Mucoadhesive functionality of cell wall structures from fruits and grains: Electrostatic and polymer network interactions mediated by soluble dietary polysaccharides

Callose, B-1,3-glucan, a component of cell walls, is associated with phloem sieve plates, plasmodesmata, and other cell wall structures that are formed in response to wounding or infection. Callose reacts with aniline blue to form a fluorescent complex that can be recognized in the light microscope with ultraviolet illumination. We have identified callose in cell wall protuberances that are formed spontaneously in suspension-cultured cells of S. tortuosus and in the tips of root hairs formed in sterile callus cultures of S. tortuosus. Callose deposits in root hairs are restricted to root hair tips which appear to be damaged or deformed, while normal root hair tips lack callose deposits. The callose deposits found in suspension culture cells are restricted to regions where unusual outgrowths or protuberances are formed on the cell surfaces, specifically regions that are the sites of new cell wall formation.Callose formation has been shown to be regulated by intracellular calcium levels.

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Faculty Opinions recommendation of Glycome and Proteome Components of Golgi Membranes Are Common between Two Angiosperms with Distinct Cell-Wall Structures.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.735406304.793563599 ◽

2019 ◽

Author(s):

Federica Brandizzi

Keyword(s):

Cell Wall ◽

Golgi Membranes ◽

Wall Structures ◽

Distinct Cell

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AtSYP71 Is Important for Plant Cell Wall Biosynthesis and Stress Adaptation.

10.21203/rs.3.rs-1189370/v1 ◽

2021 ◽

Author(s):

Xiaoyue Kou ◽

Hailong Zhang ◽

Xiaonan Zhao ◽

Mingjing Wang ◽

Guochen Qin ◽

...

Keyword(s):

Cell Wall ◽

Early Development ◽

Plant Development ◽

Plant Cell Wall ◽

Cell Plate ◽

Development Stage ◽

Cell Wall Biosynthesis ◽

Knockout Mutant ◽

Wall Structures ◽

Confocal Images

Abstract Background: SYP71, the plant-specific Qc-SNARE protein, is reported to regulate vesicle trafficking. SYP71 is localized on the ER, endosome, plasma membrane and cell plate, suggesting its multiple functions. Lotus SYP71 is essential for symbiotic nitrogen fixation in nodules. AtSYP71, GmSYP71 and OsSYP71 are implicated in plant resistance to pathogenesis. To date, SYP71 regulatory role on plant development remain unclear.Results: AtSYP71-knockout mutant atsyp71-4 was lethal at early development stage. Early development of AtSYP71-knockdown mutant atsyp71-2 was delayed, and stress response was also affected. Confocal images revealed that protein secretion was blocked in atsyp71-2. Transcriptomic analysis indicated that metabolism, response to environmental stimuli pathways and apoplast components were influenced in atsyp71-2. Moreover, the contents of lignin, cellulose and flavonoids as well as cell wall structures were also altered.Conclusion: Our findings suggested that AtSYP71 is essential for plant development. AtSYP71 probably regulates plant development, metabolism and environmental adaptation by affecting cell wall homeostasis via mediating secretion of materials and regulators required for cell wall biosynthesis and dynamics.

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Downregulation of p‐COUMAROYL ESTER3‐HYDROXYLASEin rice leads to altered cell wall structures and improves biomass saccharification

The Plant Journal ◽

10.1111/tpj.13988 ◽

2018 ◽

Vol 95 (5) ◽

pp. 796-811 ◽

Cited By ~ 25

Author(s):

Yuri Takeda ◽

Yuki Tobimatsu ◽

Steven D. Karlen ◽

Taichi Koshiba ◽

Shiro Suzuki ◽

...

Keyword(s):

Cell Wall ◽

Biomass Saccharification ◽

Wall Structures ◽

Altered Cell

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Differential pulp cell wall structures lead to diverse fruit textures in apple (Malus domestica)

PROTOPLASMA ◽

10.1007/s00709-021-01727-w ◽

2022 ◽

Author(s):

Ling Yang ◽

Peihua Cong ◽

Jiali He ◽

Haidong Bu ◽

Sijun Qin ◽

...

Keyword(s):

Cell Wall ◽

Malus Domestica ◽

Pulp Cell ◽

Wall Structures

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The Analysis of Bamboo Cell Wall Structures for Cellulose Nanofiber Materials

Sen i Gakkaishi ◽

10.2115/fiber.73.p-352 ◽

2017 ◽

Vol 73 (9) ◽

pp. P-352-P-354

Author(s):

YOKO OKAHISA

Keyword(s):

Cell Wall ◽

Cellulose Nanofiber ◽

Wall Structures

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Methods to quantify primary plant cell wall mechanics

Journal of Experimental Botany ◽

10.1093/jxb/erz281 ◽

2019 ◽

Vol 70 (14) ◽

pp. 3615-3648 ◽

Cited By ~ 15

Author(s):

Amir J Bidhendi ◽

Anja Geitmann

Keyword(s):

Cell Wall ◽

Plant Cell ◽

Plant Cell Wall ◽

Polymer Network ◽

Plant Cells ◽

Tensile Tests ◽

Mechanical Modeling ◽

Experimental Conditions ◽

Testing Techniques ◽

Cell Wall Mechanics

Abstract The primary plant cell wall is a dynamically regulated composite material of multiple biopolymers that forms a scaffold enclosing the plant cells. The mechanochemical make-up of this polymer network regulates growth, morphogenesis, and stability at the cell and tissue scales. To understand the dynamics of cell wall mechanics, and how it correlates with cellular activities, several experimental frameworks have been deployed in recent years to quantify the mechanical properties of plant cells and tissues. Here we critically review the application of biomechanical tool sets pertinent to plant cell mechanics and outline some of their findings, relevance, and limitations. We also discuss methods that are less explored but hold great potential for the field, including multiscale in silico mechanical modeling that will enable a unified understanding of the mechanical behavior across the scales. Our overview reveals significant differences between the results of different mechanical testing techniques on plant material. Specifically, indentation techniques seem to consistently report lower values compared with tensile tests. Such differences may in part be due to inherent differences among the technical approaches and consequently the wall properties that they measure, and partly due to differences between experimental conditions.

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