The determination of uric acid, particularly in avian excreta

The Analyst ◽  
1957 ◽  
Vol 82 (971) ◽  
pp. 110 ◽  
Author(s):  
Joseph Tinsley ◽  
T. Z. Nowakowski
Keyword(s):  
Uric Acid ◽  
Avian Excreta

scholarly journals Determination of Uric Acid in Avian Excreta by the Use of Uricase and Differential Spectrophotometry

1968 ◽  
Vol 47 (4) ◽  
pp. 1274-1277 ◽  
Author(s):  
W.J. Pudelkiewicz ◽  
M.W. Stutz ◽  
L.D. Matterson
Keyword(s):  
Uric Acid ◽  
Differential Spectrophotometry ◽  
Avian Excreta

Deproteinizing methods evaluated for determination of uric acid in serum by reversed-phase liquid chromatography with ultraviolet detection.

1987 ◽  
Vol 33 (8) ◽  
pp. 1427-1430 ◽  
Author(s):  
R Sakuma ◽  
T Nishina ◽  
M Kitamura
Keyword(s):  
Uric Acid ◽  
Liquid Chromatography ◽  
Perchloric Acid ◽  
High Performance ◽  
Reversed Phase ◽  
Precipitation Method ◽  
Zinc Hydroxide ◽  
Good Precision ◽  
Ultraviolet Detection

Abstract We evaluated six deproteinizing methods for determination of uric acid in serum by "high-performance" liquid chromatography with ultraviolet detection: those involving zinc hydroxide, sodium tungstate, trichloroacetic acid, perchloric acid, acetonitrile, and centrifugal ultrafiltration (with Amicon MPS-1 devices). We used a Toyosoda ODS-120A reversed-phase column. The mobile phase was sodium phosphate buffer (40 mmol/L, pH 2.2) containing 20 mL of methanol per liter. Absorbance of the eluate was monitored at 284 nm. The precipitation method with perchloric acid gave high recoveries of uric acid and good precision, and results agreed with those by the uricase-catalase method of Kageyama (Clin Chim Acta 1971;31:421-6).

scholarly journals THE DETERMINATION OF URIC ACID IN THE STUDY OF AVIAN NUTRITION

1931 ◽  
Vol 92 (1) ◽  
pp. 41-45
Author(s):  
J.L. St. John ◽  
Otto Johnson
Keyword(s):  
Uric Acid

scholarly journals COLORIMETRIC DETERMINATION OF URIC ACID

1922 ◽  
Vol 50 (1) ◽  
pp. 55-63 ◽  
Author(s):  
J.Lucien Morris ◽  
A.Garrard Macleod
Keyword(s):  
Uric Acid ◽  
Colorimetric Determination

scholarly journals Simultaneous Determination of Uric Acid and Xanthine Using a Poly(Methylene Blue) and Electrochemically Reduced Graphene Oxide Composite Film Modified Electrode

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Gen Liu ◽  
Wei Ma ◽  
Yan Luo ◽  
Deng-ming Sun ◽  
Shuang Shao
Keyword(s):  
Methylene Blue ◽  
Uric Acid ◽  
Graphene Oxide ◽  
Composite Film ◽  
Simultaneous Determination ◽  
Reduced Graphene Oxide ◽  
Modified Electrode ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

Poly(methylene blue) and electrochemically reduced graphene oxide composite film modified electrode (PMB-ERGO/GCE) was successfully fabricated by electropolymerization and was used for simultaneous determination of uric acid (UA) and xanthine (Xa). Based on the excellent electrocatalytic activity of PMB-ERGO/GCE, the electrochemical behaviors of UA and Xa were studied by cyclic voltammetry (CV) and square wave voltammetry (SWV). Two anodic sensitive peaks at 0.630 V (versus Ag/AgCl) for UA and 1.006 V (versus Ag/AgCl) for Xa were given by CV in pH 3.0 phosphate buffer. The calibration curves for UA and Xa were obtained in the range of 8.00 × 10−8~4.00 × 10−4 M and 1.00 × 10−7~4.00 × 10−4 M, respectively, by SWV. The detection limits for UA and Xa were3.00×10-8 M and5.00×10-8 M, respectively. Finally, the proposed method was applied to simultaneously determine UA and Xa in human urine with good selectivity and high sensitivity.

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