially purified from calf serum by ammonium sulphate precipitation followed by Sephadex G-200 column chromatography.

The application of rapid kinetic techniques to the transport of thymidine and 3-O-methylglucose into mammalian cells in suspension culture

1976 ◽  
Vol 89 (4) ◽  
pp. 605-612 ◽  
Author(s):  
Robert M. Wohlhueter ◽  
Richard Marz ◽  
Jon C. Graff ◽  
Peter G. W. Plagemann
Keyword(s):  
Suspension Culture ◽  
Mammalian Cells

scholarly journals Electric pulse-mediated gene transfer in mammalian cells grown in suspension culture.

1986 ◽  
Vol 11 (2) ◽  
pp. 191-197 ◽  
Author(s):  
Hiroko Hama-Inaba ◽  
Tadahiro Shiomi ◽  
Koki Sato ◽  
Akemi Ito ◽  
Michiki Kasai
Keyword(s):  
Gene Transfer ◽  
Suspension Culture ◽  
Mammalian Cells ◽  
Electric Pulse

Production of Recombinant Therapeutic Proteins by Mammalian Cells in Suspension Culture

2005 ◽  
pp. 107-122 ◽  
Author(s):  
Lily Chu ◽  
Ilse Blumentals ◽  
Gargi Maheshwari
Keyword(s):  
Suspension Culture ◽  
Mammalian Cells ◽  
Therapeutic Proteins

scholarly journals Microparticles for Suspension Culture of Mammalian Cells

2019 ◽  
Vol 2 (7) ◽  
pp. 2791-2801 ◽  
Author(s):  
Daniel Smith ◽  
Chase Herman ◽  
Sidharth Razdan ◽  
Muhammad Raisul Abedin ◽  
William Van Stoecker ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Mammalian Cells

Fetal calf serum-free suspension culture of Chinese hamster ovary cells employing fish serum

2010 ◽  
Vol 109 (3) ◽  
pp. 307-309 ◽  
Author(s):  
Masashi Fujiwara ◽  
Yu Aizu ◽  
Itaru Shioya ◽  
Mutsumi Takagi
Keyword(s):  
Suspension Culture ◽  
Chinese Hamster Ovary ◽  
Fetal Calf Serum ◽  
Chinese Hamster Ovary Cells ◽  
Calf Serum ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Serum Free ◽  
Serum Free Suspension ◽  
Fish Serum

scholarly journals THE FATE OF DNA-CONTAINING PARTICLES PHAGOCYTIZED BY MAMMALIAN CELLS

1964 ◽  
Vol 21 (1) ◽  
pp. 105-114 ◽  
Author(s):  
Klaus Bensch ◽  
Gerald Gordon ◽  
Leonard Miller
Keyword(s):  
Acid Phosphatase ◽  
Suspension Culture ◽  
Mammalian Cells ◽  
Complete Hydrolysis ◽  
Hydrolysis Of

Particulate DNA protein coacervates were digested immediately after being phagocytized by L strain fibroblasts in suspension culture. Enlargement of the phagocytotic vacuoles occurred simultaneously with a loss of the electron opacity of the phagocytized particles. Cytochemical reactions positive for non-specific esterase, acid phosphatase, and nucleoside phosphatase in the phagocytotic vacuoles provided additional evidence for the probability of complete hydrolysis of the phagocytized nucleoprotein.

scholarly journals Identification of the Insulin-Like Growth Factor II Receptor as a Novel Receptor for Binding and Invasion by Listeria monocytogenes

2006 ◽  
Vol 74 (1) ◽  
pp. 566-577 ◽  
Author(s):  
Uta Gasanov ◽  
Craig Koina ◽  
Kenneth W. Beagley ◽  
R. John Aitken ◽  
Philip M. Hansbro
Keyword(s):  
Listeria Monocytogenes ◽  
Growth Factor ◽  
Mammalian Cells ◽  
Affinity Purification ◽  
Calf Serum ◽  
Peptide Sequence ◽  
Insulin Like Growth Factor ◽  
Phosphate Binding ◽  
Factor Ii ◽  
Mannose 6 Phosphate

ABSTRACT The gram-positive bacterium Listeria monocytogenes causes a life-threatening disease known as listeriosis. The mechanism by which L. monocytogenes invades mammalian cells is not fully understood, but the processes involved may provide targets to prevent and treat listeriosis. Here, for the first time, we have identified the insulin-like growth factor II receptor (IGFIIR; also known as the cation-independent mannose 6-phosphate receptor CIM6PR or CD222) as a novel receptor for binding and invasion of Listeria species. Random peptide phage display was employed to select a peptide sequence by panning with immobilized L. monocytogenes cells; this peptide sequence corresponds to a sequence within the mannose 6-phosphate binding site of the IGFIIR. All Listeria spp. specifically bound the labeled peptide but not a control peptide, which was demonstrated using fluorescence spectrophotometry and fluorescence-activated cell sorting. Further evidence for binding of the receptor by L. monocytogenes and L. innocua was provided by affinity purification of the bovine IGFIIR from fetal calf serum by use of magnetic beads coated with cell preparations of Listeria spp. as affinity matrices. Adherence to and invasion of mammalian cells by L. monocytogenes was significantly inhibited by both the synthetic peptide and mannose 6-phosphate but not by appropriate controls. These observations indicate a role for the IGFIIR in the adherence and invasion of L. monocytogenes of mammalian cells, perhaps in combination with known mechanisms. Ligation of IGFIIR by L. monocytogenes may be a novel mechanism that contributes to the regulation of infectivity, possibly in combination with other mechanisms.

Gas chromatographic examination of medium composition during growth of mammalian cells in suspension culture

1972 ◽  
Vol 46 (2) ◽  
pp. 421-432 ◽  
Author(s):  
Donald C. Fish ◽  
James P. Dobbs ◽  
Robert L. Sine ◽  
Jerry M. Elliott
Keyword(s):  
Suspension Culture ◽  
Mammalian Cells ◽  
Medium Composition
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