scholarly journals Regulating vesicle bilayer permeability and selectivity via stimuli-triggered polymersome-to-PICsome transition

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Xiaorui Wang ◽  
Chenzhi Yao ◽  
Guoying Zhang ◽  
Shiyong Liu
Keyword(s):  
Bilayer Permeability

Biophysics (and sociology) of ceramides.

2005 ◽  
Vol 72 ◽  
pp. 177-188 ◽  
Author(s):  
Félix M. Goñi ◽  
F-Xabier Contreras ◽  
L-Ruth Montes ◽  
Jesús Sot ◽  
Alicia Alonso
Keyword(s):  
Cell Biology ◽  
Positive Curvature ◽  
Acyl Chain ◽  
Cell Signalling ◽  
Hexagonal Structure ◽  
Lipid Monolayer ◽  
Flip Flop ◽  
Long Chain ◽  
Bilayer Permeability

In the past decade, the long-neglected ceramides (N-acylsphingosines) have become one of the most attractive lipid molecules in molecular cell biology, because of their involvement in essential structures (stratum corneum) and processes (cell signalling). Most natural ceramides have a long (16-24 C atoms) N-acyl chain, but short N-acyl chain ceramides (two to six C atoms) also exist in Nature, apart from being extensively used in experimentation, because they can be dispersed easily in water. Long-chain ceramides are among the most hydrophobic molecules in Nature, they are totally insoluble in water and they hardly mix with phospholipids in membranes, giving rise to ceramide-enriched domains. In situ enzymic generation, or external addition, of long-chain ceramides in membranes has at least three important effects: (i) the lipid monolayer tendency to adopt a negative curvature, e.g. through a transition to an inverted hexagonal structure, is increased, (ii) bilayer permeability to aqueous solutes is notoriously enhanced, and (iii) transbilayer (flip-flop) lipid motion is promoted. Short-chain ceramides mix much better with phospholipids, promote a positive curvature in lipid monolayers, and their capacities to increase bilayer permeability or transbilayer motion are very low or non-existent.

scholarly journals The effect of lipopolysaccharide on lipid bilayer permeability of β-lactam antibiotics

FEBS Letters ◽  
1984 ◽  
Vol 170 (2) ◽  
pp. 268-272 ◽  
Author(s):  
Ryoichi Hiruma ◽  
Akihito Yamaguchi ◽  
Tetsuo Sawai
Keyword(s):  
Lipid Bilayer ◽  
Bilayer Permeability

Enhancement of apparent substrate selectivity of proteinase K encapsulated in liposomes through a cholate-induced alteration of the bilayer permeability

2003 ◽  
Vol 85 (2) ◽  
pp. 222-233 ◽  
Author(s):  
Makoto Yoshimoto ◽  
Shaoqing Wang ◽  
Kimitoshi Fukunaga ◽  
Mike Treyer ◽  
Peter Walde ◽  
...  
Keyword(s):  
Proteinase K ◽  
Substrate Selectivity ◽  
Bilayer Permeability

The influence of lipid composition on glycophorin-induced bilayer permeability

1984 ◽  
Vol 771 (2) ◽  
pp. 119-126 ◽  
Author(s):  
P. Van Hoogevest ◽  
A.P.M. Du Maine ◽  
B. De Kruijff ◽  
J. De Gier
Keyword(s):  
Lipid Composition ◽  
Bilayer Permeability

Photolipid Bilayer Permeability is Controlled by Transient Pore Formation

Langmuir ◽  
2020 ◽  
Vol 36 (45) ◽  
pp. 13509-13515
Author(s):  
Stefanie D. Pritzl ◽  
Patrick Urban ◽  
Alexander Prasselsperger ◽  
David B. Konrad ◽  
James A. Frank ◽  
...  
Keyword(s):  
Pore Formation ◽  
Bilayer Permeability

scholarly journals Vesicle-micelle structural transition of phosphatidylcholine bilayers and Triton X-100

1994 ◽  
Vol 303 (3) ◽  
pp. 907-914 ◽  
Author(s):  
A De la Maza ◽  
J L Parra
Keyword(s):  
Light Scattering ◽  
Size Distribution ◽  
Surfactant Concentration ◽  
Structural Transition ◽  
Initial Step ◽  
Ionic Surfactant ◽  
Unilamellar Vesicles ◽  
Multilayered Structures ◽  
Triton X ◽  
Bilayer Permeability

The structural transition stages induced by the interaction of the non-ionic surfactant Triton X-100 on phosphatidylcholine unilamellar vesicles were studied by means of static and dynamic light-scattering, transmission-electron-microscopy (t.e.m.) and permeability changes. A linear correlation was observed between the effective surfactant/lipid molar ratios (Re) (‘three-stage’ model proposed for the vesicle solubilization) and the surfactant concentration throughout the process. However, this correlation was not noted for the partition coefficients of the surfactant between the bilayer and the aqueous medium (K). Thus a sharp initial K increase was observed until a maximum value was achieved for permeability alterations of 50% (initial step of bilayer saturation). Further surfactant additions resulted in a fall in the K values until 100% of bilayer permeability. Additional amounts of surfactant led to an increase in K until bilayer solubilization. Hence, a preferential incorporation of surfactant molecules into liposomes governs the initial interaction steps, leading to the initial stage of bilayer saturation with a free surfactant concentration that was lower than its critical micelle concentration (c.m.c.). Additional amounts of surfactant increased the free surfactant until the c.m.c. was reached, after which solubilization started to occur. Thus the initial step of bilayer saturation was achieved for a smaller surfactant concentration than that for the Resat, although this concentration was the minimum needed for solubilization to start. Large unilamellar vesicles began to form as the surfactant exceeded 15 mol% (50% bilayer permeability), the maximum vesicle growth being attained for 22 mol% (400 nm). Thereafter, static light-scattering started to decrease gradually, this fall being more pronounced after 40 mol%. The t.e.m. picture for 40 mol% (Resat.) showed unilamellar vesicles, although with traces of smaller structures. From 50 mol% the size distribution curves began to show a bimodal distribution. The t.e.m. pictures for 50-64 mol% revealed tubular structures, together with open bilayer fragments. Thereafter, increasing amounts of surfactant (65-69 mol%) led to planar multilayered structures which gradually tended to form concentric and helicoidal conformations. The scattered intensity decreased to a low constant value at more than 71-72 mol%. However, the surfactant concentration for the Re(sol) (72.6 mol %) still presented traces of aggregated structures, albeit with mono-modal size-distribution curves (particle size of 50 nm). This vesicle size corresponded to the liposome solubilization via mixed-micelle formation.

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