A label free fluorescent assay for uracil-DNA glycosylase activity based on the signal amplification of exonuclease I

RSC Advances ◽  
2015 ◽  
Vol 5 (98) ◽  
pp. 80871-80874 ◽  
Author(s):  
Yefei Ma ◽  
Jingjin Zhao ◽  
Xuejun Li ◽  
Liangliang Zhang ◽  
Shulin Zhao
Keyword(s):  
Signal Amplification ◽  
Dna Glycosylase ◽  
Label Free ◽  
Fluorescent Assay ◽  
Uracil Dna Glycosylase ◽  
Exonuclease I

A label free fluorescent assay for uracil-DNA glycosylase activity was developed based on the signal amplification of exonuclease I.

A novel fluorescent assay for uracil DNA glycosylase activity built on the 3′–5′ exonuclease activity-based endonuclease IV cyclic signal amplification strategy

2020 ◽  
Vol 44 (48) ◽  
pp. 21211-21217
Author(s):  
Jing-Xuan Tian ◽  
Yan-Zhao Fang ◽  
Yi-Xuan Yang ◽  
Shuang Wu ◽  
Qiang Xiao ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Dna Glycosylase ◽  
Exonuclease Activity ◽  
Sensitive Assay ◽  
Fluorescent Assay ◽  
Uracil Dna Glycosylase ◽  
Highly Sensitive ◽  
Dna Strands ◽  
Amplification Strategy

The unique 3′-5′ exonuclease activity of endonuclease IV to DNA strands has been demonstrated, which enables the development of a novel highly sensitive assay for UDG activity.

Label-free and high-throughput bioluminescence detection of uracil-DNA glycosylase in cancer cells through tricyclic cascade signal amplification

2018 ◽  
Vol 54 (51) ◽  
pp. 6991-6994 ◽  
Author(s):  
Yan Zhang ◽  
Qing-nan Li ◽  
Chen-chen Li ◽  
Chun-yang Zhang
Keyword(s):  
Cancer Cells ◽  
High Throughput ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Dna Glycosylase ◽  
Label Free ◽  
Uracil Dna Glycosylase ◽  
Bioluminescence Method

We develop a label-free and high-throughput bioluminescence method for the sensitive detection of uracil DNA glycosylase through tricyclic cascade signal amplification.

Simple label-free and sensitive fluorescence determination of human 8-oxoG DNA glycosylase 1 activity and inhibitionviaTdT-assisted sequence extension amplification

2019 ◽  
Vol 43 (21) ◽  
pp. 8260-8265
Author(s):  
Sujing Wang ◽  
Daxiu Li ◽  
Ruo Yuan ◽  
Yun Xiang
Keyword(s):  
Signal Amplification ◽  
Sensitive Detection ◽  
Dna Glycosylase ◽  
Label Free ◽  
Fluorescence Determination

Label-free and sensitive detection of hOGG1 activity and inhibitionviaTdT-assisted sequence extension signal amplification.

A label-free and sensitive fluorescent method for the detection of uracil-DNA glycosylase activity

2015 ◽  
Vol 51 (5) ◽  
pp. 929-932 ◽  
Author(s):  
Jing Tao ◽  
Panshu Song ◽  
Yusuke Sato ◽  
Seiichi Nishizawa ◽  
Norio Teramae ◽  
...  
Keyword(s):  
Sensitive Detection ◽  
Dna Glycosylase ◽  
Label Free ◽  
Uracil Dna Glycosylase ◽  
Fluorescent Method

A label-free fluorescent method has been developed for sensitive detection of uracil-DNA glycosylase activity as well as UDG inhibitors.

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