scholarly journals Screening inducers of neuronal BDNF gene transcription using primary cortical cell cultures from BDNF-luciferase transgenic mice

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mamoru Fukuchi ◽  
Yui Okuno ◽  
Hironori Nakayama ◽  
Aoi Nakano ◽  
Hisashi Mori ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Gene Transcription ◽  
Cell Cultures ◽  
Cortical Cell ◽  
Bdnf Gene ◽  
Cortical Cell Cultures ◽  
Primary Cortical Cell

scholarly journals Quercetin Attenuates Oxygen–Glucose Deprivation- and Excitotoxin-Induced Neurotoxicity in Primary Cortical Cell Cultures

2003 ◽  
Vol 26 (4) ◽  
pp. 544-546 ◽  
Author(s):  
Hyun Joo Ha ◽  
Yong Soo Kwon ◽  
Shin Mi Park ◽  
Taekyun Shin ◽  
Jun Hong Park ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Cortical Cell ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Cortical Cell Cultures ◽  
Primary Cortical Cell

scholarly journals NMDA Receptor-Mediated Neuroprotective Effect of theScutellaria baicalensisGeorgi Extract on the Excitotoxic Neuronal Cell Death in Primary Rat Cortical Cell Cultures

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Jinsong Yang ◽  
Xiaohong Wu ◽  
Haogang Yu ◽  
Xinbiao Liao ◽  
Lisong Teng
Keyword(s):  
Cell Death ◽  
Nmda Receptor ◽  
Cell Cultures ◽  
Neuroprotective Effect ◽  
Neuronal Cell Death ◽  
Cortical Cell ◽  
Neuronal Cell ◽  
Ethanol Extract ◽  
Phytochemical Analysis ◽  
Cortical Cell Cultures

The objective of the current research work was to evaluate the neuroprotective effect of the ethanol extract ofScutellaria baicalensis(S.B.) on the excitotoxic neuronal cell death in primary rat cortical cell cultures. The inhibitory effects of the extract were qualitatively and quantitatively estimated by phase-contrast microscopy and lactate dehydrogenase (LDH) assays. The extract exhibited a potent and dose-dependent inhibition of the glutamate-induced excitotoxicity in the culture media. Further, using radioligand binding assays, it was observed that the inhibitory effect of the extract was more potent and selective for the N-methyl-D-aspartate (NMDA) receptor-mediated toxicity. The S.B. ethanol extract competed with [3H] MDL 105,519 for the specific binding to the NMDA receptor glycine site with 50% inhibition occurring at 35.1 μg/mL. Further, NMDA receptor inactivation by the S.B. ethanol extract was concluded from the decreasing binding capability of [3H]MK-801 in the presence of the extract. Thus, S.B. extract exhibited neuroprotection against excitotoxic cell death, and this neuroprotection was mediated through the inhibition of NMDA receptor function by interacting with the glycine binding site of the NMDA receptor. Phytochemical analysis of the bioactive extract revealed the presence of six phytochemical constituents including baicalein, baicalin, wogonin, wogonoside, scutellarin, and Oroxylin A.

scholarly journals Ca2+-Mediated Activation of c-Jun N-Terminal Kinase and Nuclear Factor κB by NMDA in Cortical Cell Cultures

2002 ◽  
Vol 71 (4) ◽  
pp. 1390-1395 ◽  
Author(s):  
Hyuk Wan Ko ◽  
Kye-Yoon Park ◽  
Hansin Kim ◽  
Pyung-Lim Han ◽  
Youn Uck Kim ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Cortical Cell ◽  
Nuclear Factor Κb ◽  
Nuclear Factor ◽  
Cortical Cell Cultures

Oxygen or glucose deprivation-induced neuronal injury in cortical cell cultures is reduced by tetanus toxin

Neuron ◽  
1992 ◽  
Vol 8 (5) ◽  
pp. 967-973 ◽  
Author(s):  
H. Monyer ◽  
R.G. Giffard ◽  
D.M. Hartley ◽  
L.L. Dugan ◽  
M.P. Goldberg ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Tetanus Toxin ◽  
Cortical Cell ◽  
Neuronal Injury ◽  
Glucose Deprivation ◽  
Cortical Cell Cultures
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