scholarly journals Simvastatin and Atorvastatin inhibit DNA replication licensing factor MCM7 and effectively suppress RB-deficient tumors growth

2017 ◽  
Vol 8 (3) ◽  
pp. e2673-e2673 ◽  
Author(s):  
Juan Li ◽  
Jie Liu ◽  
Zheyong Liang ◽  
Fang He ◽  
Lu Yang ◽  
...  
Keyword(s):  
Dna Replication ◽  
Licensing Factor

A murine replication protein accumulates temporarily in the heterochromatic regions of nuclei prior to initiation of DNA replication

1995 ◽  
Vol 108 (3) ◽  
pp. 927-934 ◽  
Author(s):  
M. Starborg ◽  
E. Brundell ◽  
K. Gell ◽  
C. Larsson ◽  
I. White ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dna Replication ◽  
Mammalian Cells ◽  
Mitotic Cell ◽  
Yeast Cells ◽  
Metaphase Chromosomes ◽  
Licensing Factor ◽  
Mammalian Homologue ◽  
Initiation Of Dna Replication

We have analyzed the expression of the murine P1 gene, the mammalian homologue of the yeast MCM3 protein, during the mitotic cell cycle. The MCM3 protein has previously been shown to be of importance for initiation of DNA replication in Saccharomyces cerevisiae. We found that the murine P1 protein was present in the nuclei of mammalian cells throughout interphase of the cell cycle. This is in contrast to the MCM3 protein, which is located in the nuclei of yeast cells only between the M and the S phase of the cell cycle. Detailed analysis of the intranuclear localization of the P1 protein during the cell cycle revealed that it accumulates transiently in the heterochromatic regions towards the end of G1. The accumulation of the P1 protein in the heterochromatic regions prior to activation of DNA replication suggests that the mammalian P1 protein is also of importance for initiation of DNA replication. The MCM2-3.5 proteins have been suggested to represent yeast equivalents of a hypothetical replication licensing factor initially described in Xenopus. Our data support this model and indicate that the murine P1 protein could function as replication licensing factor. The chromosomal localization of the P1 gene was determined by fluorescence in situ hybridization to region 6p12 in human metaphase chromosomes.

DNA replication licensing factor

1996 ◽  
pp. 83-90 ◽  
Author(s):  
James P. J. Chong ◽  
J. Julian Blow
Keyword(s):  
Dna Replication ◽  
Licensing Factor

scholarly journals Proteolysis of DNA Replication Licensing Factor Cdt1 in S-phase Is Performed Independently of Geminin through Its N-terminal Region

2004 ◽  
Vol 279 (29) ◽  
pp. 30807-30816 ◽  
Author(s):  
Hideo Nishitani ◽  
Zoi Lygerou ◽  
Takeharu Nishimoto
Keyword(s):  
Dna Replication ◽  
S Phase ◽  
Terminal Region ◽  
Licensing Factor

scholarly journals DNA Replication Licensing Factor Minichromosome Maintenance Deficient 5 Rescues p53-Mediated Growth Arrest

2007 ◽  
Vol 67 (1) ◽  
pp. 116-121 ◽  
Author(s):  
Mukesh K. Agarwal ◽  
A.R.M. R. Amin ◽  
Munna L. Agarwal
Keyword(s):  
Dna Replication ◽  
Growth Arrest ◽  
Minichromosome Maintenance ◽  
Licensing Factor

scholarly journals Acetylation/Deacetylation Modulates the Stability of DNA Replication Licensing Factor Cdt1

2009 ◽  
Vol 284 (17) ◽  
pp. 11446-11453 ◽  
Author(s):  
Michele A. Glozak ◽  
Edward Seto
Keyword(s):  
Dna Replication ◽  
Licensing Factor ◽  
The Stability

scholarly journals Negative Regulation of DNA Replication by the Retinoblastoma Protein Is Mediated by Its Association with MCM7

1998 ◽  
Vol 18 (5) ◽  
pp. 2748-2757 ◽  
Author(s):  
Jacqueline M. Sterner ◽  
Susan Dew-Knight ◽  
Christine Musahl ◽  
Sally Kornbluth ◽  
Jonathan M. Horowitz
Keyword(s):  
Amino Acids ◽  
Dna Replication ◽  
Protein Complexes ◽  
Replication Assay ◽  
Amino Terminal ◽  
Licensing Factor ◽  
Human Proteins ◽  
Carboxy Terminal ◽  
Related Proteins

ABSTRACT A yeast two-hybrid screen was employed to identify human proteins that specifically bind the amino-terminal 400 amino acids of the retinoblastoma (Rb) protein. Two independent cDNAs resulting from this screen were found to encode the carboxy-terminal 137 amino acids of MCM7, a member of a family of proteins that comprise replication licensing factor. Full-length Rb and MCM7 form protein complexes in vitro, and the amino termini of two Rb-related proteins, p107 and p130, also bind MCM7. Protein complexes between Rb and MCM7 were also detected in anti-Rb immunoprecipitates prepared from human cells. The amino-termini of Rb and p130 strongly inhibited DNA replication in an MCM7-dependent fashion in a Xenopus in vitro DNA replication assay system. These data provide the first evidence that Rb and Rb-related proteins can directly regulate DNA replication and that components of licensing factor are targets of the products of tumor suppressor genes.

scholarly journals The Human Licensing Factor for DNA Replication Cdt1 Accumulates in G1and Is Destabilized after Initiation of S-phase

2001 ◽  
Vol 276 (48) ◽  
pp. 44905-44911 ◽  
Author(s):  
Hideo Nishitani ◽  
Stavros Taraviras ◽  
Zoi Lygerou ◽  
Takeharu Nishimoto
Keyword(s):  
Dna Replication ◽  
S Phase ◽  
Licensing Factor
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