Method validation for simultaneous quantification of whey proteins in dietary supplements by HPLC
Keyword(s):
A HPLC method has been validated for simultaneous determination of Alpha-lactalbumin (α- LA) and Beta- lactoglobulin (β-LG) contents in dietary supplements by HPLC-PDA. The method was carried out in C18 column with a gradient of 0.1% TFA/ water – 0.1% TFA/ acetonitrile as mobile phase. The proteins were detected at 215 nm wavelength within 50 minutes. The method was validated in specificity, linearity, precision and accuracy; and then was applied to analyze several random dietary supplements.
2013 ◽
Vol 781-784
◽
pp. 787-791
◽
Keyword(s):
2011 ◽
Vol 94
(1)
◽
pp. 106-109
◽
2012 ◽
Vol 7
(9)
◽
pp. 1934578X1200700
◽
2016 ◽
Vol 2016
◽
pp. 1-8
◽
Keyword(s):
2020 ◽
Vol 10
(2)
◽
pp. 49-55
Keyword(s):