scholarly journals Single-molecule spectroelectrochemical cross-correlation during redox cycling in recessed dual ring electrode zero-mode waveguides

2017 ◽  
Vol 8 (8) ◽  
pp. 5345-5355 ◽  
Author(s):  
Donghoon Han ◽  
Garrison M. Crouch ◽  
Kaiyu Fu ◽  
Lawrence P. Zaino III ◽  
Paul W Bohn
Keyword(s):  
Electron Transfer ◽  
Single Molecule ◽  
Cross Correlation ◽  
Zero Mode ◽  
Redox Cycling ◽  
Ring Electrode ◽  
Dual Ring

The ability of zero-mode waveguides (ZMW) to guide light into subwavelength-diameter nanoapertures has been exploited for studying electron transfer dynamics in zeptoliter-volume nanopores under single-molecule occupancy conditions.

Redox Cycling in Nanopore-Confined Recessed Dual-Ring Electrode Arrays

2016 ◽  
Vol 120 (37) ◽  
pp. 20634-20641 ◽  
Author(s):  
Donghoon Han ◽  
Lawrence P. Zaino ◽  
Kaiyu Fu ◽  
Paul W. Bohn
Keyword(s):  
Redox Cycling ◽  
Ring Electrode ◽  
Electrode Arrays ◽  
Dual Ring

Design and Fabrication of Linear-shaped Zero Mode Waveguides for Single Molecule Observation of Kinesin and Fluorescent ATP

2017 ◽  
Vol 137 (6) ◽  
pp. 159-164
Author(s):  
Kazuya Fujimoto ◽  
Yuki Morita ◽  
Ryota Iino ◽  
Michio Tomishige ◽  
Hirofumi Shintaku ◽  
...  
Keyword(s):  
Single Molecule ◽  
Zero Mode

Redox Cycling within Nanoparticle-Nucleated Protein Superstructures: Electron Transfer between Nanoparticulate Gold, Molecular Reductant, and Cytochrome c

2021 ◽  
Vol 125 (7) ◽  
pp. 1735-1745 ◽  
Author(s):  
Amanda S. Harper-Leatherman ◽  
Jean Marie Wallace ◽  
Jeffrey W. Long ◽  
Christopher P. Rhodes ◽  
Molly E. Graffam ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Cytochrome C ◽  
Redox Cycling

The Single-Molecule Conductance and Electrochemical Electron-Transfer Rate Are Related by a Power Law

ACS Nano ◽  
2013 ◽  
Vol 7 (6) ◽  
pp. 5391-5401 ◽  
Author(s):  
Emil Wierzbinski ◽  
Ravindra Venkatramani ◽  
Kathryn L. Davis ◽  
Silvia Bezer ◽  
Jing Kong ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Single Molecule ◽  
Power Law ◽  
Transfer Rate ◽  
Electron Transfer Rate

Probing the Influence of O2on Photoinduced Reversible Electron Transfer in Perylenediimide-Triphenylamine-Based Dendrimers by Single-Molecule Spectroscopy

2004 ◽  
Vol 43 (45) ◽  
pp. 6116-6120 ◽  
Author(s):  
Mircea Cotlet ◽  
Sadahiro Masuo ◽  
Marc Lor ◽  
Eduard Fron ◽  
Mark Van der Auweraer ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Single Molecule ◽  
Single Molecule Spectroscopy ◽  
Reversible Electron Transfer

scholarly journals Ripping RNA by Force using Gaussian Network Models

2016 ◽  
Author(s):  
Changbong Hyeon ◽  
D. Thirumalai
Keyword(s):  
Optical Tweezers ◽  
Single Molecule ◽  
Cross Correlation ◽  
Network Models ◽  
Force Extension ◽  
Correlation Matrices ◽  
Rna Molecules ◽  
Allosteric Communication ◽  
Ion Effects ◽  
Gaussian Network

