scholarly journals Effect of adhesion and chemokine presentation on T-lymphocyte haptokinesis

2014 ◽  
Vol 6 (9) ◽  
pp. 862-873 ◽  
Author(s):  
George A. Dominguez ◽  
Daniel A. Hammer
Keyword(s):  
T Lymphocytes ◽  
T Lymphocyte ◽  
Random Motility ◽  
Human T Lymphocytes

The random motility of human T-lymphocytes was measured on microcontact printed surfaces containing ICAM-1 and VCAM-1, and the additional effects of the chemokines CCL21 and CCL19 were investigated. This image shows the morphology of human T-lymphocytes on ICAM-1 substrates in the presence of immobilized CCL21, immunostained for actin (in red) and α-tubulin (in green).

scholarly journals Activation of c-myb expression by phytohemagglutinin stimulation in normal human T lymphocytes.

1985 ◽  
Vol 5 (10) ◽  
pp. 2874-2877 ◽  
Author(s):  
G Torelli ◽  
L Selleri ◽  
A Donelli ◽  
S Ferrari ◽  
G Emilia ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
T Lymphocytes ◽  
T Lymphocyte ◽  
Histone H3 ◽  
Lymphocyte Subset ◽  
Continuous Growth ◽  
Human T Lymphocytes ◽  
Normal Human ◽  
T Lymphocyte Subset

The expression of c-myb in normal human T lymphocytes directly derived from a normal subject and not adapted to continuous growth in culture was found to be markedly increased after phytohemagglutinin stimulation. In the same cells, the expression of c-myc mRNA is a much earlier event compared with the appearance of c-myb mRNA, which takes place soon after that of histone H3 mRNA. The increase in c-myb expression was not due to a particular T-lymphocyte subset, as shown by in situ hybridization assays.

scholarly journals Comparative Studies of the AgNORS Motifs in Phytohemagglutinin-Stimulated Human T-Lymphocytes with T-Lymphocyte Subgroups

2012 ◽  
Vol 34 (3) ◽  
pp. 132-136
Author(s):  
Nazmiye Bitgen ◽  
Hamiyet Donmez- Altuntas ◽  
Zuhal Hamurcu ◽  
Halil Demirtas ◽  
Figen Ozturk
Keyword(s):  
T Lymphocytes ◽  
T Lymphocyte ◽  
Comparative Studies ◽  
Human T Lymphocytes

FcγRIIIa (CD16) Induction on Human T Lymphocytes and CD16pos T-Lymphocyte Amplification

2011 ◽  
Vol 34 (7) ◽  
pp. 542-549 ◽  
Author(s):  
Béatrice Clémenceau ◽  
Régine Vivien ◽  
Emilie Debeaupuis ◽  
Julie Esbelin ◽  
Charlotte Biron ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
T Lymphocyte ◽  
Human T Lymphocytes

scholarly journals Genome-Wide Transcriptional and Functional Analysis of Human T Lymphocytes Treated with Benzo[α]pyrene

2018 ◽  
Vol 19 (11) ◽  
pp. 3626 ◽  
Author(s):  
Marie Liamin ◽  
Hélène Le Mentec ◽  
Bertrand Evrard ◽  
Laurence Huc ◽  
Frédéric Chalmel ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
T Lymphocyte ◽  
Cytochromes P450 ◽  
Enrichment Analysis ◽  
Functional Enrichment Analysis ◽  
Functional Enrichment ◽  
Lymphocyte Migration ◽  
Human T Lymphocytes ◽  
Aryl Hydrocarbon ◽  
Genes Encoding

Polycyclic aromatic hydrocarbons (PAHs) are widely distributed environmental contaminants, known to affect T lymphocytes. However, the molecular targets and pathways involved in their immunotoxic effects in human T lymphocytes remain unknown. Here, we analyzed the gene expression profile of primary human T lymphocytes treated with the prototypical PAH, benzo[α]pyrene (B[α]P), using a microarray-based transcriptome analysis. After a 48 h exposure to B[α]P, we identified 158 genes differentially expressed in T lymphocytes, including not only genes well-known to be affected by PAHs such as the cytochromes P450 (CYP) 1A1 and 1B1, but also others not previously shown to be targeted by B[α]P such as genes encoding the gap junction beta (GJB)-2 and 6 proteins. Functional enrichment analysis revealed that these candidates were significantly associated with the aryl hydrocarbon (AhR) and interferon (IFN) signaling pathways; a marked alteration in T lymphocyte recruitment was also observed. Using functional tests in transwell migration experiments, B[α]P was then shown to significantly decrease the chemokine (C-X-C motif) ligand 12-induced chemotaxis and transendothelial migration of T lymphocytes. In total, this study opens the way to unsuspected responsive pathway of interest, i.e., T lymphocyte migration, thus providing a more thorough understanding of the molecular basis of the immunotoxicity of PAHs.

Plasma membrane subdomain partitioning of Lck in primary human T lymphocytes

2010 ◽  
Vol 88 (4) ◽  
pp. 487-496 ◽  
Author(s):  
Claudine Irles ◽  
Joel Arias-Martinez ◽  
José Guzmán-Bárcenas ◽  
Alicia Ortega
Keyword(s):  
Plasma Membrane ◽  
T Lymphocytes ◽  
T Lymphocyte ◽  
Human Peripheral Blood ◽  
Discrete Distribution ◽  
Cell Receptor ◽  
Negative Regulator ◽  
Resting Cells ◽  
Human T Lymphocytes ◽  
Tcr Activation

Uncovering the plasma membrane distribution of tyrosine kinase Lck is crucial to understanding T lymphocyte triggering. Several studies of Lck species partitioning have given contradictory results. We decided to re-address this point by using phospho-specific antibodies to characterize active and inactive Lck partitioning in raft and non-raft membranes from primary human peripheral blood T lymphocytes. We show that most inactive Lck was localized in rafts and was associated with nearly all CD4 coreceptors and its negative regulator Csk in resting cells, while T cell receptor (TCR) engagement promoted a sustained dephosphorylation of inactive Lck. In contrast, active Lck had a more discrete distribution interacting with only a small number of CD4 coreceptors, and the kinase showed a rapid and short phosphorylation after TCR triggering. The differences in distribution and kinetics may be related to T lymphocyte signalling threshold modulation by Lck species and suggest how TCR triggering is first initiated. This study furthers our knowledge of the TCR activation model in primary human T lymphocytes.

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