scholarly journals ALB3 Insertase Mediates Cytochrome b6 Co-translational Import into the Thylakoid Membrane

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jarosław Króliczewski ◽  
Małgorzata Piskozub ◽  
Rafał Bartoszewski ◽  
Bożena Króliczewska
Keyword(s):  
Thylakoid Membrane ◽  
Cytochrome B6

Variable Composition of Cytochrome b6-f Particles

1976 ◽  
Vol 31 (9-10) ◽  
pp. 606-611 ◽  
Author(s):  
Hans-Joachim Lach ◽  
Peter Böger
Keyword(s):  
Cytochrome B ◽  
Thylakoid Membrane ◽  
Chemical Structure ◽  
Stoichiometric Composition ◽  
Variable Composition ◽  
Cyt B ◽  
Photosynthetic Membrane ◽  
Cytochrome B6 ◽  
Ionic Detergents

Abstract Cytochrome b6-f particles were prepared from chloroplasts of spinach and the heterokont alga Bumilleriopsis filiformis using digitonin in comparison with non-ionic detergents. The cytochrome b6-f particles are solubilized in different amounts according to chemical structure and HLB value of the detergent (see Griffin, J. Soc. Cosmet. Chem. 1, 311 [1949]). Also the ratio of the two cytochromes in the isolated particle depends on the detergent used. In case of spinach, it varies from Cyt b6: f = 1.5:1 to 4.9:1. These investigations do not strengthen the idea that a cytochrome b6-f complex with a stoichiometric composition is present in the photosynthetic membrane. Applica­tion of different detergents is a means for selective solubilization of thylakoid membrane com­ponents.

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