Synthetic fluorescent probes capable of selective recognition of 3′-overhanging nucleotides for siRNA delivery imaging

2015 ◽  
Vol 51 (8) ◽  
pp. 1421-1424 ◽  
Author(s):  
Takaya Sato ◽  
Yusuke Sato ◽  
Kenta Iwai ◽  
Shusuke Kuge ◽  
Seiichi Nishizawa ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Fluorescent Probes ◽  
Peptide Nucleic Acid ◽  
Sirna Delivery ◽  
Selective Recognition ◽  
Thiazole Orange ◽  
Accurate Analysis

Peptide nucleic acid (PNA)–thiazole orange (TO) conjugates are developed as fluorescent probes capable of selective recognition of 3′-overhanging nucleotides of siRNAs for an accurate analysis of the siRNA delivery process.

Real time RNA transcription monitoring by Thiazole Orange (TO)-conjugated Peptide Nucleic Acid (PNA) probes: norovirus detection

2011 ◽  
Vol 7 (5) ◽  
pp. 1684 ◽  
Author(s):  
Alessandro Tonelli ◽  
Tullia Tedeschi ◽  
Andrea Germini ◽  
Stefano Sforza ◽  
Roberto Corradini ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Peptide Nucleic Acid ◽  
Thiazole Orange ◽  
Rna Transcription

scholarly journals Thiazole orange-conjugated peptide nucleic acid for fluorescent detection of specific DNA sequences and site-selective photodamage

RSC Advances ◽  
2014 ◽  
Vol 4 (108) ◽  
pp. 63533-63538 ◽  
Author(s):  
Makiko Tanaka ◽  
Narumi Shigi ◽  
Jun Sumaoka ◽  
Makoto Komiyama
Keyword(s):  
Nucleic Acid ◽  
Dna Sequences ◽  
Peptide Nucleic Acid ◽  
Fluorescent Detection ◽  
Thiazole Orange ◽  
Site Selective

Conjugates of thiazole orange (TO) with a pseudo-complementary peptide nucleic acid (pcPNA) functioned as (i) fluorescent detector of specific DNA and (ii) site-selective photodamage inducer through generation of 1O2.

scholarly journals Chloro-Substituted Naphthyridine Derivative and Its Conjugate with Thiazole Orange for Highly Selective Fluorescence Sensing of an Orphan Cytosine in the AP Site-Containing Duplexes

2020 ◽  
Vol 10 (12) ◽  
pp. 4133
Author(s):  
Chun-xia Wang ◽  
Yusuke Sato ◽  
Takashi Sugimoto ◽  
Norio Teramae ◽  
Seiichi Nishizawa
Keyword(s):  
Nucleic Acid ◽  
Sequence Analysis ◽  
Fluorescent Probes ◽  
Dna Duplexes ◽  
Thiazole Orange ◽  
Fluorescence Sensing ◽  
Binding Selectivity ◽  
Target Dna ◽  
Fluorescence Signaling ◽  
Ap Site

Fluorescent probes with the binding selectivity to specific structures in DNAs or RNAs have gained much attention as useful tools for the study of nucleic acid functions. Here, chloro-substituted 2-amino-5,7-dimethyl-1,8-naphthyridine (ClNaph) was developed as a strong and highly selective binder for target orphan cytosine opposite an abasic (AP) site in the DNA duplexes. ClNaph was then conjugated with thiazole orange (TO) via an alkyl spacer (ClNaph–TO) to design a light-up probe for the detection of cytosine-related mutations in target DNA. In addition, we found the useful binding and fluorescence signaling of the ClNaph–TO conjugate to target C in AP site-containing DNA/RNA hybrid duplexes with a view toward sequence analysis of microRNAs.

scholarly journals Fluorescence Imaging of siRNA Delivery by Peptide Nucleic Acid-based Probe

2015 ◽  
Vol 31 (4) ◽  
pp. 315-320 ◽  
Author(s):  
Takaya SATO ◽  
Yusuke SATO ◽  
Kenta IWAI ◽  
Shusuke KUGE ◽  
Norio TERAMAE ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Fluorescence Imaging ◽  
Peptide Nucleic Acid ◽  
Sirna Delivery
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