3,6-Bis(1-methyl-4-vinylpyridinium)-carbazole diiodide as a marker for tracking living neural stem/precursor cells

2015 ◽  
Vol 3 (10) ◽  
pp. 2067-2074 ◽  
Author(s):  
Yi-Chen Li ◽  
Jyh-Horng Wang ◽  
Li-Kai Tsai ◽  
Yun-An Chen ◽  
Ta-Chau Chang ◽  
...  

A novel compound, 3,6-bis(1-methyl-4-vinylpyridinium)-carbazole diiodide, is used as a marker for distinguishing living neural stem/precursor cells (NSPCs) from adipose-derived stem cells (ADSCs) and bone marrow mesenchymal stem cells (BMMSCs).

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4244-4244
Author(s):  
Rei Ogawa ◽  
Juri Fujimura ◽  
Keiko Hirakawa ◽  
Kyoko Uekusa ◽  
Kohtaro Yuta ◽  
...  

Abstract Background: Nuclear magnetic resonance (NMR) -based metabolomics has been suggested to be useful for exhaustive analysis of metabolic pathways of cells or tissues. We report our study on bone marrow mesenchymal stem cells (BSCs) and adipose-derived stem cells (ASCs) using NMR-based metabolomics. Materials and Methods: Five-week-old Wister rats were used for this study. BSCs were harvested from the femur, ASCs from inguinal fat pads, and control fibroblasts from the abdominal dermis. The cells were cultured in DMEM with 10% fetal bovine serum and harvested after two passages of the subculture. Then, the cells were subjected to freezing in liquid nitrogen and crushed to extract the aqueous metabolites. 1H-NMR spectra was measured and analyzed by a computer software (Alice2 for metabolome™ and ADMEWORKS/ModelbuilderTM). Results: BSCs, ASCs and fibroblasts were clearly separated into three groups on a principal component analysis (PCA) plot. Conclusion: ASCs, BSCs and fibroblasts were considered to have different metabolic activities, and NMR-based metabolomics will henceforth be useful for the detection, analysis, and characterization of various kinds of stem cells.


Cell Research ◽  
2008 ◽  
Vol 18 (S1) ◽  
pp. S76-S76 ◽  
Author(s):  
Xuerong Sun ◽  
Jian Ge ◽  
Ruzhang Jiang ◽  
Shan Duan ◽  
Ge Song ◽  
...  

2013 ◽  
Author(s):  
Melo Ocarino Natalia de ◽  
Silvia Silva Santos ◽  
Lorena Rocha ◽  
Juneo Freitas ◽  
Reis Amanda Maria Sena ◽  
...  

2014 ◽  
Author(s):  
Reis Amanda Maria Sena ◽  
Freitas Silva Juneo de ◽  
Silvia Silva Santos ◽  
Rogeria Serakides ◽  
Melo Ocarino Natalia de

2013 ◽  
Vol 28 (3) ◽  
pp. 224-231
Author(s):  
Zhenhua REN ◽  
Jiayin WANG ◽  
Shuyan WANG ◽  
Ying ZHANG ◽  
Chunlin ZOU ◽  
...  

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