scholarly journals A new tool for technical standardization of the Ki67 immunohistochemical assay

2021 ◽  
Author(s):  
Thazin Nwe Aung ◽  
Balazs Acs ◽  
Jonathan Warrell ◽  
Yalai Bai ◽  
Patricia Gaule ◽  
...  
Keyword(s):  
Immunohistochemical Assay ◽  
Technical Standardization

scholarly journals Changes of Caspase-1 after the Application of Orthodontic Forces in the Periodontal Tissues of Rats

2009 ◽  
Vol 79 (6) ◽  
pp. 1126-1132 ◽  
Author(s):  
Xiulin Yan ◽  
Jiang Chen ◽  
Yuquan Hao ◽  
Yan Wang ◽  
Li Zhu
Keyword(s):  
Western Blotting ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Traction Force ◽  
Histomorphometric Analysis ◽  
Periodontal Tissues ◽  
Immunohistochemical Assay ◽  
Caspase 1 ◽  
Orthodontic Forces ◽  
Hematoxylin Eosin

Abstract Objective: To investigate the changes of caspase-1 in orthodontic tooth movement and to determine whether the changes are phase-specific. Materials and Methods: Eighty Wistar rats were included in this study. Sentalloy closed-coil springs were placed to induce a mesial traction force on the lower right first molar. The animals were killed after 1, 3, 7, and 14 days (n = 20 at each time point). The mandibles of 10 rats were sampled for histomorphometric analysis and immunohistochemical assay, and the periodontal tissues of 10 other rats were sampled for detecting caspase-1 mRNA and protein by real-time RT-PCR and by Western blotting, respectively. Results: The inflammatory reaction was evident in paraffin sections with hematoxylin-eosin staining. The immunohistochemical assay showed that orthodontic forces significantly increased the number of caspase-1-positive cells in the periodontal ligament (PDL). Mechanical force triggered an increase of caspase-1 mRNA in periodontal tissues. The expression of caspase-1 mRNA increased from day 1, reached the peak on day 3, and then decreased. The results of Western blotting indicated that the levels of both procaspase-1 and P20 subunit significantly increased after the application of orthodontic forces, compared with those in controls (P < .05). Conclusion: Caspase-1 level increases during orthodontic tooth movement and changes with different phases, which might play a significant role in orthodontic tooth movement.

scholarly journals Leishmania spp. parasite isolation through inoculation of patient biopsy macerates in interferon gamma knockout mice

2010 ◽  
Vol 52 (2) ◽  
pp. 83-88 ◽  
Author(s):  
Milton Adriano Pelli de Oliveira ◽  
Alause da Silva Pires ◽  
Rosidete Pereira de Bastos ◽  
Glória Maria Collet de Araujo Lima ◽  
Sebastião Alves Pinto ◽  
...  
Keyword(s):  
Species Identification ◽  
Interferon Gamma ◽  
Knockout Mice ◽  
New World ◽  
Clinical Signs ◽  
Leishmania Species ◽  
Mouse Strains ◽  
Preliminary Results ◽  
Immunohistochemical Assay ◽  
Leishmania Spp

Isolation of Leishmania parasite and species identification are important for confirmation and to help define the epidemiology of the leishmaniasis. Mice are often used to isolate pathogens, but the most common mouse strains are resistant to infection with parasites from the Leishmania (Viannia) subgenus. In this study we tested the inoculation of interferon gamma knockout (IFNγ KO) mice with biopsy macerates from Leishmania-infected patients to increase the possibility of isolating parasites. Biopsies from twenty five patients with clinical signs of leishmaniasis were taken and tested for the presence of parasites. Immunohistochemical assay (IHC) and conventional histopathology detected the parasite in 88% and 83% of the patients, respectively. Leishmania sp. were isolated in biopsy macerates from 52% of the patients by culture in Grace's insect medium, but 13% of isolates were lost due to contamination. Inoculation of macerates in IFNγ KO mice provides isolation of parasites in 31.8% of the biopsies. Most isolates belong to L. (Viannia) subgenus, as confirmed by PCR, except one that belongs to L. (Leishmania) subgenus. Our preliminary results support the use of IFNγ KO mice to improve the possibility to isolate New World Leishmania species.

Standardization and Intellectual Property Rights

2000 ◽  
pp. 80-101 ◽  
Author(s):  
Eric J. Iversen
Keyword(s):  
Intellectual Property ◽  
Property Rights ◽  
Information And Communication Technologies ◽  
Intellectual Property Rights ◽  
Innovation Process ◽  
Technology Infrastructure ◽  
Ict Industry ◽  
The Individual ◽  
Technical Standardization ◽  
Standard Development

In today’s environment of rapidly evolving information and communication technologies (ICTs), technical standardization is said to be confronted by a “minefield” of intellectual property rights (IPRs). Patents and other industrial IPRs that might belong to individual developers of technology have the potential to undermine the collective pursuit of technical standardization that might serve the common interests of the sector or industry. This tension between the individual and the collective, between the development of technology and its diffusion, is however by no means new; it is an inherent feature of standard development as an institution of innovation. The fact that this tension has only recently been converted into conflict raises a host of interesting questions about standardization in the evolving environment of the ‘digital age’. In this chapter, we will address some of these. We are especially interested in the fundamental question concerning the roles of standard development organizations and IPRs in the “technology infrastructure” (Tassey, 1995) and how these roles are “co-evolving” (Nelson, 1995) with the rapidly developing ICT industry. The contention is that this process of coevolution is bringing what are initially complementary functions in the innovation process into increased confrontation. In this chapter such questions will be explored in terms of innovation-theory in which the role this ‘technology infrastructure’ plays is explicitly recognized. The discussion of this relationship moreover will be largely presented in terms of a case study, featuring the controversy that arose during the standardization of the now popular GSM system, produced by the European Telecommunication Standards Institute (ETSI).

VoIP vs GSM Technology

2012 ◽  
pp. 280-298 ◽  
Author(s):  
Ikponmwosa Oghogho ◽  
Dickinson C. Odikayor ◽  
Abayomi-Alli Adebayo ◽  
Samuel T. Wara
Keyword(s):  
Communication Systems ◽  
Service Providers ◽  
Disruptive Technology ◽  
Counter Measures ◽  
Security Issues ◽  
Evolution Of Communication ◽  
Service Introduction ◽  
Technical Standardization ◽  
Intrusion Detection And Prevention

This chapter presents VoIP as a disruptive technology to GSM technology as well as the issues, controversies, and problems surrounding its deployment. It gives a general introduction of the evolution of communication systems from the POTS, to GSM, and now VoIP. Several issues that surround the deployment of VoIP such as provision of PSTN equivalent services by VoIP service providers, regulation of the service, introduction of latency and other counter measures by some operators, threat posed to PSTN providers due to emergence of VoIP, the need for technical standardization of VoIP, security issues, different cost structure, and quality of service provided were also discussed in details. Solutions and recommendations were suggested to overcome the challenges outlined. VoIP is presented as the way of the future for communication. When this finally happens depends on how fast the challenges outlined in this chapter are addressed. Future and emerging research trends in the deployment of VoIP such as locating users in a secure and reliable way, monitoring VoIP networks, as well as intrusion detection and prevention on SIP were also considered, after which, conclusion was made. This chapter is both informative and interesting.

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