Native and fluorescent dye-dependent single-DNA molecule microchip dynamics as measured by differential interference contrast microscopy

2011 ◽  
Vol 47 (32) ◽  
pp. 9137 ◽  
Author(s):  
Doori Oh ◽  
Seungah Lee ◽  
Seong Ho Kang
Keyword(s):  
Fluorescent Dye ◽  
Differential Interference Contrast ◽  
Differential Interference Contrast Microscopy ◽  
Dna Molecule ◽  
Contrast Microscopy ◽  
Interference Contrast Microscopy ◽  
Single Dna Molecule

scholarly journals Optical sectioning in differential interference contrast microscopy

2009 ◽  
Vol 282 (16) ◽  
pp. 3223-3230 ◽  
Author(s):  
Atsushi Noguchi ◽  
Hiroshi Ishiwata ◽  
Masahide Itoh ◽  
Toyohiko Yatagai
Keyword(s):  
Differential Interference Contrast ◽  
Differential Interference Contrast Microscopy ◽  
Optical Sectioning ◽  
Contrast Microscopy ◽  
Interference Contrast Microscopy

scholarly journals Minus-end capture of preformed kinetochore fibers contributes to spindle morphogenesis

2003 ◽  
Vol 160 (5) ◽  
pp. 671-683 ◽  
Author(s):  
Alexey Khodjakov ◽  
Lily Copenagle ◽  
Michael B. Gordon ◽  
Duane A. Compton ◽  
Tarun M. Kapoor
Keyword(s):  
Three Dimensional ◽  
Differential Interference Contrast ◽  
Differential Interference Contrast Microscopy ◽  
Spindle Formation ◽  
Capture Mechanism ◽  
Contrast Microscopy ◽  
Interference Contrast Microscopy ◽  
Kinesin Eg5 ◽  
Mitotic Kinesin Eg5 ◽  
Dependent Mechanism

Near-simultaneous three-dimensional fluorescence/differential interference contrast microscopy was used to follow the behavior of microtubules and chromosomes in living α-tubulin/GFP-expressing cells after inhibition of the mitotic kinesin Eg5 with monastrol. Kinetochore fibers (K-fibers) were frequently observed forming in association with chromosomes both during monastrol treatment and after monastrol removal. Surprisingly, these K-fibers were oriented away from, and not directly connected to, centrosomes and incorporated into the spindle by the sliding of their distal ends toward centrosomes via a NuMA-dependent mechanism. Similar preformed K-fibers were also observed during spindle formation in untreated cells. In addition, upon monastrol removal, centrosomes established a transient chromosome-free bipolar array whose orientation specified the axis along which chromosomes segregated. We propose that the capture and incorporation of preformed K-fibers complements the microtubule plus-end capture mechanism and contributes to spindle formation in vertebrates.

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