scholarly journals Characterization of glycosphingolipids from gastrointestinal stromal tumours

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Licínia Santos ◽  
Chunsheng Jin ◽  
Taťána Gazárková ◽  
Anders Thornell ◽  
Olov Norlén ◽  
...  
Keyword(s):  
Molecular Targets ◽  
Human Gastrointestinal Tract ◽  
Gastrointestinal Stromal Tumours ◽  
Detailed Characterization ◽  
Blood Group A ◽  
Group A ◽  
Layer Chromatography ◽  
Terminal Blood ◽  
New Molecular Targets

Abstract Gastrointestinal stromal tumours (GISTs) are the major nonepithelial neoplasms of the human gastrointestinal tract with a worldwide incidence between 11 and 15 per million cases annually. In this study the acid and non-acid glycosphingolipids of three GISTs were characterized using a combination of thin-layer chromatography, chemical staining, binding of carbohydrate recognizing ligands, and mass spectrometry. In the non-acid glycosphingolipid fractions of the tumors globotetraosylceramide, neolactotetraosylceramide, and glycosphingolipids with terminal blood group A, B, H, Lex, Lea, Ley and Leb determinants were found. The relative amounts of these non-acid compounds were different in the three tumour samples. The acid glycosphingolipid fractions had sulfatide, and the gangliosides GM3, GD3, GM1, Neu5Acα3neolactotetraosylceramide, GD1a, GT1b and GQ1b. In summary, we have characterized the glycosphingolipids of GISTs and found that the pattern differs in tumours from different individuals. This detailed characterization of glycosphingolipid composition of GISTs could contribute to recognition of new molecular targets for GIST treatment and sub-classification.

scholarly journals Isolation and characterization of novel glycolipids with blood group A-related structures: galactosyl-A and sialosylgalactosyl-A.

1987 ◽  
Vol 262 (29) ◽  
pp. 14228-14234
Author(s):  
H Clausen ◽  
S B Levery ◽  
E D Nudelman ◽  
M Stroud ◽  
M E Salyan ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Group A ◽  
Isolation And Characterization ◽  
Group A

Isolation and Characterization of a Blood Group A Substance-degrading α-N-Acetylgalactosaminidase from anAcremoniumsp.

1989 ◽  
Vol 53 (1) ◽  
pp. 111-120 ◽  
Author(s):  
Setsu Kadowaki ◽  
Takeshi Ueda ◽  
Kenji Yamamoto ◽  
Hidehiko Kumagai ◽  
Tatsurokuro Tochikura
Keyword(s):  
Blood Group ◽  
Blood Group A ◽  
Isolation And Characterization ◽  
Group A

Microarrays of 41 Human Tumor Cell Lines for the Characterization of New Molecular Targets: Expression Patterns of Cathepsin B and the Transferrin Receptor

Oncology ◽  
2006 ◽  
Vol 71 (1-2) ◽  
pp. 86-94 ◽  
Author(s):  
Gregory J. Wirth ◽  
Kathrin Schandelmaier ◽  
Victoria Smith ◽  
Angelika M. Burger ◽  
Heinz-Herbert Fiebig
Keyword(s):  
Transferrin Receptor ◽  
Cathepsin B ◽  
Expression Patterns ◽  
Molecular Targets ◽  
Human Tumor ◽  
Tumor Cell Lines ◽  
Human Tumor Cell ◽  
Human Tumor Cell Lines ◽  
New Molecular Targets

scholarly journals Structural characterization of neutral oligosaccharides with blood-group A and H activity isolated from bovine submaxillary mucin

1991 ◽  
Vol 279 (1) ◽  
pp. 95-103 ◽  
Author(s):  
A V Savage ◽  
S M D'Arcy ◽  
C M Donoghue
Keyword(s):  
Blood Group ◽  
Methylation Analysis ◽  
Molar Ratios ◽  
Blood Group A ◽  
Group A ◽  
Bovine Submaxillary Mucin ◽  
Type 3 ◽  
Blood Group H

In this study we investigated the structures of 11 neutral oligosaccharides released from bovine submaxillary mucin by alkaline borohydride treatment and isolated by h.p.l.c. One hexa-, one penta-, three tetra-, four tri- and two di-saccharides containing core types 1, 2, 3 or 4 were obtained. We report their structures, determined by a combination of one- and two-dimensional 1H n.m.r. spectroscopy at 270 MHz and methylation analysis involving g.l.c.-m.s., along with their approximate molar ratios. Only three of these oligosaccharides have previously been reported in this source. Of the new oligosaccharides, one contains the blood-group-A antigenic determinant, two contain the blood-group-H type 2 determinant, while another contains the blood-group-H type 3 determinant. The oligosaccharide GlcNAc beta (1→6)[GlcNAc beta (1→3)]GalNAcol, although previously found as a core structure, has been isolated here as a novel trisaccharide.

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