scholarly journals A transcription and translation-coupled DNA replication system using rolling-circle replication

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Yoshihiro Sakatani ◽  
Norikazu Ichihashi ◽  
Yasuaki Kazuta ◽  
Tetsuya Yomo
Keyword(s):  
Dna Replication ◽  
Rolling Circle Replication ◽  
Rolling Circle ◽  
Replication System

scholarly journals Rolling-Circle Replication of an Animal Circovirus Genome in a Theta-Replicating Bacterial Plasmid in Escherichia coli

2006 ◽  
Vol 80 (17) ◽  
pp. 8686-8694 ◽  
Author(s):  
Andrew K. Cheung
Keyword(s):  
Escherichia Coli ◽  
Dna Replication ◽  
Mammalian Cells ◽  
Unit Length ◽  
Release Mechanism ◽  
Rolling Circle Replication ◽  
Replication Process ◽  
Rolling Circle ◽  
Rep Protein ◽  
Bacterial Plasmid

ABSTRACT A bacterial plasmid containing 1.75 copies of double-stranded porcine circovirus (PCV) DNA in tandem (0.8 copy of PCV type 1 [PCV1], 0.95 copy of PCV2) with two origins of DNA replication (Ori) yielded three different DNA species when transformed into Escherichia coli: the input construct, a unit-length chimeric PCV1Rep/PCV2Cap genome with a composite Ori but lacking the plasmid vector, and a molecule consisting of the remaining 0.75 copy PCV1Cap/PCV2Rep genome with a different composite Ori together with the bacterial plasmid. Replication of the input construct was presumably via the theta replication mechanism utilizing the ColE1 Ori, while characteristics of the other two DNA species, including a requirement of two PCV Oris and the virus-encoded replication initiator Rep protein, suggest they were generated via the rolling-circle copy-release mechanism. Interestingly, the PCV-encoded Rep′ protein essential for PCV DNA replication in mammalian cells was not required in bacteria. The fact that the Rep′ protein function(s) can be compensated by the bacterial replication machinery to support the PCV DNA replication process echoes previous suggestions that circular single-stranded DNA animal circoviruses, plant geminiviruses, and nanoviruses may have evolved from prokaryotic episomal replicons.

scholarly journals Development of an in vitro bacteriophage N4 DNA replication system.

1986 ◽  
Vol 261 (23) ◽  
pp. 10506-10510
Author(s):  
J K Rist ◽  
M Pearle ◽  
A Sugino ◽  
L B Rothman-Denes
Keyword(s):  
Dna Replication ◽  
Replication System ◽  
Bacteriophage N4

scholarly journals PcrA Helicase and the Mechanism of Asymmetric Rolling Circle DNA Replication

2014 ◽  
Vol 106 (2) ◽  
pp. 72a
Author(s):  
Lesley Southerden ◽  
Claudia Arbore ◽  
Martin Webb
Keyword(s):  
Dna Replication ◽  
Asymmetric Rolling ◽  
Rolling Circle

scholarly journals Targeted Allele Replacement Mutagenesis of Corynebacterium pseudotuberculosis

2011 ◽  
Vol 77 (10) ◽  
pp. 3532-3535 ◽  
Author(s):  
Caray A. Walker ◽  
Willie Donachie ◽  
David G. E. Smith ◽  
Michael C. Fontaine
Keyword(s):  
Corynebacterium Pseudotuberculosis ◽  
Sequence Length ◽  
Homologous Sequence ◽  
Proof Of Concept ◽  
Rolling Circle Replication ◽  
Content Type ◽  
Rolling Circle ◽  
Allele Replacement ◽  
Marker Free ◽  
The Relationship

ABSTRACTA two-step allele replacement mutagenesis procedure, using a conditionally replicating plasmid, was developed to allow the creation of targeted, marker-free mutations inCorynebacterium pseudotuberculosis. The relationship between homologous sequence length and recombination frequency was determined, and enhanced plasmid excision was observed due to the rolling-circle replication of the mutagenesis vector. Furthermore, an antibiotic enrichment procedure was applied to improve the recovery of mutants. Subsequently, as proof of concept, a marker-free,cp40-deficient mutant ofC. pseudotuberculosiswas constructed.

Homogeneous Detection of Single Rolling Circle Replication Products

2004 ◽  
Vol 76 (2) ◽  
pp. 495-498 ◽  
Author(s):  
Gerhard A. Blab ◽  
Thomas Schmidt ◽  
Mats Nilsson
Keyword(s):  
Rolling Circle Replication ◽  
Rolling Circle
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