Intramolecular Distances and Dynamics from the Combined Photon Statistics of Single-Molecule FRET and Photoinduced Electron Transfer

2013 ◽  
Vol 117 (42) ◽  
pp. 13015-13028 ◽  
Author(s):  
Dominik Haenni ◽  
Franziska Zosel ◽  
Luc Reymond ◽  
Daniel Nettels ◽  
Benjamin Schuler
Keyword(s):  
Electron Transfer ◽  
Single Molecule ◽  
Photoinduced Electron Transfer ◽  
Photon Statistics ◽  
Single Molecule Fret

scholarly journals Two-colour single-molecule photoinduced electron transfer fluorescence imaging microscopy of chaperone dynamics

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jonathan Schubert ◽  
Andrea Schulze ◽  
Chrisostomos Prodromou ◽  
Hannes Neuweiler
Keyword(s):  
Electron Transfer ◽  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Conformational Changes ◽  
Photoinduced Electron Transfer ◽  
Structural Changes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescence Imaging Microscopy ◽  
Pet Probes

AbstractMany proteins are molecular machines, whose function is dependent on multiple conformational changes that are initiated and tightly controlled through biochemical stimuli. Their mechanistic understanding calls for spectroscopy that can probe simultaneously such structural coordinates. Here we present two-colour fluorescence microscopy in combination with photoinduced electron transfer (PET) probes as a method that simultaneously detects two structural coordinates in single protein molecules, one colour per coordinate. This contrasts with the commonly applied resonance energy transfer (FRET) technique that requires two colours per coordinate. We demonstrate the technique by directly and simultaneously observing three critical structural changes within the Hsp90 molecular chaperone machinery. Our results reveal synchronicity of conformational motions at remote sites during ATPase-driven closure of the Hsp90 molecular clamp, providing evidence for a cooperativity mechanism in the chaperone’s catalytic cycle. Single-molecule PET fluorescence microscopy opens up avenues in the multi-dimensional exploration of protein dynamics and allosteric mechanisms.

Photoinduced electron transfer (PET)-probes for the study of enzyme activity at the ensemble and single molecule level

2007 ◽  
Author(s):  
Sigrun Henkenjohann ◽  
Sebastian van de Linde ◽  
Sören Doose ◽  
Philip Tinnefeld ◽  
Markus Sauer
Keyword(s):  
Electron Transfer ◽  
Enzyme Activity ◽  
Single Molecule ◽  
Photoinduced Electron Transfer ◽  
Single Molecule Level ◽  
Pet Probes

Dual responsive chemosensors for anions: the combination of fluorescent PET (Photoinduced Electron Transfer) and colorimetric chemosensors in a single molecule

2003 ◽  
Vol 44 (35) ◽  
pp. 6575-6578 ◽  
Author(s):  
Thorfinnur Gunnlaugsson ◽  
Paul E. Kruger ◽  
T.Clive Lee ◽  
Raman Parkesh ◽  
Frederick M. Pfeffer ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Single Molecule ◽  
Photoinduced Electron Transfer ◽  
Dual Responsive

scholarly journals Photoinduced Electron Transfer of Oxazine 1/TiO2Nanoparticles at Single Molecule Level by Using Confocal Fluorescence Microscopy

Langmuir ◽  
2010 ◽  
Vol 26 (11) ◽  
pp. 9050-9060 ◽  
Author(s):  
Yi-Ju Chen ◽  
Hsin-Yu Tzeng ◽  
Hsiu-Fang Fan ◽  
Ming-Shiang Chen ◽  
Jer-Shing Huang ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Photoinduced Electron Transfer ◽  
Confocal Fluorescence Microscopy ◽  
Single Molecule Level ◽  
Confocal Fluorescence ◽  
Oxazine 1

Computationally Assisted Design of High Signal-to-Noise Photoinduced Electron Transfer-Based Voltage-Sensitive Dyes

2020 ◽  
Author(s):  
Rishikesh Kulkarni ◽  
Anneliese Gest ◽  
Chun Kei Lam ◽  
Benjamin Raliski ◽  
Feroz James ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Dft Calculations ◽  
Photoinduced Electron Transfer ◽  
Density Functional ◽  
Embryonic Stem ◽  
High Sensitivity ◽  
Md Simulations ◽  
High Signal ◽  
Signal To Noise ◽  
Green Fluorescent

<p>High signal-to-noise optical voltage indicators will enable simultaneous interrogation of membrane potential in large ensembles of neurons. However, design principles for voltage sensors with high sensitivity and brightness remain elusive, limiting the applicability of voltage imaging. In this paper, we use molecular dynamics (MD) simulations and density functional theory (DFT) calculations to guide the design of a bright and sensitive green-fluorescent voltage-sensitive fluorophore, or VoltageFluor (VF dye), that uses photoinduced electron transfer (PeT) as a voltage-sensing mechanism. MD simulations predict an 11% increase in sensitivity due to membrane orientation, while DFT calculations predict an increase in fluorescence quantum yield, but a decrease in sensitivity due to a decrease in rate of PeT. We confirm these predictions by synthesizing a new VF dye and demonstrating that it displays the expected improvements by doubling the brightness and retaining similar sensitivity to prior VF dyes. Combining theoretical predictions and experimental validation has resulted in the synthesis of the highest signal-to-noise green VF dye to date. We use this new voltage indicator to monitor the electrophysiological maturation of human embryonic stem cell-derived medium spiny neurons. </p>

Sign in / Sign up

Export Citation Format

Share Document