scholarly journals An evaluation of the inflammatory response of lipopolysaccharide-treated primary dental pulp cells with regard to calcium silicate-based cements

2014 ◽  
Vol 6 (2) ◽  
pp. 94-98 ◽  
Author(s):  
Wei-Yun Lai ◽  
Chia-Tze Kao ◽  
Chi-Jr Hung ◽  
Tsui-Hsien Huang ◽  
Ming-You Shie
2015 ◽  
Vol 23 (5) ◽  
pp. 467-471 ◽  
Author(s):  
Leticia Boldrin MESTIERI ◽  
Ana Lívia GOMES-CORNÉLIO ◽  
Elisandra Márcia RODRIGUES ◽  
Loise Pedrosa SALLES ◽  
Roberta BOSSO-MARTELO ◽  
...  

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Sung-Chih Hsieh ◽  
Jeng-Ting Tsao ◽  
Wei-Zhen Lew ◽  
Ya-Hui Chan ◽  
Lin-Wen Lee ◽  
...  

One of the causes of dental pulpitis is lipopolysaccharide- (LPS-) induced inflammatory response. Following pulp tissue inflammation, odontoblasts, dental pulp cells (DPCs), and dental pulp stem cells (DPSCs) will activate and repair damaged tissue to maintain homeostasis. However, when LPS infection is too serious, dental repair is impossible and disease may progress to irreversible pulpitis. Therefore, the aim of this study was to examine whether static magnetic field (SMF) can attenuate inflammatory response of dental pulp cells challenged with LPS. In methodology, dental pulp cells were isolated from extracted teeth. The population of DPSCs in the cultured DPCs was identified by phenotypes and multilineage differentiation. The effects of 0.4 T SMF on DPCs were observed through MTT assay and fluorescent anisotropy assay. Our results showed that the SMF exposure had no effect on surface markers or multilineage differentiation capability. However, SMF exposure increases cell viability by 15%. In addition, SMF increased cell membrane rigidity which is directly related to higher fluorescent anisotropy. In the LPS-challenged condition, DPCs treated with SMF demonstrated a higher tolerance to LPS-induced inflammatory response when compared to untreated controls. According to these results, we suggest that 0.4 T SMF attenuates LPS-induced inflammatory response to DPCs by changing cell membrane stability.


2018 ◽  
Vol 27 (11) ◽  
pp. 1541-1547 ◽  
Author(s):  
Emel Onay ◽  
Erkan Yurtcu ◽  
Yunus Terzi ◽  
Mete Ungor ◽  
Yener Oguz ◽  
...  

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