scholarly journals Automated analysis of lipid drug-response markers by combined fast and high-resolution whole cell MALDI mass spectrometry biotyping

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
David Weigt ◽  
Denis A. Sammour ◽  
Timon Ulrich ◽  
Bogdan Munteanu ◽  
Carsten Hopf
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Drug Response ◽  
Automated Analysis ◽  
Maldi Mass Spectrometry ◽  
Whole Cell

scholarly journals Mass Spectrometry Imaging Using Dynamically Harmonized FT-ICR at Million Resolving Power: Rationalizing and Optimizing Sample Preparation and Instrumental Parameters

2020 ◽  
Author(s):  
Mathieu Tiquet ◽  
Raphaël La Rocca ◽  
Daan van Kruining ◽  
Pilar Martinez-Martinez ◽  
Gauthier Eppe ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Mass Spectrometry Imaging ◽  
Mass Range ◽  
Maldi Mass Spectrometry ◽  
Mass Accuracy ◽  
Ion Current ◽  
Resolving Power ◽  
Lipid Mass ◽  
The Stability

<p><i>MALDI mass spectrometry imaging (MSI) is a powerful analytical method giving access to the 2D localizations of compounds in a thin section of a sample. To properly discern isobaric compounds in complex biological samples, dynamically harmonized ICR cell (ParaCell©) has been introduce to achieve extreme spectral resolution. However, high resolution MS images realized on a 9.4T FTICR High resolution instrument with recommended parameters suffered from an abnormal shifting of m/z ratios pixel to pixel. Resulting datasets show poor mass accuracy measurements and resolutions under estimations. By following the behavior of the Total Ion Current in function of the number of laser shots, the abnormal mass shifting phenomenon has been linked to the stability of the Total Ion Current (TIC) during images acquisitions. An optimization of laser parameters is proposed in order to limit the observed mass shift to retain machine specifications during MSI analyses. It is also shown that the method has been successfully employed to realize quality MS images with resolution above 1,000,000 in the lipid mass range across the whole image.</i></p>

Rapid and robust analytical protocol for E. coli STEC bacteria subspecies differentiation using whole cell MALDI mass spectrometry

Talanta ◽  
2018 ◽  
Vol 182 ◽  
pp. 164-170 ◽  
Author(s):  
Kevin Mclean ◽  
Javier Palarea-Albaladejo ◽  
Carol G. Currie ◽  
Lisa H.J. Imrie ◽  
Erin D.T. Manson ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Maldi Mass Spectrometry ◽  
Whole Cell ◽  
E Coli ◽  
Subspecies Differentiation ◽  
Analytical Protocol

scholarly journals Analysis of Detergent-Insoluble and Whole Cell Lysate Fractions of Resting Neutrophils Using High-Resolution Mass Spectrometry

2010 ◽  
Vol 9 (7) ◽  
pp. 3764-3764
Author(s):  
Gisele G. Tomazella ◽  
Idalete daSilva ◽  
Carolina H. Thomé ◽  
Lewis J. Greene ◽  
Christian J. Koehler ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
Whole Cell ◽  
Whole Cell Lysate ◽  
Cell Lysate ◽  
High Resolution Mass ◽  
Resolution Mass

scholarly journals Mass Spectrometry Imaging Using Dynamically Harmonized FT-ICR at Million Resolving Power: Rationalizing and Optimizing Sample Preparation and Instrumental Parameters

2020 ◽  
Author(s):  
Mathieu Tiquet ◽  
Raphaël La Rocca ◽  
Daan van Kruining ◽  
Pilar Martinez-Martinez ◽  
Gauthier Eppe ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Mass Spectrometry Imaging ◽  
Mass Range ◽  
Maldi Mass Spectrometry ◽  
Mass Accuracy ◽  
Ion Current ◽  
Resolving Power ◽  
Lipid Mass ◽  
The Stability

<p><i>MALDI mass spectrometry imaging (MSI) is a powerful analytical method giving access to the 2D localizations of compounds in a thin section of a sample. To properly discern isobaric compounds in complex biological samples, dynamically harmonized ICR cell (ParaCell©) has been introduce to achieve extreme spectral resolution. However, high resolution MS images realized on a 9.4T FTICR High resolution instrument with recommended parameters suffered from an abnormal shifting of m/z ratios pixel to pixel. Resulting datasets show poor mass accuracy measurements and resolutions under estimations. By following the behavior of the Total Ion Current in function of the number of laser shots, the abnormal mass shifting phenomenon has been linked to the stability of the Total Ion Current (TIC) during images acquisitions. An optimization of laser parameters is proposed in order to limit the observed mass shift to retain machine specifications during MSI analyses. It is also shown that the method has been successfully employed to realize quality MS images with resolution above 1,000,000 in the lipid mass range across the whole image.</i></p>

Application of high-resolution ESI and MALDI mass spectrometry to metabolite profiling of small interfering RNA duplex

2012 ◽  
Vol 47 (8) ◽  
pp. 1015-1022 ◽  
Author(s):  
Hisao Shimizu ◽  
Fumihiro Jinno ◽  
Akio Morohashi ◽  
Yuzo Yamazaki ◽  
Masaki Yamada ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Metabolite Profiling ◽  
Small Interfering Rna ◽  
Maldi Mass Spectrometry ◽  
Interfering Rna

scholarly journals Detection and Identification of Bioanalytes with High Resolution LSPR Spectroscopy and MALDI Mass Spectrometry

2009 ◽  
Vol 113 (15) ◽  
pp. 5891-5894 ◽  
Author(s):  
Jeffrey N. Anker ◽  
W. Paige Hall ◽  
Mary P. Lambert ◽  
Pauline T. Velasco ◽  
Milan Mrksich ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Maldi Mass Spectrometry ◽  
Detection And Identification
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