A new fingerprint method for sequence analysis of chromosomal translocations at the genomic DNA level

Dg2, a gene encoding a 34 kDa immunodominant antigen of Dirofilaria immitis was cloned and demonstrated to be specifically expressed in the larval stage. In this study, a newly constructed genomic DNA library was screened by hybridization with Dg2. One of the resulting positive clones was similar to Dg2 in the structure of its exonic regions but different in number, position, size and sequence of introns. This was designated DgK. Full-length cDNA was isolated using the rapid amplification of cDNA ends (RACE) method to study the transcript corresponding to DgK. Sequence analysis revealed that the mRNA corresponding to DgK is trans-spliced during post-transcriptional processing because the 5′ end of the amplified cDNA contains seven nucleotides of the nematode-spliced leader (SL) sequence.

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A 38 kb segment containing thecdc2 gene from the left arm of fission yeast chromosome II: sequence analysis and characterization of the genomic DNA and cDNAs encoded on the segment

Yeast ◽

10.1002/(sici)1097-0061(20000115)16:1<71::aid-yea505>3.0.co;2-5 ◽

2000 ◽

Vol 16 (1) ◽

pp. 71-80 ◽

Cited By ~ 2

Author(s):

Masayuki Machida ◽

Shuji Yamazaki ◽

Sumiko Kunihiro ◽

Toshihiro Tanaka ◽

Norihiro Kushida ◽

...

Keyword(s):

Sequence Analysis ◽

Fission Yeast ◽

Genomic Dna ◽

Yeast Chromosome ◽

Chromosome Ii

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Random sequence analysis of genomic DNA of an anaerobic, thermophilic, halophilic bacterium, Halothermothrix orenii

Extremophiles ◽

10.1007/s007920000174 ◽

2001 ◽

Vol 5 (1) ◽

pp. 61-69 ◽

Cited By ~ 26

Author(s):

Benjamin N. Mijts ◽

Bharat K.C. Patel

Keyword(s):

Sequence Analysis ◽

Genomic Dna ◽

Random Sequence ◽

Halophilic Bacterium

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Cloning and sequence analysis of calf cDNA and human genomic DNA encoding α-subunit precursor of muscle acetylcholine receptor

Nature ◽

10.1038/305818a0 ◽

1983 ◽

Vol 305 (5937) ◽

pp. 818-823 ◽

Cited By ~ 325

Author(s):

Masaharu Noda ◽

Yasuji Furutani ◽

Hideo Takahashi ◽

Mitsuyoshi Toyosato ◽

Tsutomu Tanabe ◽

...

Keyword(s):

Sequence Analysis ◽

Acetylcholine Receptor ◽

Genomic Dna ◽

Dna Encoding ◽

Α Subunit ◽

Human Genomic ◽

Human Genomic Dna

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DNA Sequencing and Comparative Sequence Analysis Reveal that the Escherichia coli Genomic DNA May Replace the Target DNA During Molecular Cloning: Evidence for the Erroneous Assembly of E. coli DNA into Database Sequences

Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology ◽

10.1016/s0305-0491(97)00175-2 ◽

1997 ◽

Vol 118 (2) ◽

pp. 333-339 ◽

Cited By ~ 1

Author(s):

Prakash K Jha ◽

Satyapriya Sarkar

Keyword(s):

Escherichia Coli ◽

Sequence Analysis ◽

Dna Sequencing ◽

Molecular Cloning ◽

Genomic Dna ◽

Comparative Sequence Analysis ◽

E Coli ◽

Comparative Sequence ◽

Target Dna

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Analyzing the MLL Recombinome.

Blood ◽

10.1182/blood.v104.11.4267.4267 ◽

2004 ◽

Vol 104 (11) ◽

pp. 4267-4267

Author(s):

Claus Meyer ◽

Bjoern Schneider ◽

Martin Reichel ◽

Sieglinde Angermuller ◽

Susanne Schnittger ◽

...

Keyword(s):

Genomic Dna ◽

Chromosomal Translocations ◽

Clinical Decisions ◽

Disease Entity ◽

Acute Leukemias ◽

Novel Technique ◽

Mll Gene ◽

Distinct Disease ◽

Distinct Disease Entity

Abstract Acute leukemias are frequently associated with specific chromosomal translocations of the human MLL gene. In general, MLL translocations define a distinct disease entity that needs to be diagnosed with precision to facilitate rapid clinical decisions. Here we present data about a new PCR based method that uses patient genomic DNA to identify any MLL fusion. Fourty different MLL translocations were successfully analyzed. We will present three novel MLL translocation partner genes and a new MLL deletion. The benefits of this novel technique for diagnosis and MRD analyses will be discussed. Supported by grant 2001.061.1 from the Wilhelm Sander foundation.

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