Real-time single-molecule coimmunoprecipitation of weak protein-protein interactions

2013 ◽  
Vol 8 (10) ◽  
pp. 2045-2060 ◽  
Author(s):  
Hong-Won Lee ◽  
Ji Young Ryu ◽  
Janghyun Yoo ◽  
Byungsan Choi ◽  
Kipom Kim ◽  
...  
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Protein Interactions ◽  
Protein Protein Interactions

scholarly journals Single-molecule nanopore sensing of actin dynamics and drug binding

2020 ◽  
Vol 11 (4) ◽  
pp. 970-979 ◽  
Author(s):  
Xiaoyi Wang ◽  
Mark D. Wilkinson ◽  
Xiaoyan Lin ◽  
Ren Ren ◽  
Keith R. Willison ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Real Time ◽  
Single Molecule ◽  
Protein Interactions ◽  
Drug Binding ◽  
Actin Dynamics ◽  
Protein Protein Interactions

Nanopipettes were used for real-time investigation into actin dynamics and drug binding at single-molecule resolution, showing promise for a better understanding of the mechanism of protein–protein interactions and drug discovery.

scholarly journals EndoBind detects endogenous protein-protein interactions in real time

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Anke Bill ◽  
Sheryll Espinola ◽  
Daniel Guthy ◽  
Jacob R. Haling ◽  
Mylene Lanter ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Protein Interactions ◽  
Continuous Monitoring ◽  
Protein Protein Interactions ◽  
Dimer Formation ◽  
Endogenous Protein ◽  
Extended Duration ◽  
Substrate Addition

AbstractWe present two high-throughput compatible methods to detect the interaction of ectopically expressed (RT-Bind) or endogenously tagged (EndoBind) proteins of interest. Both approaches provide temporal evaluation of dimer formation over an extended duration. Using examples of the Nrf2-KEAP1 and the CRAF-KRAS-G12V interaction, we demonstrate that our method allows for the detection of signal for more than 2 days after substrate addition, allowing for continuous monitoring of endogenous protein-protein interactions in real time.

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