Static Magnetic Field Attenuates Lipopolysaccharide-Induced Inflammation in Pulp Cells by Affecting Cell Membrane Stability

One of the causes of dental pulpitis is lipopolysaccharide- (LPS-) induced inflammatory response. Following pulp tissue inflammation, odontoblasts, dental pulp cells (DPCs), and dental pulp stem cells (DPSCs) will activate and repair damaged tissue to maintain homeostasis. However, when LPS infection is too serious, dental repair is impossible and disease may progress to irreversible pulpitis. Therefore, the aim of this study was to examine whether static magnetic field (SMF) can attenuate inflammatory response of dental pulp cells challenged with LPS. In methodology, dental pulp cells were isolated from extracted teeth. The population of DPSCs in the cultured DPCs was identified by phenotypes and multilineage differentiation. The effects of 0.4 T SMF on DPCs were observed through MTT assay and fluorescent anisotropy assay. Our results showed that the SMF exposure had no effect on surface markers or multilineage differentiation capability. However, SMF exposure increases cell viability by 15%. In addition, SMF increased cell membrane rigidity which is directly related to higher fluorescent anisotropy. In the LPS-challenged condition, DPCs treated with SMF demonstrated a higher tolerance to LPS-induced inflammatory response when compared to untreated controls. According to these results, we suggest that 0.4 T SMF attenuates LPS-induced inflammatory response to DPCs by changing cell membrane stability.

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The static magnetic field accelerates the osteogenic differentiation and mineralization of dental pulp cells

Cytotechnology ◽

10.1007/s10616-010-9271-3 ◽

2010 ◽

Vol 62 (2) ◽

pp. 143-155 ◽

Cited By ~ 35

Author(s):

Shan-hui Hsu ◽

Jui-Chih Chang

Keyword(s):

Magnetic Field ◽

Osteogenic Differentiation ◽

Static Magnetic Field ◽

Dental Pulp ◽

Dental Pulp Cells ◽

Pulp Cells

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Betamethasone suppresses the inflammatory response in LPS-stimulated dental pulp cells through inhibition of NF-κB

Archives of Oral Biology ◽

10.1016/j.archoralbio.2018.11.022 ◽

2019 ◽

Vol 98 ◽

pp. 156-163 ◽

Cited By ~ 3

Author(s):

Dan Wang ◽

Ning-Xin Zhu ◽

Man Qin ◽

Yuan-Yuan Wang

Keyword(s):

Inflammatory Response ◽

Dental Pulp ◽

Dental Pulp Cells ◽

Pulp Cells

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XPC Promotes Pluripotency of Human Dental Pulp Cells through Regulation of Oct-4/Sox2/c-Myc

Stem Cells International ◽

10.1155/2016/3454876 ◽

2016 ◽

Vol 2016 ◽

pp. 1-12 ◽

Cited By ~ 1

Author(s):

Lu Liu ◽

Zhengjun Peng ◽

Zhezhen Xu ◽

Xi Wei

Keyword(s):

Western Blot ◽

Real Time ◽

Real Time Pcr ◽

Dental Pulp ◽

Dental Pulp Cells ◽

Multilineage Differentiation ◽

Human Dental Pulp Cells ◽

Human Dental Pulp ◽

Pulp Cells

Introduction. Xeroderma pigmentosum group C (XPC), essential component of multisubunit stem cell coactivator complex (SCC), functions as the critical factor modulating pluripotency and genome integrity through interaction with Oct-4/Sox2. However, its specific role in regulating pluripotency and multilineage differentiation of human dental pulp cells (DPCs) remains unknown. Methods. To elucidate the functional role XPC played in pluripotency and multilineage differentiation of DPCs, expressions of XPC in DPCs with long-term culture were examined by real-time PCR and western blot. DPCs were transfected with lentiviral-mediated human XPC gene; then transfection rate was investigated by real-time PCR and western blot. Cell cycle, apoptosis, proliferation, senescence, multilineage differentiation, and expression of Oct-4/Sox2/c-Myc in transfected DPCs were examined. Results. XPC, Oct-4, Sox2, and c-Myc were downregulated at P7 compared with P3 in DPCs with long-term culture. XPC genes were upregulated in DPCs at P2 after transfection and maintained high expression level at P3 and P7. Cell proliferation, PI value, and telomerase activity were enhanced, whereas apoptosis was suppressed in transfected DPCs. Oct-4/Sox2/c-Myc were significantly upregulated, and multilineage differentiation in DPCs with XPC overexpression was enhanced after transfection. Conclusions. XPC plays an essential role in the modulation of pluripotency and multilineage differentiation of DPCs through regulation of Oct-4/Sox2/c-Myc.

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