Micropassage-embedding composite hydrogel fibers enable quantitative evaluation of cancer cell invasion under 3D coculture conditions

Lab on a Chip ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 1378-1387 ◽  
Author(s):  
Manami Sugimoto ◽  
Yoichi Kitagawa ◽  
Masumi Yamada ◽  
Yuya Yajima ◽  
Rie Utoh ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Quantitative Evaluation ◽  
Cell Invasion ◽  
Three Dimensional ◽  
Composite Hydrogel ◽  
Cancer Cell Invasion ◽  
New System

A new system for quantitatively evaluating cancer cell invasion in a three-dimensional environment was developed using composite hydrogel microfibers having a micropassage.

Microfluidic Device Fabricated by Three-Dimensional Lithography for Observation of Cancer Cell Invasion Process

2018 ◽  
Vol 138 (9) ◽  
pp. 435-440
Author(s):  
Saeko Tachikawa ◽  
Shohei Kaneda ◽  
Momoko Kumemura ◽  
Rui Sato ◽  
Takuya Tsukamoto ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Microfluidic Device ◽  
Cell Invasion ◽  
Three Dimensional ◽  
Invasion Process ◽  
Cancer Cell Invasion

scholarly journals Breast Cancer Cell Invasion into a Three Dimensional Tumor-Stroma Microenvironment

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Danh Truong ◽  
Julieann Puleo ◽  
Alison Llave ◽  
Ghassan Mouneimne ◽  
Roger D. Kamm ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cell Invasion ◽  
Three Dimensional ◽  
Tumor Stroma ◽  
Cancer Cell Invasion ◽  
Breast Cancer Cell Invasion

scholarly journals Collective cancer cell invasion requires RNA accumulation at the invasive front

2020 ◽  
Vol 117 (44) ◽  
pp. 27423-27434 ◽  
Author(s):  
George Chrisafis ◽  
Tianhong Wang ◽  
Konstadinos Moissoglu ◽  
Alexander N. Gasparski ◽  
Yeap Ng ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Cancer Progression ◽  
Cell Invasion ◽  
Single Cells ◽  
Three Dimensional ◽  
Nucleotide Exchange ◽  
Cancer Cell Invasion ◽  
Invasive Front ◽  
Rna Accumulation

Localization of RNAs at protrusive regions of cells is important for single-cell migration on two-dimensional surfaces. Protrusion-enriched RNAs encode factors linked to cancer progression, such as the RAB13 GTPase and the NET1 guanine nucleotide exchange factor, and are regulated by the tumor-suppressor protein APC. However, tumor cells in vivo often do not move as single cells but rather utilize collective modes of invasion and dissemination. Here, we developed an inducible system of three-dimensional (3D) collective invasion to study the behavior and importance of protrusion-enriched RNAs. We find that, strikingly, both theRAB13andNET1RNAs are enriched specifically at the invasive front of leader cells in invasive cell strands. This localization requires microtubules and coincides with sites of high laminin concentration. Indeed, laminin association and integrin engagement are required for RNA accumulation at the invasive front. Importantly, perturbing RNA accumulation reduces collective 3D invasion. Examination of in vivo tumors reveals a similar localization of theRAB13andNET1RNAs at potential invasive sites, suggesting that this mechanism could provide a targeting opportunity for interfering with collective cancer cell invasion.

scholarly journals Delineating the role of membrane blebs in a hybrid mode of cancer cell invasion in three-dimensional environments

2020 ◽  
Vol 133 (8) ◽  
pp. jcs236778 ◽  
Author(s):  
Asja Guzman ◽  
Rachel C. Avard ◽  
Alexander J. Devanny ◽  
Oh Sang Kweon ◽  
Laura J. Kaufman
Keyword(s):  
Cancer Cell ◽  
Cell Invasion ◽  
Three Dimensional ◽  
Cancer Cell Invasion ◽  
Hybrid Mode ◽  
Membrane Blebs
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