scholarly journals Towards mapping phenotypical traits in 18p− syndrome by array-based comparative genomic hybridisation and fluorescent in situ hybridisation

2006 ◽  
Vol 15 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Christian H Brenk ◽  
Eva-Christina Prott ◽  
Detlef Trost ◽  
Alexander Hoischen ◽  
Constanze Walldorf ◽  
...  
Keyword(s):  
In Situ Hybridisation ◽  
Comparative Genomic Hybridisation ◽  
Comparative Genomic ◽  
Fluorescent In Situ Hybridisation

Comparison of genomic abnormality in malignant mesothelioma by the site of origin

2014 ◽  
Vol 67 (12) ◽  
pp. 1038-1043 ◽  
Author(s):  
Maiko Takeda ◽  
Takahiko Kasai ◽  
Yasunori Enomoto ◽  
Masato Takano ◽  
Kohei Morita ◽  
...  
Keyword(s):  
Malignant Mesothelioma ◽  
In Situ Hybridisation ◽  
Homozygous Deletion ◽  
Comparative Genomic Hybridisation ◽  
Comparative Genomic ◽  
Fish Analysis ◽  
Fluorescence In Situ Hybridisation ◽  
Hybridisation Analysis ◽  
Genetic Events

AimsMalignant mesothelioma (MM) results from the accumulation of a number of acquired genetic events at the onset. In MM, the most frequent changes are losses in 9p21, 1p36, 22q12 and 14q32, and gains in 5p, 7p and 8q24 by comparative genomic hybridisation analysis. We have examined various genomic losses and gains in MM and benign mesothelial proliferation by fluorescence in situ hybridisation (FISH) analysis. 9p21 deletion was reported to be less frequent in peritoneal than in pleural MMs. This study analysed various genomic losses and gains in MM by the site of origin using FISH analysis.Materials and methodsWe performed FISH analysis using paraffin-embedded tissues from 54 cases (40 pleural and 14 peritoneal) of MMs and compared the frequency of genomic abnormality by the site of origin.Results9p21 deletion was shown in 34 of 40 cases (85%) of pleural MMs, and was less frequent in five of 14 cases (36%) of peritoneal MMs (p<0.001) by FISH analysis. By contrast, 5p15 and 7p12 amplification was more significantly frequent in peritoneal than in pleural MMs. No difference between the two sites of MM in other genes was found.Conclusions9p21 homozygous deletion assessed by FISH has been reported to be useful for differentiating MM from reactive mesothelial proliferation, but it should be noted that 9p21 deletion was less frequent in peritoneal MM. Our study suggests that the pathway of the genetic abnormality might vary between pleural and peritoneal MM.

Cytogenetic analysis of paediatric astrocytoma using comparative genomic hybridisation and fluorescence in-situ hybridisation

2010 ◽  
Vol 98 (3) ◽  
pp. 305-318 ◽  
Author(s):  
Samantha J. Ward ◽  
Katherine Karakoula ◽  
Kim P. Phipps ◽  
William Harkness ◽  
Richard Hayward ◽  
...  
Keyword(s):  
Cytogenetic Analysis ◽  
In Situ Hybridisation ◽  
Comparative Genomic Hybridisation ◽  
Comparative Genomic ◽  
Fluorescence In Situ Hybridisation

Validation of ROS1 by immunohistochemistry against fluorescent in situ hybridisation on cytology and small biopsy samples in a large teaching hospital

Cytopathology ◽  
2021 ◽  
Author(s):  
N. Narine ◽  
A.J. Wallace ◽  
J. Dore ◽  
S. O’Leary‐Jackson ◽  
H. Al‐Najjar ◽  
...  
Keyword(s):  
Teaching Hospital ◽  
In Situ Hybridisation ◽  
Fluorescent In Situ Hybridisation ◽  
Large Teaching Hospital
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