Selection of high-affinity phage antibodies from phage display libraries

10.1038/7959 ◽  
1999 ◽  
Vol 17 (4) ◽  
pp. 397-399 ◽  
Author(s):  
Robert de Bruin ◽  
Kees Spelt ◽  
Joseph Mol ◽  
Ronald Koes ◽  
Francesca Quattrocchio
Keyword(s):  
Phage Display ◽  
High Affinity ◽  
Phage Antibodies ◽  
Phage Display Libraries ◽  
Selection Of

Phage Display as A Bio-Technique for Cancer Immunotherapy

2020 ◽  
Vol 17 (4) ◽  
pp. 379-387
Author(s):  
Shirin Mahmoodi ◽  
Navid Nezafat ◽  
Younes Ghasemi
Keyword(s):  
Phage Display ◽  
Cancer Immunotherapy ◽  
Tumor Antigens ◽  
High Affinity ◽  
Phage Antibodies ◽  
Phage Display Libraries ◽  
Antigen Presenting ◽  
Immunogenic Properties ◽  
Specific Peptide ◽  
Selection Of

Background: Phage display is a biotechnological technique that presents peptides with coated proteins on the surface of phage. In the last two decades, growing applications of phage display in various fields of biotechnology have been investigated. Phage display libraries allow to present billions of peptides on phage surface for selection of a specific peptide with the desired affinity. Objective: In this regard, high-affinity phage antibodies against tumor antigens are produced and applied for diagnosis and treatment of cancer. Method: Moreover, phage display libraries are employed to select the high affinity T Cell Receptors (TCRs) for the peptide-MHC complex which is an attractive approach in cancer immunotherapy. Due to immunogenic properties of phage particles, phage-based vaccines do not require adjuvant, in addition the phage particles can effectively take up by Antigen Presenting Cells (APCs). Results: Taken together, phage-based cancer vaccines are ideal candidates that provide a key for eradication of tumor cells. Conclusion: In this review, we focus on various applications of a phage display platform in different types of cancer immunotherapy approaches.

Combination of ribosome and phage display for fast selection of high affinity VHH antibody fragments

2019 ◽  
pp. 27-33
Author(s):  
YuE Kravchenko ◽  
SV Ivanov ◽  
DS Kravchenko ◽  
EI Frolova ◽  
SP Chumakov
Keyword(s):  
Phage Display ◽  
Selection Procedure ◽  
Free System ◽  
Phage Displays ◽  
High Affinity ◽  
Binding Domains ◽  
A Cell ◽  
Vhh Antibodies ◽  
Efficiency Of Selection ◽  
Selection Of

Selection of antibodies using phage display involves the preliminary cloning of the repertoire of sequences encoding antigen-binding domains into phagemid, which is considered the bottleneck of the method, limiting the resulting diversity of libraries and leading to the loss of poorly represented variants before the start of the selection procedure. Selection in cell-free conditions using a ribosomal display is devoid from this drawback, however is highly sensitive to PCR artifacts and the RNase contamination. The aim of the study was to test the efficiency of a combination of both methods, including pre-selection in a cell-free system to enrich the source library, followed by cloning and final selection using phage display. This approach may eliminate the shortcomings of each method and increase the efficiency of selection. For selection, alpaca VHH antibody sequences suitable for building an immune library were used due to the lack of VL domains. Analysis of immune libraries from the genes of the VH3, VHH3 and VH4 families showed that the VHH antibodies share in the VH3 and VH4 gene groups is insignificant, and selection from the combined library is less effective than from the VHH3 family of sequences. We found that the combination of ribosomal and phage displays leads to a higher enrichment of high-affinity fragments and avoids the loss of the original diversity during cloning. The combined method allowed us to obtain a greater number of different high-affinity sequences, and all the tested VHH fragments were able to specifically recognize the target, including the total protein extracts of cell cultures.

scholarly journals Selection of Peptides Targeting Helix 31 of Bacterial 16S Ribosomal RNA by Screening M13 Phage-Display Libraries

Molecules ◽  
2011 ◽  
Vol 16 (2) ◽  
pp. 1211-1239 ◽  
Author(s):  
Tek N. Lamichhane ◽  
N. Dinuka Abeydeera ◽  
Anne-Cécile E. Duc ◽  
Philip R. Cunningham ◽  
Christine S. Chow
Keyword(s):  
Phage Display ◽  
Ribosomal Rna ◽  
16S Ribosomal Rna ◽  
Phage Display Libraries ◽  
M13 Phage ◽  
Selection Of

[29] Design and use of phage display libraries for the selection of antibodies and enzymes

2000 ◽  
pp. 480-505 ◽  
Author(s):  
Francesca Viti ◽  
Fredrik Nilsson ◽  
Salvatore Demartis ◽  
Adrian Huber ◽  
Dario Neri
Keyword(s):  
Phage Display ◽  
Phage Display Libraries ◽  
Selection Of

87 Selection of pre-invasive lung cancer binding peptides using random phage display libraries

2004 ◽  
Vol 2 (8) ◽  
pp. 29
Author(s):  
J.Y. Hung ◽  
A.J. Rebello ◽  
S. Lam ◽  
J.C. Ieriche
Keyword(s):  
Lung Cancer ◽  
Phage Display ◽  
Phage Display Libraries ◽  
Binding Peptides ◽  
Selection Of

The role of valency in the selection of anti-carbohydrate single-chain Fvs from phage display libraries

1998 ◽  
Vol 220 (1-2) ◽  
pp. 39-49 ◽  
Author(s):  
Roger MacKenzie ◽  
Rebecca To
Keyword(s):  
Phage Display ◽  
Single Chain ◽  
Phage Display Libraries ◽  
Selection Of

scholarly journals Selection of high affinity human neutralizing antibodies to VEGFR2 from a large antibody phage display library for antiangiogenesis therapy

2001 ◽  
Vol 97 (3) ◽  
pp. 393-399 ◽  
Author(s):  
Dan Lu ◽  
Xenia Jimenez ◽  
Haifan Zhang ◽  
Peter Bohlen ◽  
Larry Witte ◽  
...  
Keyword(s):  
Phage Display ◽  
Neutralizing Antibodies ◽  
Phage Display Library ◽  
High Affinity ◽  
Antibody Phage ◽  
Antibody Phage Display ◽  
Antiangiogenesis Therapy ◽  
Selection Of
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