Polydiacetylene liposomes with phenylboronic acid tags: a fluorescence turn-on sensor for sialic acid detection and cell-surface glycan imaging

A new polydiacetylene (PDA) liposome-based sensor system was developed as a fluorescence turn-on sensor for the detection of free sialic acid (SA) in aqueous solution and in situ imaging of glycans on living cell surfaces.

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Exo-enzymatic addition of diazirine-modified sialic acid to cell surfaces enables photocrosslinking of glycoproteins

10.1101/2021.10.07.463072 ◽

2021 ◽

Author(s):

Nageswari Yarravarapu ◽

Rohit Sai Reddy Konada ◽

Narek Darabedian ◽

Nichole J. Pedowtiz ◽

Soumya N. Krishnamurthy ◽

...

Keyword(s):

Sialic Acid ◽

Cell Surface ◽

Cell Types ◽

Cell Surfaces ◽

Binding Interactions ◽

Multiple Cell ◽

Labeling Method ◽

Cell Surface Glycoconjugates

Glycan binding often mediates extracellular macromolecular recognition events. Accurate characterization of these binding interactions can be difficult because of dissociation and scrambling that occur during purification and analysis steps. Use of photocrosslinking methods has been pursued to covalently capture glycan-dependent interactions in situ however use of metabolic glycan engineering methods to incorporate photocrosslinking sugar analogs is limited to certain cell types. Here we report an exo-enzymatic labeling method to add a diazirine-modified sialic acid (SiaDAz) to cell surface glycoconjugates. The method involves chemoenzymatic synthesis of diazirine-modified CMP-sialic acid (CMP-SiaDAz), followed by sialyltransferase-catalyzed addition of SiaDAz to desialylated cell surfaces. Cell surface SiaDAz-ylation is compatible with multiple cell types and is facilitated by endogenous extracellular sialyltransferase activity present in Daudi B cells. This method for extracellular addition of α2-6-linked SiaDAz enables UV-induced crosslinking of CD22, demonstrating the utility for covalent capture of glycan-mediated binding interactions.

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Fluorescence imaging for in situ detection of cell surface sialic acid by competitive binding of 3-(dansylamino)phenylboronic acid

Analytica Chimica Acta ◽

10.1016/j.aca.2015.08.054 ◽

2015 ◽

Vol 894 ◽

pp. 85-90 ◽

Cited By ~ 11

Author(s):

Ruocan Qian ◽

Lin Ding ◽

Liwen Yan ◽

Huangxian Ju

Keyword(s):

Sialic Acid ◽

Cell Surface ◽

Fluorescence Imaging ◽

Phenylboronic Acid ◽

Competitive Binding ◽

In Situ Detection

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A Naphthalimide-Based Fluorescence “Turn-On” Probe for the Detection of Pb2+in Aqueous Solution and Living Cells

Chemistry - An Asian Journal ◽

10.1002/asia.201402946 ◽

2014 ◽

Vol 9 (12) ◽

pp. 3397-3402 ◽

Cited By ~ 34

Author(s):

Hio-Ieng Un ◽

Chang-Bo Huang ◽

Junhai Huang ◽

Chusen Huang ◽

Ti Jia ◽

...

Keyword(s):

Aqueous Solution ◽

Living Cells ◽

Turn On ◽

Fluorescence Turn On

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A highly selective and sensitive fluorescence “turn-on” probe for Ag+ in aqueous solution and live cells

Dalton Transactions ◽

10.1039/c2dt30404j ◽

2012 ◽

Vol 41 (24) ◽

pp. 7212 ◽

Cited By ~ 72

Author(s):

Lin Xu ◽

Yufang Xu ◽

Weiping Zhu ◽

Chunmei Yang ◽

Le Han ◽

...

Keyword(s):

Aqueous Solution ◽

Live Cells ◽

Turn On ◽

Fluorescence Turn On

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Highly selective fluorescence turn-on determination of Pb(II) in Water by in-situ enrichment of Pb(II) and MAPbBr3 perovskite growth in sulfydryl functionalized mesoporous alumina film

Sensors and Actuators B Chemical ◽

10.1016/j.snb.2020.128975 ◽

2021 ◽

Vol 326 ◽

pp. 128975

Author(s):

Shuya Wang ◽

Yipeng Huang ◽

Linchun Zhang ◽

Feiming Li ◽

Fangyuan Lin ◽

...

Keyword(s):

Mesoporous Alumina ◽

Alumina Film ◽

Turn On ◽

Fluorescence Turn On

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A novel pyridinium modified tetraphenylethene: AIE-activity, mechanochromism, DNA detection and mitochondrial imaging

Journal of Materials Chemistry B ◽

10.1039/c7tb03012f ◽

2018 ◽

Vol 6 (8) ◽

pp. 1279-1285 ◽

Cited By ~ 21

Author(s):

Zhaoyang Wang ◽

Yuan Gu ◽

Junyuan Liu ◽

Xiao Cheng ◽

Jing Zhi Sun ◽

...

Keyword(s):

Dna Binding ◽

Living Cell ◽

Cell Imaging ◽

Dna Detection ◽

Multiple Functions ◽

Turn On ◽

Fluorescence Turn On ◽

Living Cell Imaging

A cationic AIE-gen demonstrates multiple functions including mechanoluminochromic and solvatochromic effects, fluorescence turn-on responses to DNA-binding and mitochondria-specific living cell imaging.

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