Immunochemical studies on blood groups. Purification and characterization of radioactive 3H-reduced di- to hexasaccharides produced by alkaline beta-elimination-borohydride 3H reduction of Smith degraded blood group A active glycoproteins.

Aim: To isolate, partially purify and characterize lectin from the seeds of Artocarpus species - using ammonium sulphate, gel-filtration and ion exchange chromatography. Methodology: ABO blood groups were screened for the assay of hemagglutinating property with seed lectins of both. heterophyllus and A. hirsutus. The seed lectins were extracted using suitable buffer system pH 7.0 and partially purified by gel filtration and ion exchange chromatographic techniques. Results and Discussion: Lectins were extracted from the defatted seed powders of A. heterophyllus and A. hirsutus. The extracts were used for optimization of assay buffer for hemagglutination activity and 50 mM Tris-HCl buffer, pH 8.8 containing 1 mM CaCl2 and 1 mM MnCl2 showed greater hemagglutinating property. Screening for optimum concentration of RBCs from different blood group (A, B, AB and O groups) indicated O group at 10 % concentration as ideal for the assay of hemagglutinating property with seed lectins of both A. heterophyllus and A. hirsutus. Conclusion: In this study, lectin from the seeds of Artocarpus heterophyllus and Artocarpus hirsutus were isolated and partially purified. The isolated lectins were characterized for their heamagglutination activity. Among the human blood types (A+, B+, AB+ and O+) used, all the blood groups showed agglutination while greater agglutination was observed with O+ blood group.

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Immunochemical Studies on Blood Groups. V. Further Characterization of Blood Group A and O Substances from Individual Hog Stomachs1a

Journal of the American Chemical Society ◽

10.1021/ja01201a031 ◽

1947 ◽

Vol 69 (9) ◽

pp. 2163-2167 ◽

Cited By ~ 30

Author(s):

Aaron Bendich ◽

Elvin A. Kabat ◽

Ada E. Bezer

Keyword(s):

Blood Group ◽

Blood Groups ◽

Blood Group A ◽

Group A

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Isolation and characterization of novel glycolipids with blood group A-related structures: galactosyl-A and sialosylgalactosyl-A.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)47927-6 ◽

1987 ◽

Vol 262 (29) ◽

pp. 14228-14234

Author(s):

H Clausen ◽

S B Levery ◽

E D Nudelman ◽

M Stroud ◽

M E Salyan ◽

...

Keyword(s):

Blood Group ◽

Blood Group A ◽

Isolation And Characterization ◽

Group A

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Distribution of ABO and Rh blood groups in Nepalese medical students: a report

Eastern Mediterranean Health Journal ◽

10.26719/2000.6.1.156 ◽

2000 ◽

Vol 6 (1) ◽

pp. 156-158

Author(s):

T. Pramanik ◽

S. Pramanik

Keyword(s):

Medical Students ◽

Blood Group ◽

Blood Groups ◽

Blood Group A ◽

Group A ◽

Group B ◽

Group Distribution

The frequencies of ABO and rhesus blood groups vary from one population to another. We studied blood group distribution in 120 Nepalese students; 34% were blood group A, 29% group B, 4% group AB and 32.5% group O. The frequency of Rh-negative blood was 3.33% and Rh-positive 96.66%

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Association of ABO blood groups with presentation and outcomes of confirmed SARS CoV-2 infection: A prospective study in the largest COVID-19 dedicated hospital in Bangladesh

PLoS ONE ◽

10.1371/journal.pone.0249252 ◽

2021 ◽

Vol 16 (4) ◽

pp. e0249252

Author(s):

Reaz Mahmud ◽

Mohammad Aftab Rassel ◽

Farhana Binte Monayem ◽

S. K. Jakaria Been Sayeed ◽

Md Shahidul Islam ◽

...

Keyword(s):

Blood Group ◽

Blood Groups ◽

Abo Blood Groups ◽

Duration Of Symptoms ◽

Presenting Symptoms ◽

Blood Group A ◽

Group I ◽

Significant Difference ◽

Group A ◽

Group Ii

Background Globally, studies have shown conflicting results regarding the association of blood groups with SARS CoV-2 infection. Objective To observe the association between ABO blood groups and the presentation and outcomes of confirmed COVID-19 cases. Design, setting, and participants This was a prospective cohort study of patients with mild-to-moderately severe COVID-19 infections who presented in the COVID-19 unit of Dhaka Medical College Hospital and were enrolled between 01 June and 25 August, 2020. Patients were followed up for at least 30 days after disease onset. We grouped participants with A-positive and A-negative blood groups into group I and participants with other blood groups into group II. Results The cohort included 438 patients; 52 patients were lost to follow-up, five died, and 381 completed the study. The prevalence of blood group A [144 (32.9%)] was significantly higher among COVID-19 patients than in the general population (p < 0.001). The presenting age [mean (SD)] of group I [42.1 (14.5)] was higher than that of group II [38.8 (12.4), p = 0.014]. Sex (p = 0.23) and co-morbidity (hypertension, p = 0.34; diabetes, p = 0.13) did not differ between the patients in groups I and II. No differences were observed regarding important presenting symptoms, including fever (p = 0.72), cough (p = 0.69), and respiratory distress (p = 0.09). There was no significant difference in the median duration of symptoms in the two group (12 days), and conversion to the next level of severity was observed in 26 (20.6%) and 36 patients (13.8%) in group I and II, respectively. However, persistent positivity of RT-PCR at 14 days of initial positivity was more frequent among the patients in group I [24 (19%)] than among those in group II [29 (11.1%)]. Conclusions The prevalence of blood group A was higher among COVID-19 patients. Although ABO blood groups were not associated with the presentation or recovery period of COVID-19, patients with blood group A had delayed seroconversion.

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IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS

Journal of Experimental Medicine ◽

10.1084/jem.85.6.685 ◽

1947 ◽

Vol 85 (6) ◽

pp. 685-699 ◽

Cited By ~ 34

Author(s):

Elvin A. Kabat ◽

Aaron Bendich ◽

Ada E. Bezer ◽

Sam M. Beiser

Keyword(s):

Blood Group ◽

Optical Rotation ◽

Maximal Activity ◽

Human Saliva ◽

Blood Groups ◽

Blood Group A ◽

Acetyl Content ◽

Group A ◽

Blood Group Substances ◽

Almost All

1. Blood group substances have been prepared from human saliva, stomach, and amniotic fluid from individuals of blood group A1 and A2. Several of the saliva samples were obtained from individuals shown to be heterozygous, A1O. 2. The purified blood group A substances from human sources were similar in nitrogen, glucosamine, reducing sugar, and acetyl content. The A1 and A2 substances differed in optical rotation. All of the human A samples were levorotatory while those from hog stomach were dextrorotatory. 3. By two immunochemical criteria the various human preparations could be shown to fall into distinct groups, with respect to purity. The best products showed maximal activity and almost all of their glucosamine was specifically precipitable by anti-A. These samples of human A substance were only about one-half as effective in precipitating antibody to hog A substance formed in man as was homologous hog A substance although the same total amount of antibody was precipitable by excess of either antigen. 4. Human blood group A1 substance was found to be antigenic in individuals of blood groups B and O but was not as good an antigen as hog A substance.

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