Change in the Template Specificity of RNA Polymerase during Sporulation of Bacillus subtilis

Nature ◽  
1969 ◽  
Vol 224 (5214) ◽  
pp. 35-37 ◽  
Author(s):  
RICHARD LOSICK ◽  
ABRAHAM L. SONENSHEIN
Keyword(s):  
Bacillus Subtilis ◽  
Rna Polymerase ◽  
Template Specificity

scholarly journals The δ subunit and NTPase HelD institute a two-pronged mechanism for RNA polymerase recycling

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Hao-Hong Pei ◽  
Tarek Hilal ◽  
Zhuo A. Chen ◽  
Yong-Heng Huang ◽  
Yuan Gao ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Nucleic Acids ◽  
Rna Polymerase ◽  
Active Site ◽  
Genome Stability ◽  
Slow Growth ◽  
Coiled Coil ◽  
Main Channel ◽  
Dependent Manner ◽  
Secondary Channel

AbstractCellular RNA polymerases (RNAPs) can become trapped on DNA or RNA, threatening genome stability and limiting free enzyme pools, but how RNAP recycling into active states is achieved remains elusive. In Bacillus subtilis, the RNAP δ subunit and NTPase HelD have been implicated in RNAP recycling. We structurally analyzed Bacillus subtilis RNAP-δ-HelD complexes. HelD has two long arms: a Gre cleavage factor-like coiled-coil inserts deep into the RNAP secondary channel, dismantling the active site and displacing RNA, while a unique helical protrusion inserts into the main channel, prying the β and β′ subunits apart and, aided by δ, dislodging DNA. RNAP is recycled when, after releasing trapped nucleic acids, HelD dissociates from the enzyme in an ATP-dependent manner. HelD abundance during slow growth and a dimeric (RNAP-δ-HelD)2 structure that resembles hibernating eukaryotic RNAP I suggest that HelD might also modulate active enzyme pools in response to cellular cues.

scholarly journals DNA-dependent RNA polymerase from vegetative cells and from spores of Bacillus subtilis

FEBS Letters ◽  
1971 ◽  
Vol 13 (5) ◽  
pp. 269-274 ◽  
Author(s):  
J.C.C. Maia ◽  
P. Kerjan ◽  
J. Szulmajster
Keyword(s):  
Bacillus Subtilis ◽  
Rna Polymerase ◽  
Vegetative Cells
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