scholarly journals Discovery of Afifi, the shallowest and southernmost brine pool reported in the Red Sea

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Carlos M. Duarte ◽  
Anders Røstad ◽  
Grégoire Michoud ◽  
Alan Barozzi ◽  
Giuseppe Merlino ◽  
...  
Keyword(s):  
Red Sea ◽  
Brine Pool

A deep sea community at the Kebrit brine pool in the Red Sea

2015 ◽  
Vol 46 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Hege Vestheim ◽  
Stein Kaartvedt
Keyword(s):  
Red Sea ◽  
Deep Sea ◽  
Brine Pool

scholarly journals Insights into Red Sea Brine Pool Specialized Metabolism Gene Clusters Encoding Potential Metabolites for Biotechnological Applications and Extremophile Survival

Marine Drugs ◽  
2019 ◽  
Vol 17 (5) ◽  
pp. 273 ◽  
Author(s):  
Laila Ziko ◽  
Mustafa Adel ◽  
Mohamed N. Malash ◽  
Rania Siam
Keyword(s):  
Fatty Acids ◽  
Red Sea ◽  
Gene Clusters ◽  
Specialized Metabolism ◽  
Anticancer Effects ◽  
Novel Drugs ◽  
Atlantis Ii Deep ◽  
Brine Pools ◽  
Metabolism Gene ◽  
Brine Pool

The recent rise in antibiotic and chemotherapeutic resistance necessitates the search for novel drugs. Potential therapeutics can be produced by specialized metabolism gene clusters (SMGCs). We mined for SMGCs in metagenomic samples from Atlantis II Deep, Discovery Deep and Kebrit Deep Red Sea brine pools. Shotgun sequence assembly and secondary metabolite analysis shell (antiSMASH) screening unraveled 2751 Red Sea brine SMGCs, pertaining to 28 classes. Predicted categorization of the SMGC products included those (1) commonly abundant in microbes (saccharides, fatty acids, aryl polyenes, acyl-homoserine lactones), (2) with antibacterial and/or anticancer effects (terpenes, ribosomal peptides, non-ribosomal peptides, polyketides, phosphonates) and (3) with miscellaneous roles conferring adaptation to the environment/special structure/unknown function (polyunsaturated fatty acids, ectoine, ladderane, others). Saccharide (80.49%) and putative (7.46%) SMGCs were the most abundant. Selected Red Sea brine pool sites had distinct SMGC profiles, e.g., for bacteriocins and ectoine. Top promising candidates, SMs with pharmaceutical applications, were addressed. Prolific SM-producing phyla (Proteobacteria, Actinobacteria, Cyanobacteria), were ubiquitously detected. Sites harboring the largest numbers of bacterial and archaeal phyla, had the most SMGCs. Our results suggest that the Red Sea brine niche constitutes a rich biological mine, with the predicted SMs aiding extremophile survival and adaptation.

scholarly journals Induction of apoptosis in cancer cell lines by the Red Sea brine pool bacterial extracts

2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Sunil Sagar ◽  
Luke Esau ◽  
Karie Holtermann ◽  
Tyas Hikmawan ◽  
Guishan Zhang ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Red Sea ◽  
Cancer Cell Lines ◽  
Induction Of Apoptosis ◽  
Brine Pool

Third Brine Pool in the Red Sea

Nature ◽  
1967 ◽  
Vol 213 (5077) ◽  
pp. 687-688 ◽  
Author(s):  
D. A. Ross ◽  
J. M. HUNT
Keyword(s):  
Red Sea ◽  
Brine Pool

scholarly journals Isolation and characterization of a heavy metal-resistant, thermophilic esterase from a Red Sea Brine Pool

2013 ◽  
Vol 3 (1) ◽  
Author(s):  
Yasmine M. Mohamed ◽  
Mohamed A. Ghazy ◽  
Ahmed Sayed ◽  
Amged Ouf ◽  
Hamza El-Dorry ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Red Sea ◽  
Thermophilic Esterase ◽  
Isolation And Characterization ◽  
Heavy Metal Resistant ◽  
Brine Pool

scholarly journals Diversity and distribution of eukaryotic microbes in and around a brine pool adjacent to the Thuwal cold seeps in the Red Sea

2014 ◽  
Vol 5 ◽  
Author(s):  
Yong Wang ◽  
Wei Peng Zhang ◽  
Hui Luo Cao ◽  
Chun Shum Shek ◽  
Ren Mao Tian ◽  
...  
Keyword(s):  
Red Sea ◽  
Cold Seeps ◽  
Eukaryotic Microbes ◽  
Brine Pool

scholarly journals Thermal Stability of a Mercuric Reductase from the Red Sea Atlantis II Hot Brine Environment as Analyzed by Site-Directed Mutagenesis

2018 ◽  
Vol 85 (3) ◽  
Author(s):  
Mohamad Maged ◽  
Ahmed El Hosseiny ◽  
Mona Kamal Saadeldin ◽  
Ramy K. Aziz ◽  
Eman Ramadan
Keyword(s):  
Thermal Stability ◽  
Red Sea ◽  
Structure Prediction ◽  
The Other ◽  
Site Directed Mutagenesis ◽  
Dimensional Structure ◽  
Parent Molecule ◽  
Mercuric Reductase ◽  
Strong Potential ◽  
Brine Pool

ABSTRACTThe lower convective layer (LCL) of the Atlantis II brine pool of the Red Sea is a unique environment in terms of high salinity, temperature, and high concentrations of heavy metals. Mercuric reductase enzymes functional in such extreme conditions could be considered a potential tool in the environmental detoxification of mercurial poisoning and might alleviate ecological hazards in the mining industry. Here, we constructed a mercuric reductase library from Atlantis II, from which we identified genes encoding two thermostable mercuric reductase (MerA) isoforms: one is halophilic (designated ATII-LCL) while the other is not (designated ATII-LCL-NH). The ATII-LCL MerA has a short motif composed of four aspartic acids (4D414–417) and two characteristic signature boxes that played a crucial role in its thermal stability. To further understand the mechanism behind the thermostability of the two studied enzymes, we mutated the isoform ATII-LCL-NH and found that the substitution of 2 aspartic acids (2D) at positions 415 and 416 enhanced the thermal stability, while other mutations had the opposite effect. The 2D mutant showed superior thermal tolerance, as it retained 81% of its activity after 10 min of incubation at 70°C. A three-dimensional structure prediction revealed newly formed salt bridges and H bonds in the 2D mutant compared to the parent molecule. To the best of our knowledge, this study is the first to rationally design a mercuric reductase with enhanced thermal stability, which we propose to have a strong potential in the bioremediation of mercurial poisoning.IMPORTANCEThe Red Sea is an attractive environment for bioprospecting. There are 25 brine-filled deeps in the Red Sea. The Atlantis II brine pool is the biggest and hottest of such hydrothermal ecosystems. We generated an environmental mercuric reductase library from the lowermost layer of the Atlantis II brine pool, in which we identified two variants of the mercuric reductase enzyme (MerA). One is the previously described halophilic and thermostable ATII-LCL MerA and the other is a nonhalophilic relatively less thermostable enzyme, designated ATII-LCL-NH MerA. We used the ATII-LCL-NH enzyme as a parent molecule to locate the amino acid residues involved in the noticeably higher thermotolerance of the homolog ATII-LCL MerA. Moreover, we designed a novel enzyme with superior thermal stability. This enzyme might have strong potential in the bioremediation of mercuric toxicity.

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