Antihypertensive effects of oleuropein-enriched olive leaf extract in spontaneously hypertensive rats

The effects of chronic consumption of oleuropein-enriched (15% w/w) olive leaf extract (OLE) on blood pressure, endothelial function, and vascular oxidative and inflammatory status in spontaneously hypertensive rats (SHR) were evaluated.

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Effects of high dose olive leaf extract on haemodynamic and oxidative stress parameters in normotensive and spontaneously hypertensive rats

Journal of the Serbian Chemical Society ◽

10.2298/jsc140218030d ◽

2014 ◽

Vol 79 (9) ◽

pp. 1085-1097 ◽

Cited By ~ 5

Author(s):

Dragana Dekanski ◽

Nevena Mihailovic-Stanojevic ◽

Grujic Milanovic ◽

Djurdjica Jovovic ◽

Zoran Miloradovic

Keyword(s):

Blood Pressure ◽

Heart Rate ◽

Glutathione Reductase ◽

Spontaneously Hypertensive Rats ◽

Leaf Extract ◽

High Dose ◽

Olive Leaf ◽

Hypertensive Rats ◽

Spontaneously Hypertensive ◽

Olive Leaf Extract

Antihypertensive activity of natural antioxidant, olive leaf extract (OLE) is known, but its influence on cardiovascular system when administered in a high dose has not been investigated yet. Our aim was to determine the acute effects of excessive intake of standardized OLE on blood pressure, heart rate and oxidative status in both spontaneously hypertensive rats and normotensive Wistar rats. Systolic arterial pressure and heart rate were measured using a tail-cuff, pneumatic pulse detector, before, 60 and 120 minutes after intragastric OLE administration. Activities of catalase, glutathione peroxidase, superoxide dismutase (SOD) and glutathione reductase in erythrocytes, as well as lipid peroxidation in plasma (pTBARS) were measured at the same time points, spectrophotometrically. High-dose OLE did not influence blood pressure, heart rate and pTBARS in normotensive rats, while SOD, catalase, and glutathione reductase activities significantly increased. The same dose significantly decreased blood pressure in hypertensive rats, but increased pTBARS and SOD activity. Excessive oral intake of OLE induced moderate hypotensive effects in spontaneously hypertensive rats only, suggesting absence of harmful haemodynamic effects after oral overdose in both rats strain. However, its prooxidative role when given in high dose in hypertensive organism should not be neglected.

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Olive leaf extract attenuates adriamycin‐induced focal segmental glomerulosclerosis in spontaneously hypertensive rats via suppression of oxidative stress, hyperlipidemia, and fibrosis

Phytotherapy Research ◽

10.1002/ptr.6920 ◽

2020 ◽

Cited By ~ 1

Author(s):

Danijela Karanovic ◽

Nevena Mihailovic‐Stanojevic ◽

Zoran Miloradovic ◽

Milan Ivanov ◽

Una‐Jovana Vajic ◽

...

Keyword(s):

Oxidative Stress ◽

Focal Segmental Glomerulosclerosis ◽

Spontaneously Hypertensive Rats ◽

Leaf Extract ◽

Olive Leaf ◽

Hypertensive Rats ◽

Spontaneously Hypertensive ◽

Olive Leaf Extract ◽

Segmental Glomerulosclerosis

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Metformin Improves Endothelial Function and Reduces Blood Pressure in Diabetic Spontaneously Hypertensive Rats Independent from Glycemia Control: Comparison to Vildagliptin

Scientific Reports ◽

10.1038/s41598-017-11430-7 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 15

Author(s):

Mahdi Hamidi Shishavan ◽

Robert H. Henning ◽

Azuwerus van Buiten ◽

Maaike Goris ◽

Leo E. Deelman ◽

...

Keyword(s):

Blood Pressure ◽

Endothelial Function ◽

Spontaneously Hypertensive Rats ◽

Hypertensive Rats ◽

Spontaneously Hypertensive ◽

Control Comparison

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Abstract 261: Chloroquine, an Inhibitor of Endosomal Toll-like Receptors, Improves Blood Pressure and Endothelial Function in Spontaneously Hypertensive Rats

Hypertension ◽

10.1161/hyp.64.suppl_1.261 ◽

2014 ◽

Vol 64 (suppl_1) ◽

Author(s):

Cameron G McCarthy ◽

Camilla F Wenceslau ◽

Styliani Goulopoulou ◽

Safia Ogbi ◽

Takayuki Matsumoto ◽

...

Keyword(s):

Blood Pressure ◽

Endothelial Function ◽

Innate Immune System ◽

Spontaneously Hypertensive Rats ◽

Innate Immune ◽

Hypertensive Rats ◽

Dnase Ii ◽

Spontaneously Hypertensive ◽

Immune System Activation ◽

System Activation

HBPR 2014 Circulating mitochondrial DNA (mtDNA) is elevated in spontaneously hypertensive rats (SHR) and the enzyme responsible for its degradation, DNase II, has decreased activity in SHR tissues. Moreover, a synthetic mtDNA mimetic, specific for TLR 9, causes endothelial dysfunction and elevated blood pressure. Therefore, we sought to inhibit the contribution of mtDNA on innate immune system activation, via inhibition of Toll-like receptor (TLR)9, in SHR. We treated 12-15 week old SHR and Wistar-Kyoto rats (WKY) with chloroquine (CQ; 40mg/kg/day) or vehicle (veh; saline) for 21 days via intraperitoneal injection ( i.p. ). We hypothesized that CQ treatment would improve endothelial function and decrease blood pressure in SHR. Blood pressure was measured pre- and post-treatment via tail cuff and endothelial function was measured via mesenteric resistance artery (MRA) relaxation to acetylcholine (ACh; 10 -9 -10 -5 M) using a wire myograph. Treatment with CQ lowered post-systolic blood pressure in SHR (mmHg, Veh: 201±2 vs. CQ: 185±5 mmHg; p<0.05). No effects of treatment were observed on blood pressure in WKY (p>0.05). CQ abolished the contractile effect to high concentrations of ACh [AUC (%NE pre-contraction), Veh: 160±25 vs. CQ: 277±20; p<0.05] in MRA from SHR. However, no differences were observed in endothelium-independent relaxation to NO-donor sodium nitroprusside [E max (%NE pre-contraction), Veh: 97±12 vs. CQ: 102±26; p<0.05] in MRAs from SHR. Again, no effects of treatment were observed for endothelium-dependent or -independent relaxation in WKY (p>0.05). These data demonstrate the inhibition of endosomal TLRs, including TLR9, improves blood pressure and endothelial function in SHR. Inhibition of TLR9 abrogates the potentially deleterious contribution of increased mtDNA and impaired DNase II activity on innate immune system activation in hypertension.

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