Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplification

2015 ◽  
Vol 7 (24) ◽  
pp. 10243-10250 ◽  
Author(s):  
Xing Yan ◽  
Wenkai Li ◽  
Keyi Liu ◽  
Le Deng
Keyword(s):  
Signal Amplification ◽  
Dnase I ◽  
Fluorimetric Determination ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Fluorescent Aptasensor

An elegant aptasensor was developed for dual fluorimetric determination ofSalmonella paratyphi Athrough DNase I-assisted target recycling enlargement.

Highly sensitive and selective detection of miRNA: DNase I-assisted target recycling using DNA probes protected by polydopamine nanospheres

2015 ◽  
Vol 51 (11) ◽  
pp. 2156-2158 ◽  
Author(s):  
Yi Xie ◽  
Xiaoyan Lin ◽  
Yishun Huang ◽  
Rujun Pan ◽  
Zhi Zhu ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Dnase I ◽  
Dna Probes ◽  
Selective Detection ◽  
Protective Properties ◽  
Target Recycling ◽  
Amplification Method ◽  
Highly Sensitive ◽  
Nuclease Digestion

Based on the protective properties of polydopamine nanospheres for DNA probes against nuclease digestion, we have developed a DNase I-assisted target recycling signal amplification method for highly sensitive and selective detection of miRNA.

Highly sensitive voltammetric determination of kanamycin based on aptamer sensor for signal amplification

2016 ◽  
Vol 8 (16) ◽  
pp. 3366-3372 ◽  
Author(s):  
Hai-Yan Song ◽  
Tian-Fang Kang ◽  
Na-Na Li ◽  
Li-Ping Lu ◽  
Shui-Yuan Cheng
Keyword(s):  
Horseradish Peroxidase ◽  
Signal Amplification ◽  
Voltammetric Determination ◽  
Complementary Dna ◽  
Highly Sensitive ◽  
Aptamer Sensor

In this paper, a novel aptamer sensor for kanamycin was prepared by using a complementary DNA (cDNA) strand of kanamycin aptamer as sensor and horseradish peroxidase (HRP) as a biocatalyst for signal amplification.

A QCM Immunosensor for Rapid Determination of Ractopamine in Food Based on Enzyme-AuNPs for Signal Amplification and Magnetic β-Cyclodextrins for Extraction

2015 ◽  
Vol 738-739 ◽  
pp. 56-60
Author(s):  
Shu Xian Chen ◽  
Jing Liu ◽  
Dao Dong Pan ◽  
Ning Gan
Keyword(s):  
Signal Amplification ◽  
Rapid Determination ◽  
Screening Method ◽  
High Sensitivity ◽  
The Novel ◽  
Highly Sensitive ◽  
Reliable Quantification ◽  
Highly Sensitive Detection ◽  
Amplification Strategy

An enzyme amplified immunosensor for highly sensitive detection of Ractopamine (RAC) in foodstuff was developed based on quartz crystal microbalance (QCM). The high sensitivity was achieved by enzyme-AuNPs signal amplification strategy and magnetic β-cyclodextrins (β-CD) enrichment capacity. The novel QCM immunosensor which combines with the advantages of high selectivity of immunoassays and the high sensitivity of QCM has been developed for the determination of trace residues of RAC in food production. Under optimum conditions, the differences in the frequencies (∆f) of the QCM were proportional to the concentration of RAC over the range from 0.01 to 10 ng mL-1. The minimal detection limit was 0.01 ng mL-1. Due to its high sensitivity, acceptable stability and good selectivity, the immunosensor realized reliable quantification of RAC in real foodstuff. The proposed project has the potential to become a successful on-site screening method in food safety.

Carbon nanoparticle-protected aptamers for highly sensitive and selective detection of biomolecules based on nuclease-assisted target recycling signal amplification

2014 ◽  
Vol 50 (57) ◽  
pp. 7646-7648 ◽  
Author(s):  
Xiaoyan Lin ◽  
Liang Cui ◽  
Yishun Huang ◽  
Ya Lin ◽  
Yi Xie ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Carbon Nanoparticles ◽  
Sensitive Detection ◽  
Selective Detection ◽  
Carbon Nanoparticle ◽  
Target Recycling ◽  
Amplification Method ◽  
Highly Sensitive ◽  
Detection Of Biomolecules ◽  
Highly Sensitive Detection

A nuclease-assisted target recycling signal amplification method based on carbon nanoparticles for highly sensitive detection of biomolecules was developed.

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