Metabolic Analysis of Bone Marrow Mesenchymal Stem Cells and Adipose-Derived Stem Cells Using NMR-Based Metabolomics.

Abstract Background: Nuclear magnetic resonance (NMR) -based metabolomics has been suggested to be useful for exhaustive analysis of metabolic pathways of cells or tissues. We report our study on bone marrow mesenchymal stem cells (BSCs) and adipose-derived stem cells (ASCs) using NMR-based metabolomics. Materials and Methods: Five-week-old Wister rats were used for this study. BSCs were harvested from the femur, ASCs from inguinal fat pads, and control fibroblasts from the abdominal dermis. The cells were cultured in DMEM with 10% fetal bovine serum and harvested after two passages of the subculture. Then, the cells were subjected to freezing in liquid nitrogen and crushed to extract the aqueous metabolites. 1H-NMR spectra was measured and analyzed by a computer software (Alice2 for metabolome™ and ADMEWORKS/ModelbuilderTM). Results: BSCs, ASCs and fibroblasts were clearly separated into three groups on a principal component analysis (PCA) plot. Conclusion: ASCs, BSCs and fibroblasts were considered to have different metabolic activities, and NMR-based metabolomics will henceforth be useful for the detection, analysis, and characterization of various kinds of stem cells.

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Abstract 2838: Bone Marrow Mesenchymal Stem Cells Differentiated into Beating Cardiomyocytes In Vivo and Improved Myocardial Function

Circulation ◽

10.1161/circ.116.suppl_16.ii_631-c ◽

2007 ◽

Vol 116 (suppl_16) ◽

Author(s):

Tong Wang ◽

Wanchun Tang ◽

Shijie Sun ◽

Min-shan Tsai ◽

Max Harry Weil

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Myocardial Function ◽

Phosphate Buffer Solution ◽

Sprague Dawley ◽

Buffer Solution ◽

Marrow Mesenchymal Stem Cells ◽

And Control

Background: In settings of heart failure, infusion of bone marrow mesenchymal stem cells (MSCs) improves myocardial function both in experimental and clinical studies. The mechanism by which MSCs improve myocardial function remains unknown. Hypothesis: MSCs may differentiate into beating myocytes in vivo. The contractility of these cells is comparable with those of myocytes. Methods: A thoracotomy was performed in 10 male Sprague-Dawley rats, weighing 350 – 450g. Myocardial infarction was induced by ligation of the left anterior descending artery (LAD). One week later, animals were randomized to receive 5×10 6 MSCs marked with PKH26 in phosphate buffer solution (PBS) or as a PBS bolus injection into local infarcted myocardium. Six weeks after the MSCs or PBS injection, the hearts were harvested and digested with collagease type II and single cardiomyocytes were obtained. PKH26 labeled myocytes differentiating from MSCs were observed with a microscope Olympus I×71. The contractility of labeled and unlabeled beating cells in MSCs-treated animals was compared. The contractility of unlabeled myocytes was compared between MSCs-treated and control groups. Result: The beating fluorescent labeled myocytes can be found in MSCs-treated animals [(1.2±0.4) ×10 6 ] and contractility of these cells were the same as that of unlabeled beating myocytes (Table 1 ). The contractility of unlabeled myocytes, however, was significantly better in MSCs-treated animals. Conclusion: MSCs could differentiate into the beating myocytes. However, this may not be the sole mechanism of improved myocardial function. Table 1 Cells contractility (%)

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miR-1 Activates NF-κB to Promote the Differentiation of Bone Marrow Mesenchymal Stem Cells in Mouse Models of Glioma

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2021.2694 ◽

2021 ◽

Vol 11 (7) ◽

pp. 1327-1332

Author(s):