AbstractUsing force as a probe to map the folding landscapes of RNA molecules has become a reality thanks to major advances in single molecule pulling experiments. Although the unfolding pathways under tension are complicated to predict studies in the context of proteins have shown that topology plays is the major determinant of the unfolding landscapes. By building on this finding we study the responses of RNA molecules to force by adapting Gaussian network model (GNM) that represents RNAs using a bead-spring network with isotropic interactions. Cross-correlation matrices of residue fluctuations, which are analytically calculated using GNM even upon application of mechanical force, show distinct allosteric communication as RNAs rupture. The model is used to calculate the force-extension curves at full thermodynamic equilibrium, and the corresponding unfolding pathways of four RNA molecules subject to a quasi-statically increased force. Our study finds that the analysis using GNM captures qualitatively the unfolding pathway of T. ribozyme elucidated by the optical tweezers measurement. However, the simple model is not sufficient to capture subtle features, such as bifurcation in the unfolding pathways or the ion effects, in the forced-unfolding of RNAs.

scholarly journals Single occupancy spectroelectrochemistry of freely diffusing flavin mononucleotide in zero-dimensional nanophotonic structures

2015 ◽  
Vol 184 ◽  
pp. 101-115 ◽  
Author(s):  
Lawrence P. Zaino ◽  
Dane A. Grismer ◽  
Donghoon Han ◽  
Garrison M. Crouch ◽  
Paul W. Bohn
Keyword(s):  
Electron Transfer ◽  
Single Molecule ◽  
Light Emission ◽  
Fluorescence Emission ◽  
Flavin Mononucleotide ◽  
Wide Field ◽  
Potential Sweep ◽  
Potential Control ◽  
Fluorescence Measurements ◽  
Electron Transfer Properties

Zero-mode waveguides (ZMW) have the potential to be powerful confinement tools for studying electron transfer dynamics at single molecule occupancy conditions. Flavin mononucleotide contains an isoalloxazine chromophore, which is fluorescent in the oxidized state (FMN) while the reduced state (FMNH2) exhibits dramatically lower light emission, i.e. a dark-state. This allows fluorescence emission to report the redox state of single FMN molecules, an observation that has been used previously to study single electron transfer events in surface-immobilized flavins and flavoenzymes, e.g. sarcosine oxidase, by direct wide-field imaging of ZMW arrays. Single molecule electron transfer dynamics have now been extended to the study of freely diffusing molecules using fluorescence measurements of Au ZMWs under single occupancy conditions. The Au in the ZMW serves both as an optical cladding layer and as the working electrode for potential control, thereby accessing single molecule electron transfer dynamics at μM concentrations. Consistent with expectations, the probability of observing single reduced molecules increases as the potential is scanned negative, Eappl < Eeq, and the probability of observing emitting oxidized molecules increases at Eappl > Eeq. Different single molecules exhibit different electron transfer properties as reflected in the position of Eeq and the distribution of Eeq among a population of FMN molecules. Two types of actively-controlled electroluminescence experiments were used: chronofluorometry experiments, in which the potential is alternately stepped between oxidizing and reducing potentials, and cyclic potential sweep fluorescence experiments, analogous to cyclic voltammetry, these latter experiments exhibiting a dramatic scan rate dependence with the slowest scan rates showing distinct intermediate states that are stable over a range of potentials. These states are assigned to flavosemiquinone species that are stabilized in the special environment of the ZMW nanopore.

Intramolecular Distances and Dynamics from the Combined Photon Statistics of Single-Molecule FRET and Photoinduced Electron Transfer

2013 ◽  
Vol 117 (42) ◽  
pp. 13015-13028 ◽  
Author(s):  
Dominik Haenni ◽  
Franziska Zosel ◽  
Luc Reymond ◽  
Daniel Nettels ◽  
Benjamin Schuler
Keyword(s):  
Electron Transfer ◽  
Single Molecule ◽  
Photoinduced Electron Transfer ◽  
Photon Statistics ◽  
Single Molecule Fret

scholarly journals Plasmonic zero mode waveguide for highly confined and enhanced fluorescence emission

Nanoscale ◽  
2018 ◽  
Vol 10 (36) ◽  
pp. 17362-17369 ◽  
Author(s):  
Paolo Ponzellini ◽  
Xavier Zambrana-Puyalto ◽  
Nicolò Maccaferri ◽  
Luca Lanzanò ◽  
Francesco De Angelis ◽  
...  
Keyword(s):  
Single Molecule ◽  
Experimental Verification ◽  
Zero Mode ◽  
Fluorescence Emission ◽  
Enhanced Fluorescence

Experimental verification of a plasmonic zero mode waveguide able to enhance fluorescence from a single molecule in a confined volume.

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