A QCM Immunosensor for Rapid Determination of Ractopamine in Food Based on Enzyme-AuNPs for Signal Amplification and Magnetic β-Cyclodextrins for Extraction

2015 ◽  
Vol 738-739 ◽  
pp. 56-60
Author(s):  
Shu Xian Chen ◽  
Jing Liu ◽  
Dao Dong Pan ◽  
Ning Gan
Keyword(s):  
Signal Amplification ◽  
Rapid Determination ◽  
Screening Method ◽  
High Sensitivity ◽  
The Novel ◽  
Highly Sensitive ◽  
Reliable Quantification ◽  
Highly Sensitive Detection ◽  
Amplification Strategy

An enzyme amplified immunosensor for highly sensitive detection of Ractopamine (RAC) in foodstuff was developed based on quartz crystal microbalance (QCM). The high sensitivity was achieved by enzyme-AuNPs signal amplification strategy and magnetic β-cyclodextrins (β-CD) enrichment capacity. The novel QCM immunosensor which combines with the advantages of high selectivity of immunoassays and the high sensitivity of QCM has been developed for the determination of trace residues of RAC in food production. Under optimum conditions, the differences in the frequencies (∆f) of the QCM were proportional to the concentration of RAC over the range from 0.01 to 10 ng mL-1. The minimal detection limit was 0.01 ng mL-1. Due to its high sensitivity, acceptable stability and good selectivity, the immunosensor realized reliable quantification of RAC in real foodstuff. The proposed project has the potential to become a successful on-site screening method in food safety.

Highly sensitive non-enzymatic electrochemical glucose sensor surpassing water oxidation interference

2021 ◽  
Author(s):  
Neha Thakur ◽  
Debaprasad Mandal ◽  
Tharamani Chikka Nagaiah
Keyword(s):  
Water Oxidation ◽  
Glucose Sensor ◽  
High Sensitivity ◽  
The Novel ◽  
Enzymatic Sensor ◽  
Highly Sensitive ◽  
Sensitive Determination

An electrochemical non-enzymatic sensor based on NiVP/Pi material was developed for the selective and sensitive determination of glucose. The novel sensor shows ultra-high sensitivity of 6.04 mA μM-1 cm-2 with...

scholarly journals A microchip electrophoresis-based fluorescence signal amplification strategy for highly sensitive detection of biomolecules

2017 ◽  
Vol 53 (2) ◽  
pp. 455-458 ◽  
Author(s):  
Yingfeng Qin ◽  
Liangliang Zhang ◽  
Shuting Li ◽  
Jingjin Zhao ◽  
Yong Huang ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Microchip Electrophoresis ◽  
Sensitive Detection ◽  
Fluorescence Signal ◽  
Highly Sensitive ◽  
Fluorescence Signal Amplification ◽  
Detection Of Biomolecules ◽  
Highly Sensitive Detection ◽  
Amplification Strategy

A microchip electrophoresis-based fluorescence signal amplification strategy was developed for highly sensitive detection of biomolecules.

Highly sensitive detection of nucleic acids using a cascade amplification strategy based on exonuclease III-assisted target recycling and conjugated polyelectrolytes

The Analyst ◽  
2018 ◽  
Vol 143 (18) ◽  
pp. 4267-4272 ◽  
Author(s):  
Biqing Bao ◽  
Yanrui Pan ◽  
Bingbing Gu ◽  
Jia Chen ◽  
Yu Xu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Signal Amplification ◽  
Conjugated Polyelectrolytes ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Exo Iii ◽  
Highly Sensitive Detection ◽  
Amplification Strategy ◽  
Cascade Amplification

A ratiometric and cascade amplification strategy that combines the signal amplification and effecitive FRET property of CPEs with the Exo III-assisted target recycling method has been developed for DNA detection.

An ATP-fueled nucleic acid signal amplification strategy for highly sensitive microRNA detection

2018 ◽  
Vol 54 (77) ◽  
pp. 10897-10900 ◽  
Author(s):  
Zhi-Bin Wen ◽  
Wen-Bin Liang ◽  
Ying Zhuo ◽  
Cheng-Yi Xiong ◽  
Ying-Ning Zheng ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Nucleic Acid ◽  
Signal Amplification ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Strand Displacement ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Amplification Strategy

Herein, an adenosine triphosphate (ATP)-fueled nucleic acid signal amplification strategy based on toehold-mediated strand displacement (TMSD) and fluorescence resonance energy transfer (FRET) was proposed for highly sensitive detection of microRNA-21.

