scholarly journals Membrane curvature sensing by the C-terminal domain of complexin

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
David Snead ◽  
Rachel T. Wragg ◽  
Jeremy S. Dittman ◽  
David Eliezer
Keyword(s):  
Membrane Curvature ◽  
Curvature Sensing ◽  
Terminal Domain

scholarly journals C-terminal domain of mammalian complexin-1 localizes to highly curved membranes

2016 ◽  
Vol 113 (47) ◽  
pp. E7590-E7599 ◽  
Author(s):  
Jihong Gong ◽  
Ying Lai ◽  
Xiaohong Li ◽  
Mengxian Wang ◽  
Jeremy Leitz ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Neurotransmitter Release ◽  
Membrane Curvature ◽  
Neuronal Cultures ◽  
Spontaneous Release ◽  
Vesicle Membrane ◽  
Curvature Sensing ◽  
Postsynaptic Currents ◽  
Terminal Domain

In presynaptic nerve terminals, complexin regulates spontaneous “mini” neurotransmitter release and activates Ca2+-triggered synchronized neurotransmitter release. We studied the role of the C-terminal domain of mammalian complexin in these processes using single-particle optical imaging and electrophysiology. The C-terminal domain is important for regulating spontaneous release in neuronal cultures and suppressing Ca2+-independent fusion in vitro, but it is not essential for evoked release in neuronal cultures and in vitro. This domain interacts with membranes in a curvature-dependent fashion similar to a previous study with worm complexin [Snead D, Wragg RT, Dittman JS, Eliezer D (2014) Membrane curvature sensing by the C-terminal domain of complexin. Nat Commun 5:4955]. The curvature-sensing value of the C-terminal domain is comparable to that of α-synuclein. Upon replacement of the C-terminal domain with membrane-localizing elements, preferential localization to the synaptic vesicle membrane, but not to the plasma membrane, results in suppression of spontaneous release in neurons. Membrane localization had no measurable effect on evoked postsynaptic currents of AMPA-type glutamate receptors, but mislocalization to the plasma membrane increases both the variability and the mean of the synchronous decay time constant of NMDA-type glutamate receptor evoked postsynaptic currents.

Osmotically-induced tension and the binding of N-BAR protein to lipid vesicles

Soft Matter ◽  
2016 ◽  
Vol 12 (8) ◽  
pp. 2465-2472 ◽  
Author(s):  
Jaime B. Hutchison ◽  
Aruni P. K. K. Karunanayake Mudiyanselage ◽  
Robert M. Weis ◽  
Anthony D. Dinsmore
Keyword(s):  
Osmotic Stress ◽  
Binding Affinity ◽  
Lipid Vesicles ◽  
Membrane Curvature ◽  
Protein Domain ◽  
Curvature Sensing

The binding affinity of a curvature-sensing protein domain (N-BAR) is measured as a function of applied osmotic stress while the membrane curvature is nearly constant.

Enrichment of membrane curvature-sensing proteins from Escherichia coli using spherical supported lipid bilayers

2021 ◽  
Author(s):  
Masayoshi Tanaka ◽  
Yu Ueno ◽  
Takahiro Miyake ◽  
Takahiro Sakuma ◽  
Mina Okochi
Keyword(s):  
Escherichia Coli ◽  
Lipid Bilayers ◽  
Membrane Curvature ◽  
Supported Lipid Bilayers ◽  
Curvature Sensing

scholarly journals PIP2 Lipids as Regulators of Membrane Curvature Sensing by Enth Domains

2019 ◽  
Vol 116 (3) ◽  
pp. 92a
Author(s):  
Alexis Belessiotis-Richards ◽  
Molly M. Stevens ◽  
Alfredo Alexander-Katz
Keyword(s):  
Membrane Curvature ◽  
Curvature Sensing

scholarly journals Amphipathic helical peptide-based fluorogenic probes for a marker-free analysis of exosomes based on membrane-curvature sensing

RSC Advances ◽  
2020 ◽  
Vol 10 (63) ◽  
pp. 38323-38327
Author(s):  
Yusuke Sato ◽  
Kazuki Kuwahara ◽  
Kenta Mogami ◽  
Kenta Takahashi ◽  
Seiichi Nishizawa
Keyword(s):  
Membrane Curvature ◽  
Helical Peptides ◽  
Curvature Sensing ◽  
Helical Peptide ◽  
Fluorogenic Probes ◽  
Marker Free

Fluorogenic probes based on membrane curvature sensing-amphipathic helical peptides have been developed for a marker-free exosome analysis.

scholarly journals An amphipathic helix enables septins to sense micrometer-scale membrane curvature

2019 ◽  
Vol 218 (4) ◽  
pp. 1128-1137 ◽  
Author(s):  
Kevin S. Cannon ◽  
Benjamin L. Woods ◽  
John M. Crutchley ◽  
Amy S. Gladfelter
Keyword(s):  
Membrane Curvature ◽  
Amphipathic Helix ◽  
Curvature Sensing ◽  
C Terminus ◽  
Number Of Binding Sites ◽  
Curved Membranes ◽  
Necessary And Sufficient ◽  
Micrometer Scale

Cell shape is well described by membrane curvature. Septins are filament-forming, GTP-binding proteins that assemble on positive, micrometer-scale curvatures. Here, we examine the molecular basis of curvature sensing by septins. We show that differences in affinity and the number of binding sites drive curvature-specific adsorption of septins. Moreover, we find septin assembly onto curved membranes is cooperative and show that geometry influences higher-order arrangement of septin filaments. Although septins must form polymers to stay associated with membranes, septin filaments do not have to span micrometers in length to sense curvature, as we find that single-septin complexes have curvature-dependent association rates. We trace this ability to an amphipathic helix (AH) located on the C-terminus of Cdc12. The AH domain is necessary and sufficient for curvature sensing both in vitro and in vivo. These data show that curvature sensing by septins operates at much smaller length scales than the micrometer curvatures being detected.

scholarly journals Membrane Curvature Sensing by Amphipathic Helices

2011 ◽  
Vol 286 (49) ◽  
pp. 42603-42614 ◽  
Author(s):  
Martin Borch Jensen ◽  
Vikram Kjøller Bhatia ◽  
Christine C. Jao ◽  
Jakob Ewald Rasmussen ◽  
Søren L. Pedersen ◽  
...  
Keyword(s):  
Membrane Curvature ◽  
Curvature Sensing ◽  
Amphipathic Helices

scholarly journals BIN1 Membrane Curvature Sensing and Generation Show Autoinhibition Regulated by Downstream Ligands and PI(4,5)P2

Biochemistry ◽  
2014 ◽  
Vol 53 (46) ◽  
pp. 7297-7309 ◽  
Author(s):  
Tingting Wu ◽  
Tobias Baumgart
Keyword(s):  
Membrane Curvature ◽  
Curvature Sensing

scholarly journals An N-terminal domain of adenovirus protein VI fragments membranes by inducing positive membrane curvature

Virology ◽  
2010 ◽  
Vol 402 (1) ◽  
pp. 11-19 ◽  
Author(s):  
Oana Maier ◽  
Debra L. Galan ◽  
Harald Wodrich ◽  
Christopher M. Wiethoff
Keyword(s):  
Membrane Curvature ◽  
Terminal Domain ◽  
Protein Vi
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