corynebacterium pseudotuberculosis
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Maria da Conceição Aquino de Sá ◽  
José Tadeu Raynal Rocha Filho ◽  
Maria Emilia Alcantara ◽  
Marcos da Costa Silva ◽  
Mariane Melo dos Santos ◽  

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12456
Wanderson Marques da Silva ◽  
Nubia Seyffert ◽  
Artur Silva ◽  
Vasco Azevedo

Background Corynebacterium pseudotuberculosis is a Gram-positive facultative intracellular pathogen and the etiologic agent of illnesses like caseous lymphadenitis in small ruminants, mastitis in dairy cattle, ulcerative lymphangitis in equines, and oedematous skin disease in buffalos. With the growing advance in high-throughput technologies, genomic studies have been carried out to explore the molecular basis of its virulence and pathogenicity. However, data large-scale functional genomics studies are necessary to complement genomics data and better understating the molecular basis of a given organism. Here we summarize, MS-based proteomics techniques and bioinformatics tools incorporated in genomic functional studies of C. pseudotuberculosis to discover the different patterns of protein modulation under distinct environmental conditions, and antigenic and drugs targets. Methodology In this study we performed an extensive search in Web of Science of original and relevant articles related to methods, strategy, technology, approaches, and bioinformatics tools focused on the functional study of the genome of C. pseudotuberculosis at the protein level. Results Here, we highlight the use of proteomics for understating several aspects of the physiology and pathogenesis of C. pseudotuberculosis at the protein level. The implementation and use of protocols, strategies, and proteomics approach to characterize the different subcellular fractions of the proteome of this pathogen. In addition, we have discussed the immunoproteomics, immunoinformatics and genetic tools employed to identify targets for immunoassays, drugs, and vaccines against C. pseudotuberculosis infection. Conclusion In this review, we showed that the combination of proteomics and bioinformatics studies is a suitable strategy to elucidate the functional aspects of the C. pseudotuberculosis genome. Together, all information generated from these proteomics studies allowed expanding our knowledge about factors related to the pathophysiology of this pathogen.

2021 ◽  
Vol 25 (1) ◽  
pp. 173-179
Majid N. Hussain

Light and electron microscopic examinations were performed on kidneys of sheep infected with caseous lymphadenitis ( CLA ) caused by Corynebacterium pseudotuberculosis Membranproliferative glomerulonephritis was demonstrated. There were irregular thickenings of the glomeruelar basement membrane and glomerular cell proliferation, in contrast, no such lesions were found in the control lambs, indicating that CLA play a role in inducing glomerulorephritis

2021 ◽  
pp. 63-68
Aline Melo da Silva ◽  
Bárbara Leão da Fonseca ◽  
Rógenes Ferreira Caetano ◽  
Samantha Tenório D‘amato Rosa ◽  
Rodrigo Antônio Torres Matos ◽  

2021 ◽  
Vol 10 (11) ◽  
pp. e509101119875
Maria Emília Alcântara ◽  
Antonio Pedro Fróes de Farias ◽  
Maria da Conceição Aquino de Sá ◽  
Soraya Castro Trindade ◽  
Roberto Meyer ◽  

The production of small ruminants is an important economic activity of the brazilian Northeast, but some diseases have a high prevalence in this region, such as caseous lymphadenitis (CL), caused by Corynebacterium pseudotuberculosis. The treatment of CL is often ineffective, which justifies the search of new active principles from plants, mainly of the region, to have an accessible treatment. For this reason, the present study was carried out to evaluate the in vitro antibacterial activity of Annona squamosa, Azadirachta indica, Allium sativum, Prosopis juliflora and Portulaca oleracea against C. pseudotuberculosis. Agar well diffusion assay (AWD) and broth microdilution - to determine minimum inhibitory concentration (MIC) and bactericidal concentration (MBC) - evaluated the antimicrobial activity. The highest antibacterial potential was obtained by ethanolic extracts of A. indica leaf (MIC 0,12 mg/mL for 2 strains), A. squamosa stalk (MIC 0,55 mg/mL for 3 strains) and shell (MIC 0,6 mg/mL for 3 strains). These extracts also presented the highest inhibition zone in AWD (30 mm, 38 mm and 32 mm, respectively). A. squamosa and A. indica have high antimicrobial potential against C. pseudotuberculosis.

2021 ◽  
pp. 106677
Jackeline Oliveira Costa ◽  
Thiago Doria Barral ◽  
Ricardo Dias Portela ◽  
Laura Nicole Filipin da Costa ◽  
Catarina Nunes Bittencourt ◽  

2021 ◽  
Vol 8 ◽  
Laerte Marlon Santos ◽  
Daniela Méria Rodrigues ◽  
Maurício Alcantara Kalil ◽  
Vasco Azevedo ◽  
Roberto Meyer ◽  

Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis in small ruminants, a chronic disease characterized by the development of granulomas in superficial and visceral lymph nodes as well as in several organs. An important characteristic of the infection with this bacterium is the formation of a biofilm and the absence of effective antibiotic therapy against the disease. From this scenario, the objective of this study was to evaluate the susceptibility of C. pseudotuberculosis to conventional antibiotics and to red, green, and brown propolis extracts obtained by the supercritical and ethanolic extraction methods as well as its activity in the bacterial biofilm. The results of the sensitivity test using antibiotics indicated a sensitivity of C. pseudotuberculosis strains to the antimicrobial agents. The ethanolic extract of green propolis and the supercritical red propolis extract showed the best antibacterial activities against planktonic C. pseudotuberculosis. A lower antimicrobial activity of the brown propolis extract was identified. Propolis extracts were effective in interfering with the formation of the C. pseudotuberculosis biofilm but had little activity on the consolidated biofilm. In conclusion, propolis extracts are more effective against C. pseudotuberculosis in the planktonic stage, being able to interfere with the formation of bacterial biofilm. However, the action of propolis extracts in a sessile and structured microbial biofilm is reduced.

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Daniela Droppa-Almeida ◽  
Caroline de Santana Ferreira ◽  
Ioná Brito ◽  
Sibele Borsuk ◽  
Jorge Alberto López Rodríguez ◽  

Corynebacterium pseudotuberculosis is the etiologic agent of Caseous Lymphadenitis (CLA), a disease leading to severe damage in sheep and goats farming due to the lack of serological diagnosis, treatment, and effective prophylaxis. In this context, several strategies in an attempt to discover new antigens to compose diagnosis assays or vaccines are fundamental. Therefore, this study aimed to use bioinformatics software to evaluate the critical chemical characteristics of unknown proteins of C. pseudotuberculosis by selecting them for heterologous expression in Escherichia coli. For this purpose, six protein sequences of ascorbate transporter subunit, UPF protein, MMPL family transporter, Ribonuclease, Iron ABC transporter domain-containing permease, and fimbrial subunit were obtained. In silico analyses were performed using amino acid sequences to access immunodominant epitopes and their antigenic and allergenic potential and physicochemical characterization. The expressed proteins were used as an antigen for serological diagnosis by ELISA. All proteins showed distinct immunodominant epitopes and potential antigenic characteristics. The only proteins expressed were PTS and Ribonuclease. In parallel, we expressed CP40 and all were used with ELISA antigen in 49 CLA positive sera and 26 CLA negative sera. The proteins alone showed 100% sensitivity and 96.2%, 92.3%, and 88.5% specificity for rPTS, rRibonuclease, and rCP40, respectively. When proteins were combined, they showed 100% sensitivity and 84.6%, 92.3%, 88.5%, and 92.3% specificity for rPTS/rCp40, rRibonuclease/rCP40, rPTS/rRibonuclease, and rPTS/rRibonuclease/rCP40, respectively. The results of this study show an excellent correlation of sensitivity and specificity with all proteins. None of the specificity values preclude the potential of rPTS, rRibonuclease, or rCP40 for use in ELISA diagnostic assays since the results of this work are superior to those of other studies on CLA diagnosis described in the literature.

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0252893
Abdelnasir Mohammed Adam Terab ◽  
Ghada El Derdiri Abdel Wahab ◽  
Hassan Zackaria Ali Ishag ◽  
Nasereldien Altaib Hussein Khalil ◽  
El Tigani Ahmed El Tigani-Asil ◽  

Caseous lymphadenitis (CLA) or pseudotuberculosis is a chronic zoonotic bacterial disease caused by Corynebacterium pseudotuberculosis, which affects livestock and humans. This study aimed to describe the pathology, bacteriology and confirm the identity of the pathogen by 16S rRNA gene sequencing in Camelus dromedarius. A total of 12 camels with suspected CLA in three regions of Abu Dhabi Emirate (Abu Dhabi, Al Ain and Al Dhafra), United Arab Emirate (UAE) were subjected to clinical and postmortem examinations from January 2015 to December 2020. Clinically, camels were emaciated and showed the presence of external caseous abscesses suggestive of CLA. Postmortem examination showed multiple abscesses of variable sizes with caseous material encapsulated by fibrous tissue in the liver, lungs, muscle, and lymph nodes. Following clinical and postmortem examination, blood, pus and different tissue samples were collected for subsequent analysis. Histopathological examination of all organs stained with Hematoxylin and Eosin (H&E) indicated a central caseo-necrotic core that was admixed with bacterial colonies and infiltration of chronic inflammatory cells, surrounded by a pyogenic membrane, and an outer fibrous connective tissue capsule. Bacterial culture identified the isolates of Corynebacterium pseudotuberculosis biotype ovis strain, and these isolates were shown to be sensitive to all antibiotics tested (penicillin, ampicillin, Co-trimoxazole, enrofloxacin and tetracycline). Moreover, the identity of the isolates was confirmed by partial sequencing of the 16S rRNA gene which showed a 100% identity to Corynebacterium pseudotuberculosis. Phylogenetic analysis based on 16S rRNA gene sequence clearly differentiates Corynebacterium pseudotuberculosis from other species of Corynebacterium. Briefly, this study provided the basic information for infection of Corynebacterium pseudotuberculosis in Camels and will help in controlling of this pathogen in the region.

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