Thin-layer chromatography of phenyl thiohydantoin derivatives of amino acids on silica gel thin layers impregnated with zinc salts and some metal sulphates

The Analyst ◽  
1987 ◽  
Vol 112 (10) ◽  
pp. 1467 ◽  
Author(s):  
Ravi Bhushan ◽  
G. P. Reddy
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Thin Layers ◽  
Zinc Salts ◽  
Metal Sulphates ◽  
Layer Chromatography ◽  
Derivatives Of

Identification and Quantitation of Panthenol and Pantothenic Acid in Pharmaceutical Preparations by Thin-Layer Chromatography and Densitometry

1992 ◽  
Vol 75 (5) ◽  
pp. 898-901 ◽  
Author(s):  
Saurendra S Nag ◽  
Saroj K Das
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Benzyl Alcohol ◽  
Silica Gel ◽  
Pantothenic Acid ◽  
Pharmaceutical Preparations ◽  
Ninhydrin Reaction ◽  
Layer Chromatography

Abstract A rapid, simple, and specific thin-layer chromatographic (TLC) method has been developed for the estimation of panthenol and pantothenic acid in pharmaceutical preparations containing other vitamins, amino acids, syrups, enzymes, etc. The vitamin is extracted with ethanol (tablets and capsules) or benzyl alcohol (liquid oral preparations) and isolated from other ingredients by TLC on silica gel with isopropanol-water (85 + 15, v/v) as developing solvent. β-Alanine (pantothenate) or β-alanol (panthenol) is liberated by heating for 20 min at 160°C. The liberated amines are visualized by ninhydrin reaction and estimated by spectrodensitometry at 490 nm. Recoveries for panthenol and pantothenic acid were 99.8 ± 2.25% and 100.2 ± 1.7%, respectively.

scholarly journals The reaction of iodobenzene-p-sulphonyl chloride (pipsyl chloride) with certain amino acids and peptides, and with insulin

1967 ◽  
Vol 102 (3) ◽  
pp. 815-824 ◽  
Author(s):  
J. C. Fletcher
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Acid Hydrolysis ◽  
Thin Layer Chromatography ◽  
Cysteic Acid ◽  
Buffer System ◽  
The Common ◽  
Layer Chromatography ◽  
Derivatives Of

1. A system of separation using buffered Celite columns is described that enables the pipsyl derivatives of most of the common amino acids to be separated. 2. The reaction of pipsyl chloride with several amino acids not included in previous studies has been investigated. In particular, knowledge of the acid-soluble pipsyl derivatives of arginine, histidine, lysine, tyrosine and cysteic acid has been extended. 3. Reproducible factors have been obtained that enable corrections to be applied for the breakdown of pipsylamino acids on acid hydrolysis. 4. The reaction of pipsyl chloride with peptides has been studied under various conditions. 5. The extent of the reaction between pipsyl chloride and insulin depends on the nature of the solvent-buffer system, and under the best conditions so far found is about 75% complete. 6. In an Appendix, the separation of pipsylamino acids by thin-layer chromatography is described.

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