First HPLC method for the simultaneous quantification of levetiracetam, zonisamide, lamotrigine, pentylenetetrazole and pilocarpine in rat plasma and brain

2018 ◽  
Vol 10 (5) ◽  
pp. 515-525 ◽  
Author(s):  
Beatriz M. Fonseca ◽  
Márcio Rodrigues ◽  
Gilberto Alves
Keyword(s):  
Simultaneous Determination ◽  
Antiepileptic Drugs ◽  
Rat Plasma ◽  
Hplc Method ◽  
Simultaneous Quantification ◽  
First Time

A new HPLC-DAD method is herein reported for the first time for the simultaneous determination of three antiepileptic drugs [levetiracetam (LEV), zonisamide (ZNS) and lamotrigine (LTG)] and two convulsant agents [pentylenetetrazole (PTZ) and pilocarpine (PIL)] in rat plasma and brain matrices.

scholarly journals Simultaneous Determination and Pharmacokinetic Comparisons of Multi-Ingredients after Oral Administration ofRadix Salviae MiltiorrhizaeExtract, Hawthorn Extract, and a Combination of Both Extracts to Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Yu-Qiang Liu ◽  
Qian Cai ◽  
Chang Liu ◽  
Feng-Wei Bao ◽  
Zhen-Qiu Zhang
Keyword(s):  
Oral Administration ◽  
Simultaneous Determination ◽  
Pharmacokinetic Study ◽  
Rosmarinic Acid ◽  
Rat Plasma ◽  
Hplc Method ◽  
Radix Salviae Miltiorrhizae ◽  
Salviae Miltiorrhizae ◽  
Hawthorn Extract

A simple and sensitive HPLC method was developed for simultaneous determination of danshensu (DSS), rosmarinic acid (RA), lithospermic acid (LA), salvianolic acid B (SAB), and hyperoside (HP) in rat plasma. This method validated was successfully applied to the pharmacokinetic study of the main active ingredients after oral administration ofRadix Salviae Miltiorrhizaeextract (SME), hawthorn extract (HTE), and a combination of both extracts (2.5 : 1) to rats. The results indicated that there have been great differences in pharmacokinetics between a single extract and a combination of both extracts. A combination of both extracts can enhance their bioavailabilities and delay the elimination of SAB and DSS in rats.

scholarly journals Simultaneous Determination of Four Major Constituents of Semen Vaccariae Using HPLC-DAD

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Haijiang Zhang ◽  
Wei Yao ◽  
Yunyun Chen ◽  
Peipei He ◽  
Yao Chen ◽  
...  
Keyword(s):  
Quality Control ◽  
Quantitative Analysis ◽  
Simultaneous Determination ◽  
Chromatographic Separation ◽  
Gradient Elution ◽  
Hplc Method ◽  
Frying Temperature ◽  
Simultaneous Quantification ◽  
Frying Process

A simple and reliable HPLC method was developed and validated for the simultaneous quantification of four major constituents in Semen Vaccariae. The chromatographic separation was performed on an Agilent Zorbax SB-C18 column with gradient elution using methanol and water. The calibration curves showed good linearity of R2 > 0.9999 with LOQs (S/N = 10) of 0.20–1.16 μg/mL. The precision was evaluated by intra- and inter-day assays and R.S.D. values were less than 2.09%. The recovery rates were between 97.0% and 105.0%. The developed method was applied to the quantitative analysis of Semen Vaccariae and its stir-fried products. During the stir-frying process, vaccarin degraded and yielded isovitexin-2″- O-arabinoside. The preferable stir-frying temperature is around 120°C. The developed HPLC method can be applied to the quality control of crude and stir-fried Semen Vaccariae.

scholarly journals HPLC method for the simultaneous determination of four compounds in rat plasma after intravenous administration of Portulaca oleracea L. extract

2012 ◽  
Vol 48 (1) ◽  
pp. 163-170 ◽  
Author(s):  
Zhongzhe Cheng ◽  
Ming Xie ◽  
Wenjie Zhang ◽  
Lan Cheng ◽  
Yang Du ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Intravenous Administration ◽  
Internal Standard ◽  
Sample Pretreatment ◽  
Rat Plasma ◽  
Hplc Method ◽  
Single Step ◽  
Portulaca Oleracea ◽  
Coumaric Acid

The objective of the present study was to develop a simple and selective HPLC method for the simultaneous determination of hesperidin (HP), caffeic acid (CA), ferulic acid (FA) and p-coumaric acid (p-CA) in rat plasma after intravenous administration of Portulaca oleracea L. extract (POE). With the hyperoside as the internal standard, the sample pretreatment procedure involved simple single-step extraction with methanol of 0.2 mL plasma. The mobile phase consisted of methanol-acetonitrile-tetrahydrofuran-0.5% glacial acetic acid (5:3:18:74, v/v/v/v). The calibration curves were linear over the range of 0.1-25 µg mL-1, 0.1-25 µg mL-1, 0.1-25 µg mL-1and 0.015-3 µg mL-1 for HP, CA, FA and p-CA, respectively. The method developed was suitable for the pharmacokinetic study of HP, CA, FA and p-CA in rats after intravenous administration of POE.

scholarly journals Method validation for simultaneous quantification of whey proteins in dietary supplements by HPLC

2019 ◽  
Vol 2 (1) ◽  
pp. 32-38
Author(s):  
Hong Ngoc Nguyen Thi ◽  
Thanh Hoa Mac Thi ◽  
Thanh An Vu Thi ◽  
Thanh Ha Pham Thi ◽  
Khanh Cao Cong ◽  
...  
Keyword(s):  
Dietary Supplements ◽  
Simultaneous Determination ◽  
Method Validation ◽  
Mobile Phase ◽  
Whey Proteins ◽  
Hplc Method ◽  
Simultaneous Quantification ◽  
Precision And Accuracy ◽  
Beta Lactoglobulin

A HPLC method has been validated for simultaneous determination of Alpha-lactalbumin (α- LA) and Beta- lactoglobulin (β-LG) contents in dietary supplements by HPLC-PDA. The method was carried out in C18 column with a gradient of 0.1% TFA/ water – 0.1% TFA/ acetonitrile as mobile phase. The proteins were detected at 215 nm wavelength within 50 minutes. The method was validated in specificity, linearity, precision and accuracy; and then was applied to analyze several random dietary supplements.

scholarly journals Development of RP-HPLC method for simultaneous determination of docetaxel and curcumin in rat plasma: Validation and stability

2017 ◽  
Vol 12 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Dong Wuk Kim ◽  
Abid Mehmood Yousaf ◽  
Dong Xun Li ◽  
Jong Oh Kim ◽  
Chul Soon Yong ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Rat Plasma ◽  
Hplc Method ◽  
Rp Hplc
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