Template-free multiple signal amplification for highly sensitive detection of cancer cell-derived exosomes

2021 ◽  
Author(s):  
Lei Wang ◽  
Ying Deng ◽  
Yue Huang ◽  
Juan Wei ◽  
Jiehua Ma ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Signal Amplification ◽  
High Sensitivity ◽  
Sensitive Detection ◽  
Multiple Signal ◽  
Amplification Method ◽  
Highly Sensitive ◽  
Template Free ◽  
Highly Sensitive Detection

Herein, we have designed a template-free multiple signal amplification method in this work for the detection of cancer cell-derived exosomes with high sensitivity. In this design, the combined utilization of...

An electrochemical immunosensor based on a multiple signal amplification strategy for highly sensitive detection of prostate specific antigen

2018 ◽  
Vol 10 (40) ◽  
pp. 4917-4925 ◽  
Author(s):  
Li Liu ◽  
Guanhui Zhao ◽  
Xue Dong ◽  
Xuan Li ◽  
Qin Wei ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Signal Amplification ◽  
Specific Antigen ◽  
Electrochemical Immunosensor ◽  
Sensitive Detection ◽  
Multiple Signal ◽  
Highly Sensitive ◽  
Sandwich Type ◽  
Highly Sensitive Detection ◽  
Amplification Strategy

A sandwich-type electrochemical immunosensor was developed based on a multiple signal amplification strategy.

scholarly journals Electrophoresis separation assisted G-quadruplex DNAzyme-based chemiluminescence signal amplification strategy on a microchip platform for highly sensitive detection of microRNA

2016 ◽  
Vol 52 (87) ◽  
pp. 12806-12809 ◽  
Author(s):  
Jian Li ◽  
Jingjin Zhao ◽  
Shuting Li ◽  
Liangliang Zhang ◽  
Yong Huang ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
G Quadruplex ◽  
Highly Sensitive Detection ◽  
Amplification Strategy ◽  
Electrophoresis Separation

An electrophoresis separation assisted G-quadruplex DNAzyme-based chemiluminescence signal amplification strategy on a microchip platform was developed for the detection of trace microRNA.

scholarly journals Highly sensitive detection of nitrobenzene by a series of fluorescent 2D zinc(ii) metal–organic frameworks with a flexible triangular ligand

RSC Advances ◽  
2021 ◽  
Vol 11 (39) ◽  
pp. 23975-23984
Author(s):  
Xue Yang ◽  
Yixia Ren ◽  
Hongmei Chai ◽  
Xiufang Hou ◽  
Zhixiang Wang ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Metal Organic Frameworks ◽  
High Sensitivity ◽  
Fluorescent Sensors ◽  
Sensitive Detection ◽  
Donor Ligands ◽  
Highly Sensitive ◽  
Metal Organic ◽  
Highly Sensitive Detection

Four fluorescent 2D Zn-MOFs based on a flexible triangular ligand and linear N-donor ligands are hydrothermally prepared and used to detect nitrobenzene in aqueous solution with high sensitivity, demonstrating their potential as fluorescent sensors.

A highly sensitive electrochemical microRNA-21 biosensor based on intercalating methylene blue signal amplification and a highly dispersed gold nanoparticles/graphene/polypyrrole composite

The Analyst ◽  
2021 ◽  
Vol 146 (8) ◽  
pp. 2679-2688
Author(s):  
Chammari Pothipor ◽  
Noppadol Aroonyadet ◽  
Suwussa Bamrungsap ◽  
Jaroon Jakmunee ◽  
Kontad Ounnunkad
Keyword(s):  
Methylene Blue ◽  
Gold Nanoparticles ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Highly Sensitive ◽  
Highly Dispersed ◽  
Polypyrrole Composite ◽  
Amplification Strategy ◽  
Dispersed Gold ◽  
Potential Tool

An ultrasensitive electrochemical biosensor based on a gold nanoparticles/graphene/polypyrrole composite modified electrode and a signal amplification strategy employing methylene blue is developed as a potential tool for the detection of miRNA-21.

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