Osmotically-induced tension and the binding of N-BAR protein to lipid vesicles

Soft Matter ◽  
2016 ◽  
Vol 12 (8) ◽  
pp. 2465-2472 ◽  
Author(s):  
Jaime B. Hutchison ◽  
Aruni P. K. K. Karunanayake Mudiyanselage ◽  
Robert M. Weis ◽  
Anthony D. Dinsmore
Keyword(s):  
Osmotic Stress ◽  
Binding Affinity ◽  
Lipid Vesicles ◽  
Membrane Curvature ◽  
Protein Domain ◽  
Curvature Sensing

The binding affinity of a curvature-sensing protein domain (N-BAR) is measured as a function of applied osmotic stress while the membrane curvature is nearly constant.

scholarly journals Membrane curvature sensing by the C-terminal domain of complexin

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
David Snead ◽  
Rachel T. Wragg ◽  
Jeremy S. Dittman ◽  
David Eliezer
Keyword(s):  
Membrane Curvature ◽  
Curvature Sensing ◽  
Terminal Domain

Enrichment of membrane curvature-sensing proteins from Escherichia coli using spherical supported lipid bilayers

2021 ◽  
Author(s):  
Masayoshi Tanaka ◽  
Yu Ueno ◽  
Takahiro Miyake ◽  
Takahiro Sakuma ◽  
Mina Okochi
Keyword(s):  
Escherichia Coli ◽  
Lipid Bilayers ◽  
Membrane Curvature ◽  
Supported Lipid Bilayers ◽  
Curvature Sensing

scholarly journals PIP2 Lipids as Regulators of Membrane Curvature Sensing by Enth Domains

2019 ◽  
Vol 116 (3) ◽  
pp. 92a
Author(s):  
Alexis Belessiotis-Richards ◽  
Molly M. Stevens ◽  
Alfredo Alexander-Katz
Keyword(s):  
Membrane Curvature ◽  
Curvature Sensing

scholarly journals Amphipathic helical peptide-based fluorogenic probes for a marker-free analysis of exosomes based on membrane-curvature sensing

RSC Advances ◽  
2020 ◽  
Vol 10 (63) ◽  
pp. 38323-38327
Author(s):  
Yusuke Sato ◽  
Kazuki Kuwahara ◽  
Kenta Mogami ◽  
Kenta Takahashi ◽  
Seiichi Nishizawa
Keyword(s):  
Membrane Curvature ◽  
Helical Peptides ◽  
Curvature Sensing ◽  
Helical Peptide ◽  
Fluorogenic Probes ◽  
Marker Free

Fluorogenic probes based on membrane curvature sensing-amphipathic helical peptides have been developed for a marker-free exosome analysis.

scholarly journals An amphipathic helix enables septins to sense micrometer-scale membrane curvature

2019 ◽  
Vol 218 (4) ◽  
pp. 1128-1137 ◽  
Author(s):  
Kevin S. Cannon ◽  
Benjamin L. Woods ◽  
John M. Crutchley ◽  
Amy S. Gladfelter
Keyword(s):  
Membrane Curvature ◽  
Amphipathic Helix ◽  
Curvature Sensing ◽  
C Terminus ◽  
Number Of Binding Sites ◽  
Curved Membranes ◽  
Necessary And Sufficient ◽  
Micrometer Scale

Cell shape is well described by membrane curvature. Septins are filament-forming, GTP-binding proteins that assemble on positive, micrometer-scale curvatures. Here, we examine the molecular basis of curvature sensing by septins. We show that differences in affinity and the number of binding sites drive curvature-specific adsorption of septins. Moreover, we find septin assembly onto curved membranes is cooperative and show that geometry influences higher-order arrangement of septin filaments. Although septins must form polymers to stay associated with membranes, septin filaments do not have to span micrometers in length to sense curvature, as we find that single-septin complexes have curvature-dependent association rates. We trace this ability to an amphipathic helix (AH) located on the C-terminus of Cdc12. The AH domain is necessary and sufficient for curvature sensing both in vitro and in vivo. These data show that curvature sensing by septins operates at much smaller length scales than the micrometer curvatures being detected.

scholarly journals Membrane Curvature Sensing by Amphipathic Helices

2011 ◽  
Vol 286 (49) ◽  
pp. 42603-42614 ◽  
Author(s):  
Martin Borch Jensen ◽  
Vikram Kjøller Bhatia ◽  
Christine C. Jao ◽  
Jakob Ewald Rasmussen ◽  
Søren L. Pedersen ◽  
...  
Keyword(s):  
Membrane Curvature ◽  
Curvature Sensing ◽  
Amphipathic Helices

scholarly journals BIN1 Membrane Curvature Sensing and Generation Show Autoinhibition Regulated by Downstream Ligands and PI(4,5)P2

Biochemistry ◽  
2014 ◽  
Vol 53 (46) ◽  
pp. 7297-7309 ◽  
Author(s):  
Tingting Wu ◽  
Tobias Baumgart
Keyword(s):  
Membrane Curvature ◽  
Curvature Sensing

scholarly journals Fast Simulation of Lipid Vesicle Deformation Using Spherical Harmonic Approximation

2016 ◽  
Vol 21 (1) ◽  
pp. 40-64
Author(s):  
Michael Mikucki ◽  
Yongcheng Zhou
Keyword(s):  
Harmonic Functions ◽  
Degrees Of Freedom ◽  
Finite Difference Methods ◽  
Harmonic Approximation ◽  
Lipid Vesicles ◽  
Membrane Curvature ◽  
Lipid Vesicle ◽  
Nonlinear Conjugate Gradient Method ◽  
Surface Harmonic ◽  
Curvature Energy

AbstractLipid vesicles appear ubiquitously in biological systems. Understanding how the mechanical and intermolecular interactions deform vesicle membranes is a fundamental question in biophysics. In this article we develop a fast algorithm to compute the surface configurations of lipid vesicles by introducing surface harmonic functions to approximate themembrane surface. This parameterization allows an analytical computation of the membrane curvature energy and its gradient for the efficient minimization of the curvature energy using a nonlinear conjugate gradient method. Our approach drastically reduces the degrees of freedom for approximating the membrane surfaces compared to the previously developed finite element and finite difference methods. Vesicle deformations with a reduced volume larger than 0.65 can be well approximated by using as small as 49 surface harmonic functions. The method thus has a great potential to reduce the computational expense of tracking multiple vesicles which deform for their interaction with external fields.

scholarly journals Irreversible Thermodynamics of Lipid Vesicles under Osmotic Stress

2016 ◽  
Vol 110 (3) ◽  
pp. 371a
Author(s):  
Morgan Chabanon ◽  
James Ho ◽  
Atul N. Parikh ◽  
Padmini Rangamani
Keyword(s):  
Osmotic Stress ◽  
Lipid Vesicles ◽  
Irreversible Thermodynamics
Sign in / Sign up

Export Citation Format

Share Document