Long Zhou ◽

Kui Wang ◽

Meixia Liu ◽

Wen Wei ◽

Liu Liu ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Western Blot ◽

Mouse Models ◽

Cell Apoptosis ◽

Bone Diseases ◽

Control Group ◽

Marrow Mesenchymal Stem Cells ◽

And Control

NF-κB activation and its abnormal expression are involved in the progression of glioma. miRNA plays a crucial role in bone diseases. The role of NF-κB is becoming more and more important. The purpose of this study is to explore the mechanism by how miR-1 regulates NF-κB signaling. C57 glioma mouse models were divided into osteoporosis (OP) group and control group. qPCR was used to measure miR-1 levels in OP and control mice. Bone marrow mesenchymal stem cells (BMSCs) were cultured and transfected with miR-1 specific siRNA to establish miR-1 knockout cell model followed by analysis of cell apoptosis, expression of NF-κB signaling molecules by western blot. qPCR results showed that miR-1 levels in OP mice were significantly reduced compared to control mice. A large number of siRNA particles were observed in transfected BMSCs under a fluorescence microscope. qPCR results showed that siRNA transfection significantly suppressed miR-1, indicating successful transfection. Flow cytometry revealed significant differences in cell apoptosis between miR-1 siRNA group and the NC group. Western blot indicated miR-1 promoted BMSCs differentiation via NF-κB mediated up-regulation of ALP activity. The expression of miR-1 is low in BMSCs of mice with glioma. In addition, BMSCs differentiation is enhanced by NF-κB activation via up-regulating miR-1.

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3,6-Bis(1-methyl-4-vinylpyridinium)-carbazole diiodide as a marker for tracking living neural stem/precursor cells

Journal of Materials Chemistry B ◽

10.1039/c4tb01903b ◽

2015 ◽

Vol 3 (10) ◽

pp. 2067-2074 ◽

Cited By ~ 1

Author(s):

Yi-Chen Li ◽

Jyh-Horng Wang ◽

Li-Kai Tsai ◽

Yun-An Chen ◽

Ta-Chau Chang ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Precursor Cells ◽

Adipose Derived Stem Cells ◽

Marrow Mesenchymal Stem Cells

A novel compound, 3,6-bis(1-methyl-4-vinylpyridinium)-carbazole diiodide, is used as a marker for distinguishing living neural stem/precursor cells (NSPCs) from adipose-derived stem cells (ADSCs) and bone marrow mesenchymal stem cells (BMMSCs).

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Histomorphometric Evaluation of Allogeneic Transplantation of Bone Marrow Mesenchymal Stem Cells in Azoospermic Mice Model

The Indonesian Biomedical Journal ◽

10.18585/inabj.v10i2.395 ◽

2018 ◽

Vol 10 (2) ◽

pp. 171-8 ◽

Cited By ~ 1

Author(s):

Ahmad Mozafar ◽

Davood Mehrabani ◽

Akbar Vahdati ◽

Ebrahim Hosseini ◽

Mohsen Forouzanfar

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Stem Cell ◽

Cell Therapy ◽

Control Group ◽

Allogeneic Bone ◽

Control Groups ◽

Marrow Mesenchymal Stem Cells ◽

And Control

BACKGROUND: Stem cell-based therapy is one of the newest and evolving techniques in reproductive medicine. The aim of this study was to investigate the effect of allogeneic bone marrow mesenchymal stem cells (BM-MSCs) transplantation on the testis of busulfan induced azoospermia in Balb/C mice.METHODS: Eighteen adult Balb/C mice were divided into three equal groups including control, busulfan and busulfan+cell therapy (busul+CT). For induction of azoospermia, busulfan and busul+CT groups received two injections of 10 mg/Kg of busulfan intraperitoneally with 21 days interval. In the cell therapy group 35 days after the last injection of busulfan, cluster of differentiation (CD)90+/CD34-/CD45- BM-MSCs were injected into the efferent duct of testis. Eight weeks after the BM-MSCs therapy, mice were sacrificed and tissues were taken for histological and histomorphometric evaluations.RESULTS: In busul+CT group, cellular and total diameters and cellular and cross-sectional areas significantly increased in comparison to busulfan group (p˂0.001), but there were no significant differences between busul+CT and control group (p˃0.05). Numerical density and tubular count per area unit in busul+CT and control groups were significantly less than busulfan group (p˂0.001), but there were no significant difference between busul+CT and control group (p˃0.05). The luminal diameter and area showed no significant change in all groups (p˃0.05). In busul+CT group, spermatogenesis index significantly increased when compared to busulfan and control groups (p˂0.001 and p˂0.05, respectively).CONCLOSION: Histomorphometric findings showed CD90+/CD34-/CD45- BM-MSCs transplantation on the testis of busulfan-induced azoospermic in Balb/C mice recovered spermatogenesis.KEYWORDS: mesenchymal stem cell, cell therapy, azoospermia, busulfan, mouse

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