scholarly journals Influence of washing and quenching in profiling the metabolome of adherent mammalian cells: a case study with the metastatic breast cancer cell line MDA-MB-231

The Analyst ◽  
2017 ◽  
Vol 142 (11) ◽  
pp. 2038-2049 ◽  
Author(s):  
Rahul Vijay Kapoore ◽  
Rachael Coyle ◽  
Carolyn A. Staton ◽  
Nicola J. Brown ◽  
Seetharaman Vaidyanathan
Keyword(s):  
Breast Cancer ◽  
Metastatic Breast Cancer ◽  
Cell Line ◽  
Breast Cancer Cell Line ◽  
Mammalian Cells ◽  
Metastatic Breast ◽  
Cancer Cell Line ◽  
Metabolite Leakage ◽  
Adherent Mammalian Cells

1 step of PBS wash followed by quenching with 60% methanol supplemented with 70 mM HEPES results in minimal metabolite leakage.

3,6-dichloro-1,2,4,5-Tetrazine Assayed at High Doses in the Metastatic Breast Cancer Cell Line MDA-MB-231 Reduces Cell Numbers and Induces Apoptosis

2020 ◽  
Vol 16 (4) ◽  
pp. 546-550 ◽  
Author(s):  
Mohamed Abdelkarim ◽  
Khaoula Ben Younes ◽  
Ines Limam ◽  
Refka Guermazi ◽  
Amel Ben Ammaar ElGaaied ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Metastatic Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Metastatic Breast ◽  
Cancer Cell Line ◽  
Metastatic Breast Cancer Cell

Background: The lack of treatment options for patients with chemotherapy-resistant cancers is pushing the field toward the development of new therapies. 1,2,4,5-tetrazine derivatives are a class of heterocyclic compounds that exhibit a broad spectrum of antitumor activities. Objective: The purpose of this study was to assess the biological activity of four s-tetrazine derivatives by substitution of two chloride atom of 3,6-dichloro-1,2,4,5-tetrazine with long hydrophobic side chains. Methods: We analyzed the anti-proliferative effects of four s-tetrazine derivatives with MTT assay and their pro-apoptotic effect with AV/ IP flow cytometry analysis and Hoechst 33342 staining. Results: We demonstrated that 3,6-dichloro-1,2,4,5-tetrazine (compound (1)) has a cytotoxic effect and induces apoptosis. Conclusion: 3,6-dichloro-1,2,4,5-tetrazine presents a new cytotoxic drug against metastatic breast cancer cell line MDA-MB-231 in vitro.

scholarly journals Cytotoxic and pro-apoptotic effects of Abrus precatorius L. on human metastatic breast cancer cell line, MDA-MB-231

2012 ◽  
Vol 65 (3) ◽  
pp. 407-417 ◽  
Author(s):  
Mohammed Shafi Sofi ◽  
M. K. Sateesh ◽  
Mohsin Bashir ◽  
G. Harish ◽  
T. R. Lakshmeesha ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Metastatic Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Metastatic Breast ◽  
Cancer Cell Line ◽  
Abrus Precatorius ◽  
Apoptotic Effects

Evaluation of STAT3 decoy oligodeoxynucleotides' synergistic effects on radiation and/or chemotherapy in metastatic breast cancer cell line

2020 ◽  
Vol 44 (12) ◽  
pp. 2499-2511
Author(s):  
Behrooz Johari ◽  
Mohammad Rahmati ◽  
Leila Nasehi ◽  
Yousef Mortazavi ◽  
Mohammad Hasan Faghfoori ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Metastatic Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Synergistic Effects ◽  
Metastatic Breast ◽  
Cancer Cell Line ◽  
Stat3 Decoy

scholarly journals Comparative proteomic analysis implicates eEF2 as a novel target of PI3Kγ in the MDA-MB-231 metastatic breast cancer cell line

2013 ◽  
Vol 11 (1) ◽  
pp. 4 ◽  
Author(s):  
Meizhi Niu ◽  
Manuela Klingler-Hoffmann ◽  
Julie A Brazzatti ◽  
Briony Forbes ◽  
Chareeporn Akekawatchai ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Metastatic Breast Cancer ◽  
Cell Line ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Proteomic Analysis ◽  
Metastatic Breast ◽  
Cancer Cell Line ◽  
Comparative Proteomic Analysis ◽  
Novel Target

scholarly journals Overexpression of Metastatic Related MicroRNAs, Mir-335 and Mir-10b, by Staphylococcal Enterotoxin B in the Metastatic Breast Cancer Cell Line

2015 ◽  
Vol 5 (2) ◽  
pp. 255-259 ◽  
Author(s):  
Mohammad Foad Heidary ◽  
Hamideh Mahmoodzadeh Hosseini ◽  
Elnaz Mehdizadeh Aghdam ◽  
Mohammad Reza Nourani ◽  
Reza Ranjbar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Metastatic Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Metastatic Breast ◽  
Cancer Cell Line ◽  
Staphylococcal Enterotoxin B ◽  
Enterotoxin B
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