scholarly journals A binary matrix for the rapid detection and characterization of small-molecule cardiovascular drugs by MALDI-MS and MS/MS

2018 ◽  
Vol 10 (6) ◽  
pp. 572-578 ◽  
Author(s):  
Jinlan Dong ◽  
Wenjing Ning ◽  
Daniel J. Mans ◽  
Jamie D. Mans
Keyword(s):  
Small Molecule ◽  
Hydroxycinnamic Acid ◽  
Cardiovascular Drugs ◽  
Maldi Ms ◽  
Negative Ion ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Binary Matrix ◽  
Negative Ion Mode

A mixture of α-cyano-4-hydroxycinnamic acid and 1,5-diaminonaphthalene was discovered as a novel binary matrix for the qualitative analysis of 14 small-molecule (∼250–550 Da) cardiovascular drugs by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and MS/MS in either positive or negative ion mode.

265 MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY (MALDI-MS) CHARACTERIZATION OF SPERM LIPID PROFILES OF BULLS WITH DIFFERENT CAPACITIES OF EMBRYO IN VITRO PRODUCTION

2010 ◽  
Vol 22 (1) ◽  
pp. 289
Author(s):  
C. R Ferreira ◽  
J. C. Borges ◽  
L. F. A. Santos ◽  
F. C. Gozzo ◽  
P. H. Franscechini ◽  
...  
Keyword(s):  
Laser Desorption ◽  
Lipid Profiles ◽  
Maldi Ms ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
In Vitro Production ◽  
Vitro Production ◽  
Matrix Assisted Laser

Matrix-assisted laser desorption-ionization mass spectrometry (MALDI-MS) has been applied to study sperm lipid profiles. Lipids are known to play a crucial role in sperm membrane physico-chemical behavior during cryopreservation. In this work, we show the results of characterization of sperm lipid profiles from 2 bulls with different capacities of in vitro embryo production by MALDI-MS direct analysis. The bull capacities judged by the rate of blastocyst formation after IVP with semen from seven different ejaculates per each animal were 19.1 and 35.3% for bulls 1 and 2, respectively (P < 0.05). For MALDI-MS analysis, frozen semen from each ejaculate was thawed in water at 25°C for 40 s. Sperm was washed 3 times by centrifugatin in 1 mL of PBS at 3000 × g for 10 min. Samples were stored at -20°C in 200 μL of methanol:PBS (vol/vol) solution until analysis. A Synapt HDMS mass spectrometer (Waters Corp., Milford, MA, USA) equipped with a MALDI was used. All spectra were collected for 45 s in the positive ion mode at the mass range of m/z 450 to 1200. The volume of 1 μL of the semen pellet was spotted in the target plate and allowed to dry. Afterward, 1 μL of 2,5-dihydroxybenzoic acid (DHB) was added as matrix. The 50 most intense monoisotopic ions were considered for principal component analysis (PCA). Values of m/z and relative ion intensities were processed using the software Pirouette v.3.11 (Infometrix, Woodinville, WA, USA). Direct MALDI-MS analysis of bulls 1 and 2 spermatozoa with no extraction provided informative spectra containing either [M + Na]+ or [M + H]+ ions characteristic of sphingomyelins, such as m/z 753.6 for SM 18:0, phosphocholines (m/z 780.6 for PC 34 : 2; 782.6 for PC 34 : 1; 806.6 for PC 36 : 6; 808.6 for 36 :2; 828.6 for PC38 : 6; and 830.6 for PC38 : 5), plasmalogens (m/z 790.6 for 1-palmitenyl-2-docosahexanoyl-GPC and 814.6 for 1-palmityl-2-docosaheaenoyl-GPC); and triacylglycerols (m/z 881.7 for sn-glycerol-palmitoleate-oleate-oleate). PCA showed clear separation between bulls 1 and 2 ejaculates, indicating that each bull presented a characteristic and reproducible (from different ejaculates) profiles. Differences in the relative intensities of the ions mentioned above contributed for bulls 1 and 2 differentiation by PCA. PC1 and PC2 explained 86.5% of the data variance. In conclusion, a fast sample preparation protocol followed by MALDI-MS appears to provide characteristic lipid fingerprints for crude spermatozoa (ejaculates) of bulls with different capacities of embryo in vitro production. Experiments involving a larger and more statically relevant set of samples are underway. We thank the Brazilian research foundations FAPESP (2008/10756-7) and CNPq.

1,5-Diaminonaphthalene functionalized carbon nanodots as a novel matrix for the analysis of small molecules by matrix-assisted laser desorption/ionization mass spectrometry

2019 ◽  
Vol 11 (8) ◽  
pp. 1131-1136 ◽  
Author(s):  
Yueying Zhang ◽  
Dan Gao ◽  
Shangfu Li ◽  
Weili Wei ◽  
Jinshun Lin ◽  
...  
Keyword(s):  
Small Molecules ◽  
Direct Detection ◽  
Laser Desorption ◽  
Negative Ion ◽  
Carbon Nanodots ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Negative Ion Mode ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

1,5-Diaminonaphthalene functionalized carbon nanodots (DAN-CDs) were explored as a matrix for direct detection of small molecules by MALDI-MS in negative-ion mode.

scholarly journals Laser Desorption/Ionization Mass Spectrometry as a Potential Tool for Evaluation of Hydroxylation Degree of Various Types of Titanium Dioxide Materials

Materials ◽  
2021 ◽  
Vol 14 (22) ◽  
pp. 6848
Author(s):  
Małgorzata Kasperkowiak ◽  
Monika Kurowska ◽  
Maciej Zalas ◽  
Rafał Frański
Keyword(s):  
Mass Spectrometry ◽  
Surface Characterization ◽  
Laser Desorption ◽  
Negative Ion ◽  
Ionization Mass Spectrometry ◽  
Hydroxyl Group ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Negative Ion Mode

For many applications, TiO2 must have a unique surface structure responsible for its desirable physicochemical properties. Therefore the fast and easy methods of TiO2 surface characterization are of great interest. Heated TiO2 samples and dye-modified TiO2 samples were analyzed by laser desorption/ionization mass spectrometry. In the negative ion mode, two types of ions were detected, namely (TiO2)n− and (TiO2)nOH−. It has been established that the samples can be differentiated based on the relative ion abundances, especially with respect to the free hydroxyl group population. It indicates that laser desorption ionization mass spectrometry has the potential for the investigation of the surface properties of various TiO2 materials.

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