Fabrication and characterization of the nano-composite of whey protein hydrolysate chelated with calcium

2015 ◽  
Vol 6 (3) ◽  
pp. 816-823 ◽  
Author(s):  
Cai Xixi ◽  
Zhao Lina ◽  
Wang Shaoyun ◽  
Rao Pingfan
Keyword(s):  
Calcium Ions ◽  
Calcium Binding ◽  
Calcium Absorption ◽  
Protein Hydrolysate ◽  
Carboxyl Groups ◽  
Carbonyl Groups ◽  
Nano Composite ◽  
Fabrication And Characterization ◽  
Whey Protein Hydrolysate

The chelation of calcium ions to WPH caused molecular folding and aggregation leading to the formation of a WPH-calcium chelate of nanoparticle size, and the principal sites of calcium-binding corresponded to the carboxyl groups and carbonyl groups of WPH. The WPH-calcium chelate could significantly improve calcium absorption at the intestinal level.

Preparation and characterization of whey protein hydrolysate-Zn complexes

2019 ◽  
Vol 14 (1) ◽  
pp. 254-261 ◽  
Author(s):  
Rongchun Wang ◽  
Shenghua He ◽  
Yifan Xuan ◽  
Cuilin Cheng
Keyword(s):  
Whey Protein ◽  
Protein Hydrolysate ◽  
Whey Protein Hydrolysate

Design, fabrication, and characterization of low-power gas sensors based on organic-inorganic nano-composite

2017 ◽  
Vol 42 ◽  
pp. 284-292 ◽  
Author(s):  
Ayah F.S. Abu-Hani ◽  
Falah Awwad ◽  
Yaser E. Greish ◽  
Ahmad I. Ayesh ◽  
Saleh T. Mahmoud
Keyword(s):  
Low Power ◽  
Gas Sensors ◽  
Nano Composite ◽  
Fabrication And Characterization

Purification and characterisation of a glutamic acid-containing peptide with calcium-binding capacity from whey protein hydrolysate

2015 ◽  
Vol 82 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Shun-Li Huang ◽  
Li-Na Zhao ◽  
Xixi Cai ◽  
Shao-Yun Wang ◽  
Yi-Fan Huang ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Whey Protein ◽  
Calcium Binding ◽  
Protein Hydrolysate ◽  
Binding Capacity ◽  
Reversed Phase ◽  
Exchange Chromatography ◽  
Binding Peptide ◽  
Whey Protein Hydrolysate

The bioavailability of dietary ionised calcium is affected by intestinal basic environment. Calcium-binding peptides can form complexes with calcium to improve its absorption and bioavailability. The aim of this study was focused on isolation and characterisation of a calcium-binding peptide from whey protein hydrolysates. Whey protein was hydrolysed using Flavourzyme and Protamex with substrate to enzyme ratio of 25 : 1 (w/w) at 49 °C for 7 h. The calcium-binding peptide was isolated by DEAE anion-exchange chromatography, Sephadex G-25 gel filtration and reversed phase high-performance liquid chromatography (RP-HPLC). A purified peptide of molecular mass 204 Da with strong calcium binding ability was identified on chromatography/electrospray ionisation (LC/ESI) tandem mass spectrum to be Glu-Gly (EG) after analysis and alignment in database. The calcium binding capacity of EG reached 67·81 μg/mg, and the amount increased by 95% compared with whey protein hydrolysate complex. The UV and infrared spectrometer analysis demonstrated that the principal sites of calcium-binding corresponded to the carboxyl groups and carbonyl groups of glutamic acid. In addition, the amino group and peptide amino are also the related groups in the interaction between EG and calcium ion. Meanwhile, the sequestered calcium percentage experiment has proved that EG-Ca is significantly more stable than CaCl2 in human gastrointestinal tract in vitro. The findings suggest that the purified dipeptide has the potential to be used as ion-binding ingredient in dietary supplements.

Fabrication and characterization of ABS nano composite reinforced by nano sized alumina particulates

2009 ◽  
Vol 13 (2) ◽  
pp. 133-149 ◽  
Author(s):  
Vivek Kumar ◽  
J. RamKumar ◽  
S. Aravindan ◽  
S. K. Malhotra ◽  
K. Vijai ◽  
...  
Keyword(s):  
Nano Composite ◽  
Fabrication And Characterization

Calcium-Binding Capacity of Wheat Germ Protein Hydrolysate and Characterization of Peptide–Calcium Complex

2013 ◽  
Vol 61 (31) ◽  
pp. 7537-7544 ◽  
Author(s):  
Feng-Ru Liu ◽  
Li Wang ◽  
Ren Wang ◽  
Zheng-Xing Chen
Keyword(s):  
Calcium Binding ◽  
Wheat Germ ◽  
Protein Hydrolysate ◽  
Binding Capacity

Fabrication and characterization of Sialon–Si3N4 graded nano-composite ceramic tool materials

2011 ◽  
Vol 42 (7) ◽  
pp. 1813-1820 ◽  
Author(s):  
G.M. Zheng ◽  
J. Zhao ◽  
Y.H. Zhou ◽  
Z.J. Gao ◽  
X.B. Cui ◽  
...  
Keyword(s):  
Composite Ceramic ◽  
Ceramic Tool ◽  
Nano Composite ◽  
Tool Materials ◽  
Fabrication And Characterization
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