scholarly journals Clinicopathological significance of loss of heterozygosity in intestinal- and solid-type gastric carcinomas: a comprehensive study using the crypt isolation technique

2006 ◽  
Vol 19 (4) ◽  
pp. 548-555 ◽  
Author(s):  
Yu-Fei Jiao ◽  
Tamotsu Sugai ◽  
Wataru Habano ◽  
Noriyuki Uesugi ◽  
Akinori Takagane ◽  
...  
Keyword(s):  
Loss Of Heterozygosity ◽  
Isolation Technique ◽  
Gastric Carcinomas ◽  
Clinicopathological Significance ◽  
Crypt Isolation ◽  
Solid Type ◽  
Comprehensive Study

scholarly journals Genetic alterations in DNA diploid, aneuploid and multiploid colorectal carcinomas identified by the crypt isolation technique

2000 ◽  
Vol 88 (4) ◽  
pp. 614-619 ◽  
Author(s):  
Tamotsu Sugai ◽  
Wataru Habano ◽  
Shin-ichi Nakamura ◽  
Hajime Sato ◽  
Noriyuki Uesugi ◽  
...  
Keyword(s):  
Genetic Alterations ◽  
Colorectal Carcinomas ◽  
Isolation Technique ◽  
Crypt Isolation

A Unique Method for Mutation Analysis of Tumor Suppressor Genes in Colorectal Carcinomas Using a Crypt Isolation Technique

2000 ◽  
Vol 124 (3) ◽  
pp. 382-386 ◽  
Author(s):  
Tamotsu Sugai ◽  
Wataru Habano ◽  
Shin-ichi Nakamura ◽  
Noriyuki Uesugi ◽  
Shunichi Sasou ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Genetic Analysis ◽  
Tumor Suppressor Genes ◽  
Single Strand Conformation Polymorphism ◽  
Single Strand ◽  
Colorectal Carcinomas ◽  
Suppressor Genes ◽  
Isolation Technique ◽  
Crypt Isolation ◽  
Conformation Polymorphism

Abstract Background.—Contamination of nontumor tissue makes genetic analysis difficult. For this reason, it is important to obtain pure tumor tissue to ensure accurate genetic analysis. Objective.—To accurately assess the incidence of mutation of tumor suppressor genes (p53: exon 5–8; APC: mutated cluster region; NF-2 gene: all exons) in 45 colorectal carcinomas. Methods.—We developed an application of the polymerase chain reaction–single-strand conformation polymorphism and DNA sequence by coupling them with crypt isolation. Results.—Mutations of p53 and APC genes were found in 24 and 22 of 45 colorectal carcinomas, respectively. No mutation of the NF-2 gene was observed in this cancer. Single-strand conformation polymorphism using a crypt isolation technique showed a clear migrating band and no false-positive data. Conclusions.—The crypt isolation technique is a useful method for accurately analyzing genetic alterations. Furthermore, our proposed method confirmed the morphological findings obtained before the genetic analysis.

Application of the crypt-isolation technique to flow-cytometric analysis of DNA content in colorectal neoplasms

1994 ◽  
Vol 106 (1) ◽  
pp. 100-107 ◽  
Author(s):  
Shin-Ichi Nakamura ◽  
Junko Goto ◽  
Masahiko Kitayama ◽  
Isamu Kino
Keyword(s):  
Colorectal Neoplasms ◽  
Dna Content ◽  
Flow Cytometric Analysis ◽  
Isolation Technique ◽  
Flow Cytometric ◽  
Crypt Isolation

Loss of Heterozygosity and Microsatellite Instability at Multiple Tumor Suppressor Genes in Gastric Carcinomas

2003 ◽  
Vol 3 (4) ◽  
pp. 214 ◽  
Author(s):  
Young Gu Cho ◽  
Chang Jae Kim ◽  
Cho Hyun Park ◽  
Young Sil Kim ◽  
Su Young Kim ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Microsatellite Instability ◽  
Loss Of Heterozygosity ◽  
Tumor Suppressor Genes ◽  
Suppressor Genes ◽  
Gastric Carcinomas ◽  
Multiple Tumor

scholarly journals Loss of Heterozygosity on the Short Arm of Chromosome 9 without p16 Gene Mutation in Gastric Carcinomas

1995 ◽  
Vol 86 (4) ◽  
pp. 333-335 ◽  
Author(s):  
Ken Sakata ◽  
Gen Tamura ◽  
Chihaya Maesawa ◽  
Yasushi Suzuki ◽  
Masanori Terashima ◽  
...  
Keyword(s):  
Gene Mutation ◽  
Loss Of Heterozygosity ◽  
Chromosome 9 ◽  
P16 Gene ◽  
Gastric Carcinomas

Use of Crypt Isolation to Determine Loss of Heterozygosity of Multiple Tumor Suppressor Genes in Colorectal Carcinoma

2000 ◽  
Vol 196 (3) ◽  
pp. 145-150 ◽  
Author(s):  
Tamotsu Sugai ◽  
Wataru Habano ◽  
Shin-ichi Nakamura ◽  
Toru Yoshida ◽  
Noriyuki Uesugi ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Colorectal Carcinoma ◽  
Loss Of Heterozygosity ◽  
Tumor Suppressor Genes ◽  
Suppressor Genes ◽  
Multiple Tumor ◽  
Crypt Isolation

A comprehensive study of (CH3)2CHOC(S)SC(O)OCH3using matrix isolation technique, X-ray analysis, spectroscopic studies and theoretical calculations

2009 ◽  
Vol 22 (9) ◽  
pp. 815-822 ◽  
Author(s):  
Yeny A. Tobón ◽  
Rosana M. Romano ◽  
Evamarie Hey-Hawkins ◽  
Roland Boese ◽  
Carlos O. Della Védova
Keyword(s):  
Theoretical Calculations ◽  
Matrix Isolation ◽  
Spectroscopic Studies ◽  
Isolation Technique ◽  
X Ray ◽  
Comprehensive Study

Identification of a new target region by loss of heterozygosity at 5p15.33 in sporadic gastric carcinomas: genotype and phenotype related

2005 ◽  
Vol 224 (2) ◽  
pp. 329-337 ◽  
Author(s):  
Yun Lu ◽  
Yingyan Yu ◽  
Zhenggang Zhu ◽  
Heng Xu ◽  
Jun Ji ◽  
...  
Keyword(s):  
Loss Of Heterozygosity ◽  
Target Region ◽  
Gastric Carcinomas
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