pH-Sensitive perylene tetra-(alkoxycarbonyl) probes for live cell imaging

2016 ◽  
Vol 40 (8) ◽  
pp. 6615-6622 ◽  
Author(s):  
Yongshan Ma ◽  
Jiaofu Li ◽  
Shuguo Hou ◽  
Jinfeng Zhang ◽  
Zhiqiang Shi ◽  
...  

A novel perylene pH probe for imaging of living cells in neutral to weak basic pH changes.

2018 ◽  
Vol 42 (21) ◽  
pp. 17351-17358 ◽  
Author(s):  
Anup Kumar Bhanja ◽  
Snehasis Mishra ◽  
Ketaki Kar ◽  
Kaushik Naskar ◽  
Suvendu Maity ◽  
...  

An allyl-rhodamine Schiff base shows excellent palladium sensitivity (LOD, 95 nM) irrespective of Pd(0,ii,iv) and practical applicability is judged in living cells of RAW 264.7 (macrophage) cells.


2018 ◽  
Vol 59 (27) ◽  
pp. 2671-2678 ◽  
Author(s):  
Shengxin Chen ◽  
Rongmao Qiu ◽  
Qiuhan Yu ◽  
Xueyan zhang ◽  
Ming Wei ◽  
...  

2016 ◽  
Vol 52 (60) ◽  
pp. 9442-9445 ◽  
Author(s):  
Andrew V. Anzalone ◽  
Zhixing Chen ◽  
Virginia W. Cornish

A new cell-permeable caged oxazine fluorophore was synthesized for protein specific labeling and photoactivation in living cells.


mSphere ◽  
2016 ◽  
Vol 1 (4) ◽  
Author(s):  
H. M. van der Schaar ◽  
C. E. Melia ◽  
J. A. C. van Bruggen ◽  
J. R. P. M. Strating ◽  
M. E. D. van Geenen ◽  
...  

ABSTRACT Enteroviruses induce the formation of membranous structures (replication organelles [ROs]) with a unique protein and lipid composition specialized for genome replication. Electron microscopy has revealed the morphology of enterovirus ROs, and immunofluorescence studies have been conducted to investigate their origin and formation. Yet, immunofluorescence analysis of fixed cells results in a rather static view of RO formation, and the results may be compromised by immunolabeling artifacts. While live-cell imaging of ROs would be preferred, enteroviruses encoding a membrane-anchored viral protein fused to a large fluorescent reporter have thus far not been described. Here, we tackled this constraint by introducing a small tag from a split-GFP system into an RO-resident enterovirus protein. This new tool bridges a methodological gap by circumventing the need for immunolabeling fixed cells and allows the study of the dynamics and formation of enterovirus ROs in living cells. Like all other positive-strand RNA viruses, enteroviruses generate new organelles (replication organelles [ROs]) with a unique protein and lipid composition on which they multiply their viral genome. Suitable tools for live-cell imaging of enterovirus ROs are currently unavailable, as recombinant enteroviruses that carry genes that encode RO-anchored viral proteins tagged with fluorescent reporters have not been reported thus far. To overcome this limitation, we used a split green fluorescent protein (split-GFP) system, comprising a large fragment [strands 1 to 10; GFP(S1-10)] and a small fragment [strand 11; GFP(S11)] of only 16 residues. The GFP(S11) (GFP with S11 fragment) fragment was inserted into the 3A protein of the enterovirus coxsackievirus B3 (CVB3), while the large fragment was supplied by transient or stable expression in cells. The introduction of GFP(S11) did not affect the known functions of 3A when expressed in isolation. Using correlative light electron microscopy (CLEM), we showed that GFP fluorescence was detected at ROs, whose morphologies are essentially identical to those previously observed for wild-type CVB3, indicating that GFP(S11)-tagged 3A proteins assemble with GFP(S1-10) to form GFP for illumination of bona fide ROs. It is well established that enterovirus infection leads to Golgi disintegration. Through live-cell imaging of infected cells expressing an mCherry-tagged Golgi marker, we monitored RO development and revealed the dynamics of Golgi disassembly in real time. Having demonstrated the suitability of this virus for imaging ROs, we constructed a CVB3 encoding GFP(S1-10) and GFP(S11)-tagged 3A to bypass the need to express GFP(S1-10) prior to infection. These tools will have multiple applications in future studies on the origin, location, and function of enterovirus ROs. IMPORTANCE Enteroviruses induce the formation of membranous structures (replication organelles [ROs]) with a unique protein and lipid composition specialized for genome replication. Electron microscopy has revealed the morphology of enterovirus ROs, and immunofluorescence studies have been conducted to investigate their origin and formation. Yet, immunofluorescence analysis of fixed cells results in a rather static view of RO formation, and the results may be compromised by immunolabeling artifacts. While live-cell imaging of ROs would be preferred, enteroviruses encoding a membrane-anchored viral protein fused to a large fluorescent reporter have thus far not been described. Here, we tackled this constraint by introducing a small tag from a split-GFP system into an RO-resident enterovirus protein. This new tool bridges a methodological gap by circumventing the need for immunolabeling fixed cells and allows the study of the dynamics and formation of enterovirus ROs in living cells.


2011 ◽  
Vol 21 (35) ◽  
pp. 13470 ◽  
Author(s):  
Panshu Song ◽  
Xiaotong Chen ◽  
Yu Xiang ◽  
Lei Huang ◽  
Zhaojuan Zhou ◽  
...  

RSC Advances ◽  
2015 ◽  
Vol 5 (21) ◽  
pp. 15778-15783 ◽  
Author(s):  
Mengqiang Liu ◽  
Minshan Hu ◽  
Qian Jiang ◽  
Zhiyun Lu ◽  
Yan Huang ◽  
...  

A novel coumarin derivative was synthesized and its application in live cell imaging was demonstrated.


2017 ◽  
Vol 46 (28) ◽  
pp. 9245-9252 ◽  
Author(s):  
Anup Kumar Bhanja ◽  
Snehasis Mishra ◽  
Krishna Das Saha ◽  
Chittaranjan Sinha

Allylether-Rhodamine selectively invites Pd2+from mixture of other cations and Pd(0) in H2O-MeCN medium and exhibits maroon emission due to C(allyl)-O bond cleavage and opening of spirolactam ring. The LOD is 50 nM at pH 7. Imaging Pd2+species in living cells under physiological conditions is achieved.


2017 ◽  
Vol 15 (18) ◽  
pp. 3947-3954 ◽  
Author(s):  
Chang Liu ◽  
Xiaojie Jiao ◽  
Song He ◽  
Liancheng Zhao ◽  
Xianshun Zeng

A bifluorophore-based ratiometric fluorescent probe has been developed. The probe is cell-permeable and can be used as a FRET-based ratiometric fluorescent probe for lysosomal Cu2+imaging in living cells.


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